Establishment of Pregnancy in the Pig: III. Endometrial Secretory Response to Estradiol Valerate Administered on Day 11 of the Estrous Cycle1,2,3

Geisert, R. D.; Thatcher, W.W.; Roberts, R. Michael; Bazer, Fuller W.

doi:10.1095/biolreprod27.4.957

Cited by 143 publications

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“…Those in the pregnant group were bred when detected in estrus and 12 and 24 h thereafter. Pigs were hysterectomized either on day 5, 9, 12, or 15 of the estrous cycle or day 9,10,12,13,14,15,20,25,30,35,40,60, or 85 of pregnancy (n ϭ 3 pigs/day/status). Study two.…”

Section: Figurementioning

confidence: 99%

“…of either 5 ml of corn oil vehicle or 5 mg of 17␤-estradiol benzoate (5 mg in 5 ml of corn oil; Sigma-Aldrich) on days 11-14 postestrus (n ϭ 5 pigs/ treatment). This dose of 17␤-estradiol is used to induce pseudopregnancy in pigs (25). All pigs were hysterectomized on day 15 postestrus.…”

Section: Figurementioning

confidence: 99%

“…In addition, conceptus estrogens modulate uterine gene expression responsible for uterine remodeling for implantation and placentation from days 13 to 25 of gestation (25). The importance of estrogen is underscored by the fact that premature exposure of the pregnant uterus to estrogen on days 9 and 10 results in degeneration of all pig conceptuses by day 15 (26).…”

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confidence: 99%

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Uterine MHC Class I Molecules and β2-Microglobulin Are Regulated by Progesterone and Conceptus Interferons during Pig Pregnancy

Joyce

Burghardt

et al. 2008

The Journal of Immunology

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MHC class I molecules and β2-microglobulin (β2m) are membrane glycoproteins that present peptide Ags to TCRs, and bind to inhibitory and activating receptors on NK cells and other leukocytes. They are involved in the discrimination of self from non-self. Modification of these molecules in the placenta benefits pregnancy, but little is known about their genes in the uterus. We examined the classical class I swine leukocyte Ags (SLA) genes SLA-1, SLA-2, and SLA-3, the nonclassical SLA-6, SLA-7, and SLA-8 genes, and the β2m gene in pig uterus during pregnancy. Uterine SLA and β2m increased in luminal epithelium between days 5 and 9, then decreased between days 15 and 20. By day 15 of pregnancy, SLA and β2m increased in stroma and remained detectable through day 40. To determine effects of estrogens, which are secreted by conceptuses to prevent corpus luteum regression, nonpregnant pigs were treated with estradiol benzoate, which did not affect the SLA or β2m genes. In contrast, progesterone, which is secreted by corpora lutea, increased SLA and β2m in luminal epithelium, whereas a progesterone receptor antagonist (ZK137,316) ablated this up-regulation. To determine effects of conceptus secretory proteins (CSP) containing IFN-δ and IFN-γ, nonpregnant pigs were implanted with mini-osmotic pumps that delivered CSP to uterine horns. CSP increased SLA and β2m in stroma. Cell-type specific regulation of SLA and β2m genes by progesterone and IFNs suggests that placental secretions control expression of immune regulatory molecules on uterine cells to provide an immunologically favorable environment for survival of the fetal-placental semiallograft.

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Section: Figurementioning

confidence: 99%

Section: Figurementioning

confidence: 99%

mentioning

confidence: 99%

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Uterine MHC Class I Molecules and β2-Microglobulin Are Regulated by Progesterone and Conceptus Interferons during Pig Pregnancy

Joyce

Burghardt

et al. 2008

The Journal of Immunology

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“…Pregnancy recognition is the result of conceptus secretion of estrogens on days 11 and 12 of pregnancy to redirect PGF secretion from the uterine vasculature to the uterine lumen. In addition to pregnancy recognition, conceptus estrogens modulate uterine gene expression that is essential for conceptus development and implantation (Geisert et al 1982). Pig Tr also secretes IFNs, both the type I IFNd and the type II IFNg during the peri-implantation period (La Bonnardière et al 1991, Lefèvre et al 1998, Joyce et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

et al. 2010

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Pigs experience significant conceptus loss near mid-gestation, correlating with increasing glandular epithelial (GE) development and secretory activity. Secreted phosphoprotein 1 (SPP1, osteopontin) increases in GE between days 30 and 40 of pregnancy and is expressed in the GE of day 90 pseudopregnant pigs, suggesting that progesterone (P 4 ) from corpora lutea is responsible for induction of SPP1 in GE. In this study, pigs were ovariectomized and treated daily with P 4 to assess effects of 40 days of P 4 exposure on SPP1, P 4 receptor (PGR), uteroferrin (ACP5), and fibroblast growth factor 7 (FGF7) expression in porcine endometria. PGR mRNA decreased in pigs injected with P 4 compared with pigs injected with corn oil (CO), and PGRs were downregulated in the luminal epithelium (LE) and GE. ACP5 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and ACP5 was induced in the GE of P 4 -treated pigs. FGF7 mRNA increased in pigs injected with P 4 compared with pigs injected with CO, and FGF7 was induced in the LE and GE of P 4 -treated pigs. SPP1 mRNA was not different between pigs injected with P 4 compared with pigs injected with CO, and SPP1 was not present in the GE of P 4 -treated pigs. Therefore, long-term P 4 , in the absence of ovarian and/or conceptus factors, does not induce SPP1 expression in GE. We hypothesize that a servomechanism involving sequential effects of multiple hormones and cytokines, similar to those for sheep and humans, is required for GE differentiation and function, including the synthesis and secretion of SPP1.

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“…This period is also known as the maternal recognition of pregnancy in pigs. It has been hypothesized that an increase in estrogen concentration stimulates the secretion of endometrial proteins, promoting trophoblast growth, which are critical for implantation [8].…”

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Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

Jalali

Bogacki

Dietrich

et al. 2015

Journal of Proteomics

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Establishment of Pregnancy in the Pig: III. Endometrial Secretory Response to Estradiol Valerate Administered on Day 11 of the Estrous Cycle1,2,3

Cited by 143 publications

References 0 publications

Uterine MHC Class I Molecules and β2-Microglobulin Are Regulated by Progesterone and Conceptus Interferons during Pig Pregnancy

Uterine MHC Class I Molecules and β2-Microglobulin Are Regulated by Progesterone and Conceptus Interferons during Pig Pregnancy

Effects of long-term progesterone exposure on porcine uterine gene expression: progesterone alone does not induce secreted phosphoprotein 1 (osteopontin) in glandular epithelium

Proteomic analysis of porcine endometrial tissue during peri-implantation period reveals altered protein abundance

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