Establishment and characterization of a human hybridoma secreting monoclonal antibody with high titers of sperm immobilizing and agglutinating activities against human seminal plasma

Isojima, Shinzo; Kameda, K.; Tsuji, Yoshiyuki; Shigeta, Minoru; Ikeda, Yoshikazu; Koyama, Koji

doi:10.1016/0165-0378(87)90051-9

Cited by 90 publications

(47 citation statements)

References 6 publications

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“…CD52 has been identified as a potential target for antisperm carbohydrate antibodies involved in immune infertility [75]. In a benchmark study, Isojima et al [86] established a human-mouse heterohybridoma using peripheral blood lymphocytes from an infertile woman who exhibited high sperm-inhibitory titers. …”

Section: Immune Infertilitymentioning

confidence: 99%

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Ab¹

2003

CMLS, Cell. Mol. Life Sci.

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Glycoconjugates in the mammalian reproductive tract are critical components of the molecular mechanisms that control sperm maturation, sperm transport and gamete interactions. In the oviduct of many species, sperm transport and maturation are regulated by protein-carbohydrate interactions that form a sperm reservoir. Subsequently, gamete interactions are mediated by the binding of lectin-like sperm proteins with carbohydrate moieties on the zona pellucida. The sperm glycocalyx is extensively modified during sperm transport and maturation. Multiple functions have been proposed for this dense carbohydrate layer overlying the sperm plasmalemma, and sperm-surface carbohydrates have been implicated in immune-mediated human infertility. The structure and function of glycoconjugates in the oviductal sperm reservoir, the zona pellucida, and on the sperm surface are reviewed.

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Section: Immune Infertilitymentioning

confidence: 99%

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Ab¹

2003

CMLS, Cell. Mol. Life Sci.

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“…The absorbance was determined at 495 nm using an RM ELISA reader (Dynatech AG, Branch Germany, Denkendorf, Germany). For sperm ELISA (Isojima et al, 1987), the microELISA plates were filled with 50 pi of a suspension of washed boar, rabbit or bull ejaculated spermatozoa (6 IO6 cells ml-1), air dried at room temperature and incubated overnight at 4°C. Protein binding sites of the wells were blocked by blocking buffer (PBS, 1 % BSA, 2% normal porcine serum) for 1 h at 22°C.…”

Section: Capacitationmentioning

confidence: 99%

Binding of a 15 kDa glycoprotein from spermatozoa of boars to surface of zona pellucida and cumulus oophorus cells

Veselský

Jonáková²,

Ml³

et al. 1992

Reproduction

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A highly purified 15 kDa glycoprotein isolated from ejaculated spermatozoa was used to raise antisera in female rabbits. An indirect immunofluorescence technique was used to detect the antigen in the seminal vesicle tissue and on the acrosomes of ejaculated, native and capacitated, boar spermatozoa. No immunoreactivity was detected on cells of the seminiferous tubules (spermatogonia, spermatocytes, and spermatids), on spermatozoa in the ductus epididymis and in cells of the epididymal and testicular tissues. These observations support the view that the 15 kDa protein is produced in the seminal vesicle secretory epithelium, and is attached to the sperm plasma membrane during the exposure of spermatozoa to seminal vesicle compounds. The observations that the antigen remained on the acrosome of ejaculated spermatozoa after capacitation and blocked sperm-oocyte binding in vitro suggest that the antigen plays a role in sperm-egg interactions. The strong immunoreactivity exhibited by cumulus cells after incubation of antisera with the porcine egg surrounded by cumulus cells shows the possible importance of the 15 kDa glycoprotein for contact of spermatozoa with cells of the cumulus oophorus surrounding the egg.

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“…Human IgG has a half‐life of 21 days. Recently, there has been a growing interest to humanize the murine monoclonal antibodies or to raise human monoclonal antibodies against various sperm contraceptive epitopes (Isojima et al, 1987; Clayton et al, 1998; Norton et al, 2001). These antibodies, and additional antibodies that are being engineered, await trials in animal models and in humans for demonstration of efficacy, duration, and reversibility of the antifertility effects.…”

Section: Sperm Antigensmentioning

confidence: 99%

Antisperm Immunity for Contraception

Naz¹

2006

Journal of Andrology

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Establishment and characterization of a human hybridoma secreting monoclonal antibody with high titers of sperm immobilizing and agglutinating activities against human seminal plasma

Cited by 90 publications

References 6 publications

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Glycoconjugates in sperm function and gamete interactions: how much sugar does it take to sweet-talk the egg?

Binding of a 15 kDa glycoprotein from spermatozoa of boars to surface of zona pellucida and cumulus oophorus cells

Antisperm Immunity for Contraception

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