Espirito Santo Virus: a New Birnavirus That Replicates in Insect Cells

Vancini, Ricardo; Paredes, Angel; Ribeiro, Mariana; Blackburn, Kevin; Ferreira, Davis Fernandes; Kononchik, Joseph; Hernandez, Raquel; Brown, Dennis T.

doi:10.1128/jvi.06614-11

Cited by 21 publications

(36 citation statements)

References 48 publications

(59 reference statements)

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“…Alignment of various pVP2 CTD sequences supports a ubiquitous mechanism for pentamer assembly in birnaviruses. The IBDV 452 RR 453 scissile bond (or analogous bonds) is conserved or has (46). Some features of the IBDV T ϭ 13 capsid assembly remain to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

Host Proteolytic Activity Is Necessary for Infectious Bursal Disease Virus Capsid Protein Assembly

Irigoyen

Castón

Rodrı́guez

2012

Journal of Biological Chemistry

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Background: Virus capsid assembly and maturation are regulated by viral and host molecular factors. Results: The puromycin-sensitive aminopeptidase cleaves the capsid protein precursor of a double-stranded RNA virus (IBDV). Conclusion: Puromycin-sensitive aminopeptidase activity is essential for IBDV replication. Significance: Understanding crucial host factors for virus assembly is important for selecting efficient heterologous expression systems and for determining potential antiviral targets.

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Section: Discussionmentioning

confidence: 99%

Host Proteolytic Activity Is Necessary for Infectious Bursal Disease Virus Capsid Protein Assembly

Irigoyen

Castón

Rodrı́guez

2012

Journal of Biological Chemistry

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“…CYV is a bisegmented dsRNA virus from the Entomobirnavirus genus within the Birnaviridae family that was recently isolated from Culex pipiens mosquitoes in Germany (27). Isolation of the highly related Espirito Santo virus and Mosquito X virus in Brazil and China, respectively, indicates that these entomobirnaviruses are widely distributed in nature (28,29). …”

Section: Introductionmentioning

confidence: 99%

Mosquito andDrosophilaentomobirnaviruses suppress dsRNA- and siRNA-induced RNAi

et al. 2014

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RNA interference (RNAi) is a crucial antiviral defense mechanism in insects, including the major mosquito species that transmit important human viruses. To counteract the potent antiviral RNAi pathway, insect viruses encode RNAi suppressors. However, whether mosquito-specific viruses suppress RNAi remains unclear. We therefore set out to study RNAi suppression by Culex Y virus (CYV), a mosquito-specific virus of the Birnaviridae family that was recently isolated from Culex pipiens mosquitoes. We found that the Culex RNAi machinery processes CYV double-stranded RNA (dsRNA) into viral small interfering RNAs (vsiRNAs). Furthermore, we show that RNAi is suppressed in CYV-infected cells and that the viral VP3 protein is responsible for RNAi antagonism. We demonstrate that VP3 can functionally replace B2, the well-characterized RNAi suppressor of Flock House virus. VP3 was found to bind long dsRNA as well as siRNAs and interfered with Dicer-2-mediated cleavage of long dsRNA into siRNAs. Slicing of target RNAs by pre-assembled RNA-induced silencing complexes was not affected by VP3. Finally, we show that the RNAi-suppressive activity of VP3 is conserved in Drosophila X virus, a birnavirus that persistently infects Drosophila cell cultures. Together, our data indicate that mosquito-specific viruses may encode RNAi antagonists to suppress antiviral RNAi.

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“…Six genetic clusters have been identified in the Birnaviridae family [10,11]. Three of them define viruses infecting a unique phylum, the vertebrates: the infectious bursal disease virus (IBDV) infects birds, while the blotched snakehead virus (BSNV), the infectious pancreatic virus (IPNV) and its allies infect fishes.…”

Section: Introductionmentioning

confidence: 99%

“…Three of them define viruses infecting a unique phylum, the vertebrates: the infectious bursal disease virus (IBDV) infects birds, while the blotched snakehead virus (BSNV), the infectious pancreatic virus (IPNV) and its allies infect fishes. The other clusters infect i) insects such as birnaviruses found in dipters, like the Drosophila X virus (DXV), the Drosophila B virus (DBV) and the Esperito Santo virus (ESV) which has been discovered in mosquito cells co-infected with dengue-2 virus [11] and ii) mollusks such as the Tellina virus 1 (TV-1) described from a bivalve [10]. Birnaviruses from rotifers have not been genetically characterized.…”

Section: Introductionmentioning

confidence: 99%

“…A growing number of birnaviruses are characterized in insects [11,17,18], which constitute the main host phylum of the three +ssRNA families ( Noda -, Alphatetra - and Permutotetraviridae ). Taken together, these observations strongly suggest the existence of an ancient virus lineage leading to these last +ssRNA viruses and birnaviruses.…”

Section: Introductionmentioning

confidence: 99%

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Analyses of the radiation of birnaviruses from diverse host phyla and of their evolutionary affinities with other double-stranded RNA and positive strand RNA viruses using robust structure-based multiple sequence alignments and advanced phylogenetic methods

et al. 2013

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BackgroundBirnaviruses form a distinct family of double-stranded RNA viruses infecting animals as different as vertebrates, mollusks, insects and rotifers. With such a wide host range, they constitute a good model for studying the adaptation to the host. Additionally, several lines of evidence link birnaviruses to positive strand RNA viruses and suggest that phylogenetic analyses may provide clues about transition.ResultsWe characterized the genome of a birnavirus from the rotifer Branchionus plicalitis. We used X-ray structures of RNA-dependent RNA polymerases and capsid proteins to obtain multiple structure alignments that allowed us to obtain reliable multiple sequence alignments and we employed “advanced” phylogenetic methods to study the evolutionary relationships between some positive strand and double-stranded RNA viruses. We showed that the rotifer birnavirus genome exhibited an organization remarkably similar to other birnaviruses. As this host was phylogenetically very distant from the other known species targeted by birnaviruses, we revisited the evolutionary pathways within the Birnaviridae family using phylogenetic reconstruction methods. We also applied a number of phylogenetic approaches based on structurally conserved domains/regions of the capsid and RNA-dependent RNA polymerase proteins to study the evolutionary relationships between birnaviruses, other double-stranded RNA viruses and positive strand RNA viruses.ConclusionsWe show that there is a good correlation between the phylogeny of the birnaviruses and that of their hosts at the phylum level using the RNA-dependent RNA polymerase (genomic segment B) on the one hand and a concatenation of the capsid protein, protease and ribonucleoprotein (genomic segment A) on the other hand. This correlation tends to vanish within phyla. The use of advanced phylogenetic methods and robust structure-based multiple sequence alignments allowed us to obtain a more accurate picture (in terms of probability of the tree topologies) of the evolutionary affinities between double-stranded RNA and positive strand RNA viruses. In particular, we were able to show that there exists a good statistical support for the claims that dsRNA viruses are not monophyletic and that viruses with permuted RdRps belong to a common evolution lineage as previously proposed by other groups. We also propose a tree topology with a good statistical support describing the evolutionary relationships between the Picornaviridae, Caliciviridae, Flaviviridae families and a group including the Alphatetraviridae, Nodaviridae, Permutotretraviridae, Birnaviridae, and Cystoviridae families.

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Espirito Santo Virus: a New Birnavirus That Replicates in Insect Cells

Cited by 21 publications

References 48 publications

Host Proteolytic Activity Is Necessary for Infectious Bursal Disease Virus Capsid Protein Assembly

Host Proteolytic Activity Is Necessary for Infectious Bursal Disease Virus Capsid Protein Assembly

Mosquito andDrosophilaentomobirnaviruses suppress dsRNA- and siRNA-induced RNAi

Analyses of the radiation of birnaviruses from diverse host phyla and of their evolutionary affinities with other double-stranded RNA and positive strand RNA viruses using robust structure-based multiple sequence alignments and advanced phylogenetic methods

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