Establishment of a leukaemic cell line from a patient with acquisition of chromosomal abnormalities during disease progression in myelodysplastic syndrome

Abstract

Summary. A cell line designated SKM‐1 was newly established from leukaemic cells of a 76‐year‐old Japanese male patient with monoblastic leukaemia following myelodysplastic syndrome (MDS). The cells were obtained from peripheral blood of the patient when he lost multiple point mutations of ras genes with acquisition of chromosomal abnormalities during disease progression in MDS. The cells grew as a single floating cell, and have been continuously growing with the morphological characteristics of immature monoblasts by serial passages during the past 42 months with a doubling time of about 48 h. By cytochemical analysis. the cloned cells were positive for butyrate esterase, but negative for the Epstein‐Barr virus associated nuclear antigen. Phenotypic analysis revealed the expression of myelomonocyte specific antigens such as CD4, CD13, CD33 and HLA‐DR. Cells from the primary peripheral blood and those from SO passages of the SKM‐1 cell line both possessed no activated ras genes but showed karyotype abnormalities with 46.XY, del(9)(q13;q22), der(17) t(17:?)(p13:?). The SKM‐1 cells have two mutations in p53 gene and overexpress the pS3 products. This cell line may contribute to a better understanding of molecular mechanisms in the progression from MDS to myelogenous leukaemia.

Effect of magnetic Fe3O4 nanoparticles with 2-methoxyestradiol on the cell-cycle progression and apoptosis of myelodysplastic syndrome cells

“…19 The cells were cultured in RPMI-1640 containing 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin in an environment of saturated humidity, 5% CO 2 , and 37°C. The cells in the logarithmic growth phase were used in all experiments.…”

Downregulation of hTERT: An Important As2O3 Induced Mechanism of Apoptosis in Myelodysplastic Syndrome

“…The SKM-1 cell line (JCRB0118; Japanese Collection of Research Bioresources Cell Bank, Osaka, Japan) was established from leukemic cells of a 76-year-old Japanese male patient with monoblastic leukemia following myelodysplastic syndrome [21]. The cells were cultured in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal calf serum, 50 IU/ml penicillin, and 50 µg/ml streptomycin at 37°C with 5% CO 2 in air.…”

“…Thus, based on the evidence provided by previous studies, we speculated that As 2 O 3 -induced apoptosis involves regulation of components in signal transduction pathways, such as the hTERT and NF-κB pathways, in leukemia cells. In the present study, we examined the effect of As 2 O 3 on the survival and apoptosis of MUTZ-1 and SKM-1 cells, two MDS derived cell lines [20], [21]. In addition, we determined a possible molecular mechanism of As 2 O 3 -induced apoptosis by evaluating the expression levels of hTERT and NF-κB in MDS undergoing apoptosis.…”

Different Mechanisms of Drug Resistance in Myelodysplastic Syndromes and Acute Myeloid Leukemia

“…Both these cell lines were derived from AML patients, whose disease developed from MDS. In contrast to MOLM-13 with wild type of p53 [63,64], SKM-1 represents cells expressing a mutated p53 form [65]. Thus cells with mutated TP53 could express P-gp under long-term LEN treatment, which leads to typical P-gp mediated MDR.…”