Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering

Beckstead, Benjamin L.; Pan, Sheng; Bhrany, Amit D.; Bratt-Leal, Andrés M.; Ratner, Buddy D.; Giachelli, Cecilia M.

doi:10.1016/j.biomaterials.2005.04.010

Cited by 109 publications

(95 citation statements)

References 34 publications

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“…[39] Only a limited number of studies have attempted to understand epithelial cell interaction to appropriate substrates in vitro; Beckstead et al [40] investigated oesophageal epithelial behavior on a range of synthetic (polyesters) and natural (Alloderm-decellularized skin graft) scaffolds. Alloderm supported the formation of a 5-6 cell layer epithelium with the expression of loricrin (late stage marker for epithelium; similar to involucrin) after 18 d in culture.…”

Section: Discussionmentioning

confidence: 99%

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

Kumar

Workman

O’Brien

et al. 2017

Adv Funct Materials

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Endoscopic treatment of Barrett's oesophagus often leads to further damage of healthy tissue causing fibrotic tissue formation termed as strictures. This study shows that synthetic, self-assembling peptide hydrogels (PeptiGelDesign) support the activity and function of primary oesophageal cells, leading to epithelialization and stratification during in vitro 3D co-culture. Following buffering in culture media, rat oesophageal stromal fibroblasts (rOSFs) are incorporated into a library of peptide hydrogels, whereas mouse oesophageal epithelial cells (mOECs) are seeded on the surface. Optimal hydrogels (PGD-AlphaProC and PGD-CGD2) support mOEC viability (>95%), typical cell morphology (cobblestone-like), and slower migration over a shorter distance compared to a collagen control, at 48 h. Positive expression of typical epithelial markers (ZO-1 and cytokeratins) is detected using immunocytochemistry at day 3 in culture. Furthermore, optimal hydrogels are identified which support rOSF viability (>95%) with homogeneous distribution when incorporated into the hydrogels and also promote the secretion of collagen type I detected using an enzyme linked immunosorbent assay (ELISA), at day 7. A 3D co-culture model using optimal hydrogels for both cell types supports a stratified epithelial layer (expressing involucrin and AE1/AE3 markers). Findings from this study could lead to the use of peptide hydrogels as a minimally invasive endoscopic therapy to manage oesophageal strictures.

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Section: Discussionmentioning

confidence: 99%

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

Kumar

Workman

O’Brien

et al. 2017

Adv Funct Materials

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“…OEC isolation was performed by using the method described by Becksteadet al, 23 with modifications. The buccal mucosa biopsy was weighed and immersed for a few seconds in an iodide solution diluted to half with 1Xphosphate-buffered saline (PBS) (Invitrogen), and then rinsed two times in 1XPBS.…”

Section: Oecs Isolation and Characterizationmentioning

confidence: 99%

“…Two comparative works, 23,47 which used epithelial cells and scaffolds of different origins, state that the combination of native esophageal squamous cells with a natural scaffold brings the best results in terms of proliferation of the basal layer, epithelial stratification, and cell adhesion.…”

Section: Cells Cultured In Three Different Conditions (On Sis In Petrimentioning

confidence: 99%

“…Some authors cultivate squamous epithelial cells in the presence of the feeder cells (lethally irradiated mouse fibroblasts), 17,47 while others performed their culture without. 16,23 Although we did not use these cells, we obtained a pluristratified epithelium with a basal layer of proliferative cells.…”

Section: Cells Cultured In Three Different Conditions (On Sis In Petrimentioning

confidence: 99%

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In Vitro Development and Characterization of a Tissue-Engineered Conduit Resembling Esophageal Wall Using Human and Pig Skeletal Myoblast, Oral Epithelial Cells, and Biologic Scaffolds

Poghosyan

Gaujoux

Vanneaux

et al. 2013

Tissue Engineering Part A

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Introduction: Tissue engineering represents a promising approach for esophageal replacement, considering the complexity and drawbacks of conventional techniques. Aim: To create the components necessary to reconstruct in vitro or in vivo an esophageal wall, we analyzed the feasibility and the optimal conditions of human and pig skeletal myoblast (HSM and PSM) and porcine oral epithelial cell (OEC) culture on biologic scaffolds. Materials and Methods: PSM and HSM were isolated from striated muscle and porcine OECs were extracted from oral mucosa biopsies. Myoblasts were seeded on an acellular scaffold issue from porcine small intestinal submucosa (SIS) and OEC on decellularized human amniotic membrane (HAM). Seeding conditions (cell concentrations [0.5 · 10 6 versus 10 6 cells/cm 2 ] and culture periods [7, 14 and 21 days]), were analyzed using the methyl thiazoltetrazolium assay, quantitative PCR, flow cytometry, and immunohistochemistry. Results: Phenotypic stability was observed after cellular expansion for PSM and HSM (85% and 97% CD56-positive cells, respectively), and OECs (90% AE1/AE3-positive cells). After PSM and HSM seeding, quantities of viable cells were similar whatever the initial cell concentration used and remained stable at all time points. During cell culture on SIS, a decrease of CD56-positive cells was observed (76% and 76% by D7, 56% and 70% by D14, 28% and 60% by D21, for PSM and HSM, respectively). Multilayered surface of a-actin smooth muscle and Desmine-positive cells organized in bundles was seen as soon as D7, with no evidence of cell within the SIS. Myoblasts fusion was observed at D21. Pax3 and Pax7 expression was downregulated and MyoD expression upregulated, at D14.OEC proliferation was observed on HAM with both cell concentrations from D7 to D21. The cell metabolism activity was more important on matrix seeded by 10 6 cells/cm 2 . With 0.5 · 10 6 OEC/cm 2

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“…The se bi odeg ra dab le poly mers hold gre at pro mi se for overco ming the chal len ge of li mi ted cell so ur ces for tis su e re pa ir as scaf folds and su tu res as well as for drug pe net ra ti on as mic ro o cap su les. [1][2][3][4] Pre vi o us re ports ha ve shown that mul ti cel lular tu mor sphe ro ids (MTS) rep re sent an in ter me diary le vel bet we en mo no la yer gro wing cells and tu mors in ani mals and hu mans. The sphe ro ids more clo sely mi mic the re al bi o lo gic en vi ron ment by pro vi ding cell-to-cell ad he si on and sig na ling.…”

mentioning

confidence: 99%

Evaluation and Comparison of In Vitro Biocompatibility of Poly (Glycolic Acid) and Poly (Lactide-Co-Glycolide Acid) on Mature Spheroids of Tumorigenic and Non-Tumorigenic Cell Lines

Bılır¹,

Ergüven²,

Zilan³

et al. 2011

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : The aim of this study was to eva lu a te and com pa re in vit ro bi o com pa ti bi lity and poly(glyco lic acid) (PGA) and poly(lac ti de-co-glyco li de acid) (PLGA) on tu mo ri ge nic and non-tu mo ri genic ma tu re sphe ro ids. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : This is an in vit ro ex pe ri men tal study. Tu mo ri ge nic (C6 glio ma, SH-SY5Y, MDAH2774, MCF-7) and non-tu mo ri ge nic cells [CRL11372, pri mary os te ob lasts (MCPO)] as well as the ir ma tu re sphe ro ids we re cul tu red alo ne as a con trol gro up as well as in combination with PGA and PLGA. To tal cell num bers, bro mo de ox yu ri di ne la be ling in dex (BrDU-LI), apop to sis, morp ho logy, and ul tras truc tu re we re eva lu a ted. R Re e s su ul lt ts s: : PGA and PLGA sig ni fi cantly dec re a sed the number of SH-SY5Y and C6 gli o ma cells; MDAH 2774 cells al so dec re a sed, but not sig ni fi cantly (p> 0.05). Low BrDU-LI (p< 0.05) with a high le vel of apop to sis (p< 0.05) at C6 gli o ma and a high le vel of BrDU-LI (p< 0.05) with a low le vel of apop to sis at MDAH2774 (p< 0.01) we re noted. The se bi o poly mers mostly dec re a sed the num ber of CRL-11372 cells (p< 0.05), but in di ca ted an in cre a sed apop to sis (p< 0.01) and sig ni fi cant BrDU-LI (p< 0.05). Bi o poly mers in du ced chro ma tin con den sa ti on (typi cal apop to tic ul tras truc tu re) and va cu oli za ti on pri ma rily at SH-SY5Y sphe ro ids but ra rely at MDAH-2774 sphe ro ids. This apop to tic ul tras tuc tu re was most of ten ob ser ved at MCPO sphe ro ids. PLGA and PGA in du ced si mi lar BrDU-LI dec re a ses among tu mo ri ge nic sphe ro ids (p< 0.05), alt ho ugh this dec re a se was gre a ter at MCF-7 (p< 0.05) in the PGA group. PGA pri ma rily dec re a sed BrDU-LI at CRL 11372 (p< 0.05), alt ho ugh the dec re a se was al most iden tical to that at MCPO for the two bi o poly mers (p< 0.05). A sig ni fi cant at tach ment af fi nity was de ter mi ned at MDAH -2774 and C6 gli o ma sphe ro ids. C Co on nc c l lu u s si i o on n: : This study de mons tra ted the bi o com pa ti bi lity of PGA and PLGA at ma tu re sphe ro ids of tu mo ri ge nic and non-tu mo ri ge nic cell li nes, which chan ged accor ding to the cell type.K Ke ey y W Wo or rd ds s: : Polyg lyco lic acid; ma te ri als tes ting; sphe ro ids, cel lu lar Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da, po li(gli ko lik asit) (PGA) ve po li(lak tid-ko-gli ko lid) (PLGA)'in tü mö ri je nik ve non-tü mö ri je nik ma tür sfe ro id ler de bi yo u yum lu lu ğu nun de ğer len di ril me si ve kar şı laş tı rıl ma sı amaç landı. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Ça lış ma de ney sel ola rak plan lan dı. Tü mö ro je nik (C6 gli o ma, SH-SY5Y, MDAH 2774, MCF-7) ve non-tü mö ro je nik [CRL 11372, pri mer os te ob last (MCPO)] hüc re ler ve ol gun sfe ro id le ri tek ba şı na ve bi yo po li mer ler ile bir lik te kül tü re edil di. Top lam hüc re sa yı sı, bro mo de ok si u ri din işa ret leme in dek s...

show abstract

Esophageal epithelial cell interaction with synthetic and natural scaffolds for tissue engineering

Cited by 109 publications

References 34 publications

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

In Vitro Development and Characterization of a Tissue-Engineered Conduit Resembling Esophageal Wall Using Human and Pig Skeletal Myoblast, Oral Epithelial Cells, and Biologic Scaffolds

Evaluation and Comparison of In Vitro Biocompatibility of Poly (Glycolic Acid) and Poly (Lactide-Co-Glycolide Acid) on Mature Spheroids of Tumorigenic and Non-Tumorigenic Cell Lines

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