Establishment of an allergic rhinitis model in mice for the evaluation of nasal symptoms

Tsunematsu, Miwako; Yamaji, Taketo; Kozutsumi, Daisuke; Murakami, Rika; Kimura, Shigeki; Kino, Kohsuke

doi:10.1016/j.lfs.2006.12.038

Cited by 34 publications

(32 citation statements)

References 30 publications

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“…Previous studies have reported many pollen allergy models. 13,[17][18][19][20][21] Some of these studies could induce allergy without using any adjuvants. …”

Section: Discussionmentioning

confidence: 99%

“…[17][18][19] However, the sensitizing mechanism occurring in the model would be different from that in patients with pollen allergy who had been sensitized by inhaling the antigen. That model would therefore be inappropriate for analyzing the mechanism involved in the onset of pollen allergy and investigating the effect and mechanism of anti-allergic foods or medicines for pollen allergy.…”

Section: Antigen Stimulation( G/ml)mentioning

confidence: 99%

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Establishment and characterization of a novel murine model for pollen allergy

Murakami

Nakayama

Hattori

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Although there have been many studies revealing the mechanism and establishing the therapeutical method for allergic rhinitis, no suitable animal models for allergic rhinitis, especially for pollen allergy, are currently available. We therefore aimed in this study to develop a murine model producing IgE in response to an inhaled antigen without using any adjuvants. Ovalbumin (OVA)-specific T cell receptor transgenic mice (DO11.10) inhaled an OVA solution for one h, twice a week, for six weeks. The resulting increase of OVA-specific IgE in the serum was observed depending on the times of inhalation. Spleen cells from mice that had inhaled the antigen produced more IL-4 and less IFN-γ than those from the control mice in vitro. These results indicate that inhaled antigen enhanced the Th2-type responses and induced IgE production in a T cell-mediated manner. Our findings would contribute to studies on prevention and treatment of pollen allergy.

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“…Previous studies have reported many pollen allergy models. 13,[17][18][19][20][21] Some of these studies could induce allergy without using any adjuvants. …”

Section: Discussionmentioning

confidence: 99%

Section: Antigen Stimulation( G/ml)mentioning

confidence: 99%

Establishment and characterization of a novel murine model for pollen allergy

Murakami

Nakayama

Hattori

et al. 2015

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

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“…Sensitization was confirmed by induction of serum total IgE which was not detected in the serum of an unprimed mouse. The mouse model was reported to be suitable as a mouse model developing Japanese cedar pollinosis with nasal symptoms (24), and its symptoms to be reduced by inoculation of peptide 7crp (16,17). We examined the therapeutic effect of transcutaneous administration of 7crpR to a rhinitis mouse model.…”

Section: Therapeutic Treatment Of Pollinosis Mouse Modelsmentioning

confidence: 99%

Transcutaneous Peptide Immunotherapy of Japanese Cedar Pollinosis Using Solid-in-Oil Nanodispersion Technology

et al. 2015

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Abstract. Peptide immunotherapy is an attractive approach to relieve allergic symptoms such as rhinitis and asthma. Treatment of Japanse cedar pollinosis (Cryptomeria japonica; Cj), from which over one quarter of Japanese population suffer, is becoming a great concern. Recently, oral feeding of a peptide (7crp) consisting of seven immunodominant human T cell epitopes derived from two enzymes present in Cj pollen was demonstrated to have a benefit in treating Cj pollinosis. In this work, we aimed to apply a novel transcutaneous administration system as a simple and easy peptide delivery for an immunotherapy using a T cell epitope peptide. A modified 7crp peptide (7crpR) which contained triarginine linkers between each epitopes was designed to increase water solubility and was encapsulated in a unique solid-in-oil (S/O) nanodispersion. The S/O nanodispersion consists of a nano-sized peptide-surfactant complex dispersed in an oil vehicle. The S/O nanopartilces having an average diameter of 230 nm facilitated the permeation of the peptide 7crpR into the skin and suppressed serum total IgE and antigen-specific IgE levels in a Cj pollinosis mouse model. Transcutaneous administration of the T cell epitope peptide using the S/O nanodispersion system has potential for future simple and easy immunotherapy of Cj pollinosis.

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“…In the allergic guinea pig, enumerating the frequency of nasal rubbing and sneezes is a subjective but useful measure, especially for testing the early phase mechanisms and therapies involving histamine-and leukotriene-dependent pathways (Al Suleimani et al 2006;Szelenyi et al 2000). For example observers will count between 3-6 sneezes and 6-10 rubbings per minute after acute exposure to allergen (Al Suleimani et al 2008;Tsunematsu et al 2007). However, the histopathology associated with nasal obstruction in both early and late phase responses in AR has not been extensively studied.…”

Section: Animal Models Of Allergic Rhinitismentioning

confidence: 99%