Estimating Genotypic Diversity of Streptococcus mutans Isolated From Caries-Active and Caries-Free Individuals Among Indian Population

Abstract

To identify the genetic characterization of S mutans strains isolated from the caries-free and caries-active population using arbitrarily primed -polymerase chain reaction (AP-PCR). Materials and methodsThirty-five subjects with a decayed missing and filled teeth (DMFT) score of 0 were allocated to the cariesfree group and 35 subjects with a DMFT score greater than four were allocated to the caries-active group and salivary samples were collected. The samples were processed and the colony forming units (CFU) of S mutans were determined for all the samples. About three colonies resembling S mutans from each subject were subjected to deoxyribonucleic acid (DNA) isolation, a purification process was performed, and DNA was analyzed by AP-PCR. ResultsAmong the 210 isolates from 35 caries-active and 35 caries-free subjects that were analyzed by AP-PCR, 41.9% had a single genotype, 25.8% had two genotypes, 19.4% had three genotypes, 9.7% had four genotypes and 3.2% had more than four genotypes among the caries-free group. Among the caries-active group, 54.3% had a single genotype, 25.7% had two genotypes, 14.3% had three genotypes and 5.7% had four genotypes. ConclusionThe study concluded that different genotypic pattern was present in caries-free and caries-active subjects which indicate the occurrence of genetic polymorphism among the S mutans strains. There was no particular genotype of S mutans that was uniquely present in subjects in caries-active and caries-free individuals.

Beneficial modulation of human health in the oral cavity and beyond using bacteriocin-like inhibitory substance-producing streptococcal probiotics

“…The range of specific probiotic applications of BLIS M18 have largely been prompted by idiosyncrasies of its BLIS spectrum (Supplementary Table B). Successful outcomes have been reported not only for its impact on Cariogram index (Di Pierro et al, 2015b;Tandelilin et al, 2018;Poorni et al, 2022), reduction of gingivitis/periodontitis (Scariya et al, 2015) and halitosis (Benic et al, 2019;Yoo et al, 2020) and modulation of black staining plaque (Gobbi et al, 2020), but also for its unanticipated postbiotic activity against colon cancer cells ( Karaçam and Tunçer, 2022) and the important Gram-negative pathogens P. aeruginosa and Klebsiella pneumoniae (Tunçer and Karaçam, 2020).…”