Estimating the post-mortem interval (II): The use of differential temporal gene expression to determine the age of blowfly pupae

Ames, Carole; Turner, Bryan; Daniel, Barbara

doi:10.1016/j.ics.2005.09.091

Cited by 18 publications

(15 citation statements)

References 3 publications

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“…Pupal age is usually estimated from color development of the puparium and external morphological examination of the pupa within (12–14). Gene expression analysis is also emerging as a possible molecular method of pupal age determination (10,11,15–17). In comparison with larvae whose age can be calculated to within a few hours based on length and width measurements, pupal age is at best estimated to the nearest day, with little published evidence to support this accuracy (8,18–21).…”

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confidence: 99%

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Internal Morphological Analysis for Age Estimation of Blow Fly Pupae (Diptera: Calliphoridae) in Postmortem Interval Estimation*

Davies

Harvey

2012

Journal of Forensic Sciences

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Larvae and pupae of blow fly species are frequently used in postmortem interval estimation, their age indicating minimum time since death. Most studies have considered age estimation of larvae, neglecting study of pupae. Relative development of external pupal features is useful, but there are also internal changes during metamorphosis that may be indicators of age, utilizing histological techniques. This study aimed to optimize preservation and histological analysis of blow fly pupae, specifically Calliphora vicina Robineau-Desvoidy and Lucilia sericata (Meigen), and to examine internal features with potential for age estimation. Effect of hot-water-killing and different preservatives were examined. It was determined that blow fly pupae should be pierced through the three tagma, hot-water-killed, and preserved in 80% ethanol as the optimal preservation for subsequent analyses. Hematoxylin and eosin stained pupal sections revealed differences in brain and thoracic muscle development throughout the pupal stage with potential for age estimation.

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mentioning

confidence: 99%

“…Pupal age is usually estimated from color development of the puparium and external morphological examination of the pupa within (12)(13)(14). Gene expression analysis is also emerging as a possible molecular method of pupal age determination (10,11,(15)(16)(17).…”

mentioning

confidence: 99%

Internal Morphological Analysis for Age Estimation of Blow Fly Pupae (Diptera: Calliphoridae) in Postmortem Interval Estimation*

Davies

Harvey

2012

Journal of Forensic Sciences

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“…However, later works extended to ageing the pupa [33], which is very useful as the size does not change during metamorphosis, and physical ageing is not possible. The accuracy of PMI estimates increases if ageing of the immature stages becomes more precise.…”

Section: Resultsmentioning

confidence: 99%

“…Real time PCR and differentially expressed genes have also been used in the determination of pupal age in Calliphora vicina [33]. This research indicated that expression of Arylphorin and Gene G genes is possible to determine the age of the immature stages.…”

Section: Determination Of Insect Developmental Ratementioning

confidence: 99%

Role of Polymerase Chain Reaction in Forensic Entomology

Chua¹,

Chong²

2012

Polymerase Chain Reaction

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“…Evidence that extracted from the study of arthropods can be employed in a forensic investigation in a variety of ways (Ames et al, 2006). The most important additive of forensic entomology is the estimation of the postmortem interval (PMI), event reconstructions and solving cases (Greenberg, 1991;Catts & Goff, 1992;Anderson & VanLaerhoven, 1996;Amendt et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Adli bilimlerde entomolojinin uygulanması

Kökdener¹

2016

Türk. entomol. bült.

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* SummaryForensic entomology is the use of the insects in legal purposes is becoming increasingly more valuable in criminal investigations. Insects are attracted to the body immediately after death and lay eggs in it. Forensic entomologist use knowledge of insect to solve crimes and insect evidences may shed light on different aspects of the crimes. This science emerged as a major discipline in the developed countries and its role in criminal investigations became more widespread. Nowadays, forensic entomologists are called upon more frequently to refer their knowledge and expertise and to collaborate in criminal investigations and to become important part of forensic investigation teams. Unfortunately, it has not received much attention in Turkey as an important investigative tool. In spite of this major potential, however, the field of forensic entomology uncertain in our country, largely. There are lack of knowledge of the benefits, application and hesitation on practically in our country. This study aims to describe the principles and concept of forensic entomology, to determine the usefulness and applicability of insect evidence, to develop awareness of insect and to attract attention of forensic medicine professionals to the issue. Key words: Forensic entomology, decomposition, postmortem interval, arthropods ÖzetBöceklerin adli amaç için kullanımı olan adli entomoloji olay yeri incelemelerinde oldukça artan bir öneme sahiptir. Böcekler ölümden sonra cesede hemen ulaşırlar ve yumurtlarlar. Adli entomolog suçları çözmek için böcek bilgisini kullanır ve böcek delilleri suçun farklı yönlerine ışık tutabilirler. Bu bilim dalı gelişmiş ülkelerde önemli disiplin olarak ortaya çıkmış ve yasal soruşturmalardaki rolü yaygınlaşmıştır. Günümüzde adli entomologlar uzmanlıklarından yararlanmak, işbirliklerine başvurmak üzere adli araştırmalara çağrılmakta ve adli araştırma ekibinin önemli bir kısmını oluşturmaktadırlar. Ne yazık ki Türkiye' de adli entomolojinin suç araştırmalarda önemli bir araç olarak kullanımı yeterince dikkat çekmemiştir. Ülkemizde adli entomolojinin uygulaması hakkında bilgi eksikliği ve tereddütlerin olmasından kaynaklanan belirsizlikler vardır. Bu çalışma adli entomoloji ilkelerini ve içeriğini tanımlamak, böcek delillerinin uygulama alanlarını, faydalarını belirlemek, böceklerin farkındalığını arttırmak ve adli tıp profesyonellerin ilgisini bu alana çekmeyi amaçlamıştır.Anahtar sözcükler: Adli entomoloji, çürüme, ölüm sonrası geçen zaman, böcekler

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Estimating the post-mortem interval (II): The use of differential temporal gene expression to determine the age of blowfly pupae

Cited by 18 publications

References 3 publications

Internal Morphological Analysis for Age Estimation of Blow Fly Pupae (Diptera: Calliphoridae) in Postmortem Interval Estimation*

Internal Morphological Analysis for Age Estimation of Blow Fly Pupae (Diptera: Calliphoridae) in Postmortem Interval Estimation*

Role of Polymerase Chain Reaction in Forensic Entomology

Adli bilimlerde entomolojinin uygulanması

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