Escherichia coli as a Pathogen∗

Smith, James L.; Fratamico, Pina M.

doi:10.1016/b978-0-12-385007-2.00007-3

Cited by 12 publications

(12 citation statements)

References 8 publications

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“…These results come in line with (22) and lower than that reported in Iran (24).EPEC not detected in 8˃ years age group and this may a ributed to the development of immunity or the loss of receptors for some specific adhesion molecules (27).The EPEC serogroups come in line with that detected in other studies (26). EPEC commonly transmitted via the fecal-oral route in a poor hygienic environment (28) . Results of studies carried out in areas with different health and sociodemographic characteristics showed that strains of EPEC were the major cause of diarrhea in developing countries (29).…”

Section: Discussionsupporting

confidence: 88%

Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender

2020

GMJ

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Background and Objectives:The aim is to determine the role of Enteropathogenic Escherichia coli (EPEC) serotypes (O111, O55, O26, O86, O119, O127, O114, and O142) in C-reactive protein (CRP) level in acute diarrhea among children; to determine the possible effect of age and gender in CRP level. Methods: Culture of stool samples from 64 children in MacConkey agar for primary isolation of E.coli and confirmed via API20E . Sero-grouping of E.coli performed via specific antisera . CRP detected by latex agglutination. Results: In this study, 64 children with diarrhea were involved. The mean age was 2.92 years. Males represent 34/64, 53.1%. The range of CRP level 12-96 mg/dl. EPEC1 and EPEC3 not detected. EPEC2 detected among 14/64, 21.9% and EPEC4 in 18.8%. Among the age group ˂1 year, EPEC2 detected in 9.38%, with mean CRP level 12mg/dl; 3.13%, 48 mg/dl 6.25% . EPEC4 detected in 2/64, 3.13%, with mean CRP level 12mg/dl. Age group 1-4 year, EPEC2 detected in 6/64 , 9.38% , with mean CRP level 24mg/dl. EPEC4 detected in 6/64, 9.38%; with mean CRP level 12mg/dl, 24mg/dl, 48mg/dl 3.13% in each. Among the age group 4.1-8 year, EPEC4 detected in 4/64 ,6.25% , with CRP mean level 48 mg/dl. EPEC not detected in 8˃ years age group .EPEC2 detected in male 28/64 , 43.75% and the CRP level range from 12-96mg/dl .EPEC2 detected in 22/64 females, 34.38% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level detected. EPEC4 detected in males 26/64 , 40.63% , CRP level range from 12-96mg/dl . EPEC4 detected in 26/64 females , 40.63% , CRP level range from 12-96mg/dl . No significant difference or correlation between gender and CRP level was detected. EPEC2 and CRP level significantly correlated with patients age . while infection with EPEC2 inversely correlated EPEC4. No significant correlation between gender and EPEC serotypes. Conclusion: Although EPEC2 and EPEC4 frequently detected in diarrhea cases of 1-8 years old, only EPEC2 positively correlated with age. Even with a fluctuation in CRP level, it was positively correlated with age and not correlated with EPEC serotypes and gender.

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Section: Discussionsupporting

confidence: 88%

Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender

2020

GMJ

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“…In addition to shiga toxin, an external membrane protein called Intimin is responsible for the close connection of the bacteria to the intestinal epithelial cells and causes certain damage, called "effacing/attaching," and is coded by the eae gene [3,4]. Also, the enterohemolysin, coded by the hly gene, is an effective factor in the pathogenicity of this serotype [5].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Shiga toxin-producing Escherichia coli isolated from chicken meat in west of Iran

Zarei¹,

Shokoohizadeh²,

Hossainpour³

et al. 2019

Preprint

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Objective: Shiga toxin producing Escherichia coli (STEC) has known as a crucial zoonotic food borne pathogen. A total of 257 row chicken meat samples were collected from different markets in Hamadan city from January 2016 to May 2017. Samples were cultured on selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by disk diffusion method. The genetic relatedness of STEC isolates were analyzed by ERIC-PCR. Results: Totally, 93(36%) of isolates were identified as E. coli in this current study. According serological and microbiological tests, 5(5.3%), 31(33.3%) and 7(7.5%) of E. coli isolates, characterized as Enterohemorrhagic E. coli (EHEC), STEC and attaching and effacing E. coli (AEEC) strains, respectively. High level resistance to tetracycline (89.8), ampicillin (82.8%) and sulfametoxazole-trimotoprime (71%) were detected among E. coli isolates. Analysis of ERIC-PCR results showed five different ERIC types among EHEC isolates. Based on our findings, chicken meat identified as a sources of STEC strains, therefore, the controlling and checkup the chicken meats for the resistance and virulent strains of E. coli should be consider as a crucial issues in public health.

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“…In addition to Shiga toxins, an external membrane protein called intimin is responsible for the attachment of bacteria to the intestinal epithelial cells, causes a certain damage (attaching-effacing lesions (A/E)), and is encoded by the eae gene [3,4]. Also, enterohemolysin, encoded by the hly gene, is an effective factor in the pathogenicity of STEC [5]. Because only limited and incomplete studies have been conducted on the prevalence and epidemiology of the O157:H7 serotype in developing countries, its prevalence has been reported as low [6,7].…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Shiga toxin-producing and Enteropathogenic Escherichia coli Isolated from Chicken Meat in the west of Iran

Zarei

Shokoohizadeh

Hossainpour

et al. 2019

Preprint

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Objective: Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic foodborne pathogen. Totally, 257 raw chicken meat were collected from markets in Hamadan, west of Iran. The samples were cultured on selective media and the virulence genes of E. coli isolates were analyzed by PCR. The antibiotic resistance patterns were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results: Totally, 93 (36%; 95% CI 41.9- 30.1%) isolates were identified as E. coli. Based on microbiological tests, 36 (38.7%; 95% CI 48.6-28.8), 7 (7.5%; 95% CI 12.8-2.2%), and 12 (12.9%; 95% CI 19.7- 6.1%) of the E. coli isolates were characterized as STEC, Enteropathogenic E. coli, and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4%; 95% CI 97.1- 85.7%), tetracycline (89.8%; 95% CI 96.2-83.5%), ampicillin (82.8%; 95% CI 90.2-75.1%), and sulfametoxazole-trimotoprime (71%; 95% CI 80.2-61.8%) was detected among the E. coli isolates. The analysis of ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Based on findings. Control and check-up of poultry meats should be considered as a crucial issue for public health.

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Escherichia coli as a Pathogen∗

Cited by 12 publications

References 8 publications

Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender

Evaluation of C-Reactive Protein in Iraqi Children Presented with Acute Enteropathogenic Escherichia Coli Associated Diarrhea with Special Emphasis to Age and Gender

Prevalence of Shiga toxin-producing Escherichia coli isolated from chicken meat in west of Iran

Prevalence of Shiga toxin-producing and Enteropathogenic Escherichia coli Isolated from Chicken Meat in the west of Iran

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