Estabelecimento De Um Modelo De Tumor Experimental Pela Inoculação Do Tumor De Walker Em Estômago De Rato

Oliveira, Paulo Ferdinando de Melo; Henriques, Iuri Aderaldo; Filho, Filadelfo Rodrigues; Almeida, Paulo Roberto Carvalho de; Moraes, Manoel Odoríco de

doi:10.1590/s0102-86501998000400008

Cited by 7 publications

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References 9 publications

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“…Without previous acid acetic inoculation the lesion is inconsistent and uneven, with tumor growth on few rats. That agrees with Oliveira et al 7 fi ndings where the inoculation on gastric mucosa was only made possible with a previous lesion and Dornelas et al 10 fi ndings where tumor implantation happened only on the spot where the bladder was injured, demonstrating that unharmed mucosa does not allow tumor implantation. On both groups B and C the tumor implantation rate was 100%, without difference between concentrations, similar to other inoculation Walker 256 carcinoma models, like kidney, 9 bladder, 10 stomach, 7 mouth 11 and others.…”

Section: Discussionsupporting

confidence: 86%

“…(insert references) It is a well described neoplasm, easily maintained in laboratory conditions, has fast and uniform growing, rarely regresses, and its effi ciency has been already confi rmed in therapeutical tests. 5 There are numerous Walker 256 carcinoma implant site models, such as skin, 6 muscle, 5 subcutaneous tissue, 5 gastric walls, 7 lungs, 8 kidneys, 9 among others. However, to the best of our knowledge, there are no reports of gynecological Walker carcinomas.…”

Section: Introductionmentioning

confidence: 99%

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Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Brito

Carvalho

et al. 2007

Acta Cir. Bras.

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Purpose:To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. Methods: Fifteen female rats were used, and assigned to three groups each one with fi ve rats: group A -rats with 4x10 6 cells of Walker 256 carcinoma without acid acetic inoculation; group B -rats with 2x10 6 cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10 6 cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance -ANOVA. P values < 0.05 were taken to indicate statistical signifi cance. Results: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. Conclusion: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10 6 or 4x10 6 cells on its inoculation. Key words: Carcinoma 256, Walker. Cervix uteri. Vagina. Disease models, animal. Rats. RESUMOObjetivo: Estabelecer um modelo de inoculação de Tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 15 ratas fêmeas, virgens, adultas, pesando entre 200-250g, distribuídas em três grupos de estudo com cinco animais cada: grupo A (GA): ratas com tumor de Walker 256 em concentração de 4x10 6 sem ácido acético; grupo B (GB): ratas com tumor de Walker 256 em concentração de 2x10 6 células com ácido acético; grupo C (GC): ratas com tumor de Walker 256 em concentração de 4x10 6 células com ácido acético. No dia anterior à inoculação do tumor, foi realizada a inoculação de 0,3 ml de ácido acético a 10% na vagina das ratas de GB e GC; no dia seguinte, tanto estas como as ratas do grupo GA foram anestesiadas, feita a escarifi cação da parede vaginal com uma escova de endocérvice e inoculado 0,3ml de tumor na concentração de 4x10 6 células nos grupos GA e GC e 2x10 6 células no grupo GB. Após 12 dias, foi realizada a eutanásia e removido o tumor em bloco com vagina e cornos uterinos para análise, sendo pesado e averiguado seu volume e calculado as relaçõe...

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Brito

Carvalho

et al. 2007

Acta Cir. Bras.

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“…Desde então ele vem sendo mantido em diversos laboratórios no mundo inteiro como um recurso valioso no auxílio à pesquisa básica em oncologia. São numerosos os trabalhos sobre o tumor de Walker, seu comportamento biológico, suas alterações bioquímicas e efeitos metabólicos 2,3 , e sua utilização em modelos experimentais no pulmão 4 , estômago 5 , rim 6 , cavidade oral 7 e fígado 8 . Nosso objetivo é o estudo de um modelo experimental para Walker em bexiga.…”

Section: Introductionunclassified

Modelo experimental do carcinossarcoma 256 de Walker em bexiga de ratos

et al. 2006

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RESUMO ABSTRACTPurpose: An animal model to study bladder tumor with Walker 256. Methods: Bladders rats Wistar was catheterized via urethra and compression lesion of the wall bladder was realized with a little clamping after laparotomy. One total of the 0,3 ml suspension with 3 x 10 5 cels viable of the carcinossarcoma was instilled into each bladder. The animals were sacrificed after eight and 13 days. Results: The index of tumor incidence was 100% and the average of surviving was 14,5 days. Conclusion: The model estudied was efficient and will can to take subsidy the study experiemental in treatment of local invasise bladder cancer.

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“…That may be due to the fact that scarified tissues promote an increase on the take rate of the tumor 14 ; thus, the presence of strings used for wiring of uterine horns could have influenced the extension of tumor all the way to the uterine horns.…”

Section: Discussionmentioning

confidence: 99%

The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

et al. 2009

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Purpose: Verify the effect of oophorectomy on the evolution of the Walker 256 tumor inoculated into the vagina and cervix of female rats. Methods: Ten Wistar, female rats were used, distributed into two groups with 05 animals each: Tumor group (TG): Rats inoculated with Walker 256 tumor; Oophorectomy group (OG): oophorectomized rats inoculated with Walker 256 tumor. The day before the tumor vaginal inoculation, acetic acid was inoculated into the vaginas of both groups of rats; the following day, the vaginal walls were scarified with an endocervix brush, and then Walker 256 tumor was inoculated. After 12 days, the tumor was removed together with the vagina and uterine horns for macro and microscopic analyses. The data were submitted to statistical analyses. Results: There was no statistical difference between the two groups; however it was observed that the behavior of tumor growth on the OG group presented greater invasion, compromising the uterine horns. Conclusion: The results of the study on the GO group presented a macroscopic behavior different from the TG group, however, both of them presented similar development in terms of tumor mass. Keywords: Ovariectomy. Cervix Uteri. Vagina. Carcinoma 256, Walker. Rats. RESUMOObjetivo: Verificar o efeito da ooforectomia à inoculação do tumor de Walker 256 em vagina e colo de útero de ratas. Métodos: Foram utilizadas 10 ratas Wistar, fêmeas, virgens, adultas, distribuídas em dois grupos de estudo com 05 animais cada: grupo tumor (GT): ratas inoculadas com tumor de Walker 256, e grupo Ooforectomia (GO): ratas ooforectomizadas e inoculadas com tumor de Walker 256. No dia anterior à inoculação vaginal do tumor, foram inoculados 0,3ml de ácido acético na vagina das ratas de ambos os grupos; no dia seguinte, foi realizada a escarificação da parede vaginal com uma escova de endocérvice e inoculado tumor de Walker 256. Após 12 dias, foi removido o tumor em bloco com vagina e cornos uterinos para análise macro e microscópica. Os dados foram submetidos à análise estatística. Resultados: Não houve diferença estatística entre os dois grupos; no entanto, notou-se que o comportamento de crescimento do tumor no grupo GO apresentou maior invasividade em extensão, comprometendo cornos uterinos. Conclusão: Observou-se que o grupo GO apresentou comportamento macroscópico diferente ao grupo GT, no entanto, em termos totais de massa tumoral ambos apresentam desenvolvimento similar. Descritores: Ovariectomia. Colo do Útero. Vagina. Carcinoma 256 Walker. Rats.

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Estabelecimento De Um Modelo De Tumor Experimental Pela Inoculação Do Tumor De Walker Em Estômago De Rato

Cited by 7 publications

References 9 publications

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Modelo experimental do carcinossarcoma 256 de Walker em bexiga de ratos

The oophorectomy effect on Walker 256 tumor inoculated into the vagina and uterine cervix of female rats

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