Establishment of peritoneal liquid electrophoretogram from healthy horses and horses submitted to experimentally induced intestinal obstruction

Abstract

The initial inflammatory stages of the colic syndrome include changes known as acute phase response. The aim of this study was to contribute with the establishment of reference values concerning the electrophoretogram of peritoneal liquid from healthy horses and horses submitted to experimentally induced intestinal obstruction. Twenty-one horses were allotted in four groups: duodenal obstruction (DG), ileum obstruction (IG), left-dorsal colon obstruction (MG), and control group (CG). Peritoneal liquid was sampled before obtruction (T0), with 3 hours of obstruction (T3) and 6, 30, 102 and 174 hours after desobstructing (T6, T30, T102 and T174, respectively). Total protein levels were determined by the biuret method and protein fractions were obtained by SDS-PAGE electrophoresis. The acute phase proteins (APP) identified were Immunoglobulin-A, ceruloplasmin, transferrin, albumin, α1-antitrypsin, heavy and light chains of immunoglobulin-G, haptoglobin, α1-acid glycoprotein and a still unnamed protein, which was called P24. There was no difference (P>0.3) in protein levels among groups, although a significant difference (P>0.05) was observed between distinct experimental moments in each group evidencing a higher response of the APP in the obstructed groups. The APP fractioning of the peritoneal liquid was standardized to establish a standard curve for healthy equines and those submitted to induced intestinal obstruction. Moreover, it was verified that the SDS-PAGE electrophoresis was sensitive and effective to help diagnose abdominal inflammatory processes.

Acute phase proteins levels in horses, after a single carbohydrate overload, associated with cecal alkalinization

“…Similar elevation patterns of Trf were found by Barros et al ( 33 ) in donkeys submitted to laparoscopic ovariectomy and by Nogueira et al ( 26 ) in the peritoneal fluid of horses with intestinal obstruction. When evaluating the effect of treatment not associated with the inflammatory condition (BG × CG), it is noted an increase in Trf concentration in the animals treated with buffer solution, up to 16 h after its administration (T12h and T24h).…”

“…Based on ceruloplasmin (Cp) molecular weight, this protein should have been identified in the 130 kDa protein fraction on the SDS-PAGE gel. This section was cut from polyacrylamide gel, submitted to LC-MS ( 26 – 28 ), but it did not bring a reliable identification. Assuming that the location of Cp in the protein fraction matches the literature ( 26 – 28 ), we chose to maintain the results of the alleged ceruloplasmin (aCp) in this study, which was obtained by densitometry analysis from the SDS-PAGE.…”

Validação de kits bioquímicos comerciais humanos para mensuração do proteinograma e eletrólitos séricos em equinos

“…Resultados semelhantes foram observados por Saquetti et al (2008), em equinos submetidos à obstrução experimental do cólon maior. Entretanto, a TRF se mostrou aumentada no líquido amniótico de éguas com placentite (LOUX e BALL, 2018), no soro de cavalos de salto submetidos a treinamento de alta intensidade (ASSENZA et al, 2016), líquido peritoneal de asininos submetidos a ovariectomia (BARROS et al, 2018) e em equinos com obstrução intestinal (NOGUEIRA et al, 2014).…”