Escherichia coli hyperepidemic clone ST410-A harboring blaCTX-M-15 isolated from fresh vegetables in a municipal market in Quito-Ecuador

Ortega-Paredes, David; Barba, Pedro; Mena-López, Santiago; Espinel, Nathaly; Zurita, Jeannete

doi:10.1016/j.ijfoodmicro.2018.04.037

Cited by 34 publications

(24 citation statements)

References 21 publications

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“…E. coli ‐ST410 has been identified as a new pandemic clone (Schaufler et al ), and it was previously reported in vegetable samples (Ortega‐Paredes et al ) and clinical samples (Zurita et al ) in Ecuador. E. coli ‐ST410 has been isolated from animal (Zogg et al ) and environmental samples, suggesting that this clone plays a relevant role in the human–animal–environment interface proposed by the ‘One Health’ concept (Wielinga and Schlundt ).…”

Section: Resultsmentioning

confidence: 89%

“…Phylogenetic groups A (8/20; 40%) and B1 (6/20; 30%) were the most common, followed by group D (3/20; 15%) and group B2 (3/20; 15%; Table ). Recent studies have also been characterizing A and B1 E. coli strains frequently from different types of food samples in Ecuador (Ortega‐Paredes et al ). Therefore, E. coli belonging to phylogenetic groups A and B1 could be a possible transmission route to humans through the food supply.…”

Section: Resultsmentioning

confidence: 99%

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Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Zurita

Yánez

Sevillano³

et al. 2020

Lett Appl Microbiol

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Significance and Impact of the Study: This study identified ESBL-producing Escherichia coli epidemic clones: ST131, ST410 and ST744 in ready-to-eat street food samples. Street food is a possible way to spread harm multidrug-resistant (MDR) E. coli strains in the community. Studies to identify the contamination sources of this kind of food are needed to tackle MDR E. coli dissemination. AbstractReady-to-eat food contamination with ESBL-producing Escherichia coli is a growing health concern. Some of these strains also are epidemic clones and can cause community-associated infections that are difficult to treat. In this study, the occurrence of ESBL-producing E. coli contaminated ready-to-eat street food in Quito, Ecuador was evaluated. In total, 150 samples were collected randomly in the most crowded sites of the city. In all, 34 samples (34/150; 22Á6%) were positive for total thermotolerant (44Á5°C) coliforms resistant to cefotaxime. MALDI-TOF analysis identified that the E. coli was found in 20 food samples (20/34; 59%). ESBL gene bla CTX-M-55 was identified in nine isolates, bla CTX-M-15 in six isolates, bla CTX-M-14 in two isolates, and one isolate each harboured bla CTX-M-24 , bla CTX-M-65 , bla CTX-M-55 and bla CTX-M-8 . Phylogenetic groups like A and B1 were the most common, followed by groups D and B2. MLST analysis identified 12 different sequence types (STs), the most common was ST162. Recognized epidemic clonal groups ST410, ST131 and ST744 were encountered. Ready-to-eat street food is a potential way of spreading ESBL-producing E. coli epidemic clones in Quito, Ecuador.Letters in Applied Microbiology ISSN 0266-8254

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Zurita

Yánez

Sevillano³

et al. 2020

Lett Appl Microbiol

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“…Likewise, antimicrobial resistance can readily spread to humans through the vegetables contaminated with resistant bacteria. ESBL-ECs have also been found in vegetables, principally ready-to-eat vegetables 19,25 and raw vegetables [28][29][30][31][32] . In this study, we isolated the cefotaxime-resistant ESBL-ECs from eleven (0.83%) of 1324 raw vegetables, indicating a lower Figure 1.…”

Section: Discussionmentioning

confidence: 99%

“…Despite the small number of E. coli isolates from different types of vegetables, the ESBL genotypes have been reported to be geographically distinct. The main types are bla CTX-M-15 in the Dominican Republic 30 , India 30 , and Ecuador 28 , bla CTX-M-14 in South Africa 31 , bla CTX-M-55 in Thailand 30 , and bla CTX-M-65 in Vietnam 30 . In South Korea, bla CTX-M-55 and bla CTX-M-14 were found in E. coli isolates from readyto-eat sprouts between 2012 and 2013 25 , and bla CTX-M-55 was also detected in the colistin-resistant E. coli ST10 www.nature.com/scientificreports/ from lettuce in 2018 44 .…”

Section: Discussionmentioning

confidence: 99%

“…To the best of our knowledge, this is the first report of ESBL-EC ST354 in vegetables. Furthermore, multiple ESBL-ExPEC clones, including ST131, ST405, ST410, and ST393, have been detected in raw vegetables 28,30 . These results suggest the potential roles of vegetables as the reservoir and propagator for ESBL-ExPEC lineages, which increases the threat of human health as a result of direct consumption.…”

Section: Discussionmentioning

confidence: 99%

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Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

Song

Kim

et al. 2020

Sci Rep

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The increasing prevalence of oxyimino-cephalosporin-resistant Enterobacteriaceae has become a global concern because of their clinical impact on both human and veterinary medicine. The present study determined the prevalence, antimicrobial susceptibility, and molecular genetic features of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) isolates from raw vegetables. A total of 1324 samples were collected from two agricultural wholesale markets in Incheon, South Korea in 2018. The ESBL-EC strains were isolated from 0.83% (11/1324) samples, and all of them were resistant to ampicillin, piperacillin, cefazoline, cefotaxime, and nalidixic acid and yielded CTX-M-type ESBL, including CTX-M-14, CTX-M-15, CTX-M-55, CTX-M-27, and CTX-M-65. The isolates belonged to phylogenetic subgroups D (n = 5), A (n = 4), and B1 (n = 2). Multilocus sequence typing revealed nine known E. coli sequence types (STs), including ST10, ST38, ST69, ST101, ST224, ST349, ST354, ST2509, ST2847, and two new STs. Notably, ST69, ST10, ST38, and ST354 belong to the major human-associated extraintestinal pathogenic E. coli lineages. Our results demonstrate that ESBL-producing multidrug-resistant pathogens may be transmitted to humans through the vegetable intake, highlighting the importance of resistance monitoring and intervention in the One Health perspective.

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WHO Critical Priority Escherichia coli in Latin America: A One Health Challenge for a Post-Pandemic World

Lincopán

Fuentes‐Castillo

Espinoza-Muñoz³

et al. 2023

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Escherichia coli hyperepidemic clone ST410-A harboring blaCTX-M-15 isolated from fresh vegetables in a municipal market in Quito-Ecuador

Cited by 34 publications

References 21 publications

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Ready‐to‐eat street food: a potential source for dissemination of multidrug‐resistant Escherichia coli epidemic clones in Quito, Ecuador

Extended-spectrum β-lactamase-producing Escherichia coli isolated from raw vegetables in South Korea

WHO Critical Priority Escherichia coli in Latin America: A One Health Challenge for a Post-Pandemic World

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