1969
DOI: 10.1021/bi00839a041
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Abstract: and the strate affinities and nonlinear kinetics were observed when K+ was in low concentration or absent from the reaction mixture. The pH optimum of the enzyme was 8.0. The molecular weight, estimated by gel filtration, was 280,000 daltons, and was independent of the concentration of potassium in the eluent. A shift in the ultraviolet absorption spectrum was observed when potassium was added to the salt-free enzyme.

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Cited by 99 publications
(11 citation statements)
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References 16 publications
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“…Other enzymatic assays Activity of the citrate synthase was determined according to Faloona and Srere (1969). Aconitase and fumarase activities were determined according to Gardner et al (1994), with the modification that the extraction medium was supplemented with tri-sodium citrate to stabilize aconitase activity ex vivo.…”
Section: Measurement Of Respiratory Chain Complex Activitiesmentioning
confidence: 99%
“…Other enzymatic assays Activity of the citrate synthase was determined according to Faloona and Srere (1969). Aconitase and fumarase activities were determined according to Gardner et al (1994), with the modification that the extraction medium was supplemented with tri-sodium citrate to stabilize aconitase activity ex vivo.…”
Section: Measurement Of Respiratory Chain Complex Activitiesmentioning
confidence: 99%
“…Citrate synthase was assayed by using a modification of the method described previously (16,17). Cultures were grown in 1-liter flasks containing 250 ml of Luria broth for 16 h at 35°C and 150 rpm.…”
Section: Nad(p)h/nad(p)mentioning
confidence: 99%
“…The ratio of NAD(P)H to NAD(P) ϩ has been shown to alter cellular patterns of metabolic flux (14). NAD(P)H is an allosteric inhibitor of many enzymes, including pyruvate dehydrogenase (20), phosphotransacetylase (41), malate dehydrogenase (38), and citrate synthase (17,48). In KO11, addition of acetaldehyde or pyruvate (metabolized to acetaldehyde by recombinant pyruvate decarboxylase) would be expected to decrease the level of NAD(P)H and the NAD(P)H/NAD(P) ϩ ratio by increasing the pool of acetaldehyde available for reduction to ethanol.…”
Section: Vol 68 2002 Flux Through Citrate Synthase Limits Growth 1075mentioning
confidence: 99%
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“…Glycolytic flux was calculated as the sum of lactate and pyruvate released into the coronary effluent. Citrate-synthase activity was determined [43] in myocardium to estimate mitochondrial density. Aconitase and fumarase activities were determined in homogenates of the reperfused myocardium, according to the method of Gardner et al [44].…”
Section: Biochemical Analysesmentioning
confidence: 99%