ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in poultry in the Czech Republic

Kolář, Milan; Bardoň, Jan; Chromá, M; Hricová, Kristýna; Štosová, Taťána; Šauer, Pavel; Koukalová, Dagmar

doi:10.17221/165/2009-vetmed

Cited by 31 publications

(23 citation statements)

References 24 publications

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“…The prevalence was lower in comparison to those detected by Smet et al [11] 43% and Kolar et al [20] 25.9%. On the other hand, Blanc et al [23] reported the prevalence rate of E. coli isolates as AmpC β-lactamase producers was 6.7%.…”

Section: Discussioncontrasting

confidence: 73%

“…On the contrary to these results, Kolar et al [20] described the most frequent ESBL types were SHV. In Spain, SHV-12-producing E. coli strains were then sporadically recovered in faecal samples from healthy chickens [24] .…”

Section: Discussionmentioning

confidence: 67%

“…Unfortunately, for this reason, plasmid-mediated AmpC β-lactamase resistance goes undetected in most clinical laboratories [20] . Also, some researchers stated that two or more of β-lactamase resistance genes can be found in the frequently isolated Gram-negative bacteria [33] .…”

Section: Discussionmentioning

confidence: 99%

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Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Kahraman¹,

Sığırcı²,

Çelik³

et al. 2016

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the presence of Extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase producing Escherichia coli strains isolated from meat and faecal samples of chicken. Faecal samples (n: 384) were collected from broiler and egg-type healthy chickens in 43 flocks and 384 fresh chicken meat samples from local markets, supermarkets and slaughterhouses. E. coli isolates were tested phenotypically according to the guidelines of CLSI (2013) for ESBL production. Phenotypic detection of AmpC production was carried out by determination of resistance to cefoxitin and susceptibility to cefepime. Also, presence of genes encoding different types of β-lactamases (blaCTX-M, blaTEM, blaSHV and the mutation in the promoter of AmpC) in all phenotypically ESBL or AmpC producing E. coli was investigated by PCR assays. In this study, blaCTX-M (95.4%), blaCTX-M-1 (81.8%), blaCTX-M-8 (4.5%), blaTEM (45.4%) and blaSHV (2.2%) genes were detected from faecal samples and blaCTX-M (100%), blaCTX-M-1 (28.5%) and blaTEM (7.1%) from meat samples. The only plasmidic AmpC β-lactamase found was the CIT type. Keywords: Escherichia coli, ESBL, AmpC, Chicken, PCR Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz üreten Escherichia coli İzolatlarının Saptanması ÖzetBu çalışmanın amacı, tavuk eti ve dışkı örneklerinden izole edilen Genişlemiş Spektrumlu β-laktamaz (GSBL) ve AmpC β-laktamaz üreten Escherichia coli izolatlarının varlığını ortaya koymaktır. Bu çalışmada, 43 kümesten sağlıklı tavuklara ait 384 dışkı örneği ve yerel marketlerden, süpermarketlerden ve kesimhanelerden 384 taze tavuk eti örneği toplandı. E. coli izolatları fenotipik olarak CLSI (2013) standartları kullanılarak GSBL üretimi yönünden incelendi. AmpC üretiminin fenotipik olarak saptanması sefoksitine direnç ve sefepime duyarlılık kriterleri ile belirlendi. Fenotipik olarak GSBL ya da AmpC üreten E. coli olarak saptanan izolatlarının hepsi PCR ile değişik tipteki β-laktamaz genlerinin (blaCTX-M, blaTEM, blaSHV, AmpC) varlığını ortaya koymak için incelendi. Bu çalışmada, dışkı örneklerinde blaCTX-M (%95.4), blaCTX-M-1 (%81.8), blaCTX-M-8 (%4.5), blaTEM (%45.4), blaSHV (%2.2) genleri ve tavuk eti örneklerinde ise blaCTX-M (%100), blaCTX-M-1 (%28.5), blaTEM (%7.1) genleri saptandı. AmpC β-laktamaz grubunda sadece CIT geni belirlendi.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 67%

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Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Kahraman¹,

Sığırcı²,

Çelik³

et al. 2016

Kafkas Univ Vet Fak Derg

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“…Percentages of samples of food-producing animals or food in which ESBL-carrying E. coli isolates were detected varied from 0.2% to 40.1%, according to the country and the methodology used [ (Smet et al, 2009); Table 6]. Occurrences in the range of 10-40% were found when healthy poultry or pigs were analysed for the presence of ESBL-positive E. coli isolates in Portugal (Costa et al, 2009;Goncalves et al, 2010;Machado et al, 2008), Netherlands (Dierikx et al, 2010a), France (Girlich et al, 2007), and slightly lower percentages were identified in Czech Republic (Kolar et al, 2010). Moreover, ESBL-carrying E. coli isolates were found in most of pig and poultry farms tested in Spain , and also in five poultry farms tested in Italy (Bortolaia et al, 2010b).…”

Section: Prevalence Of Esbl-producing E Coli and Salmonella In Food-mentioning

confidence: 99%

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

2011

EFSA Journal

240

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The potential contribution of food-producing animals or foods to public health risks by ESBL and/or AmpCproducing bacteria is related to specific plasmid-mediated ESBL and/or AmpC genes encoded by a number of organisms. The predominant ESBL families encountered are CTX-M, TEM, and SHV; the predominant AmpCfamily is CMY. The most common genes associated with this resistance in animals are bla CTX-M-1 (the most commonly identified ESBL), and bla CTX-M-14 , followed by bla and bla Among the genes encoding AmpC-type β-lactamases, bla CMY-2 is the most common.The bacterial species most commonly identified with these genes are Escherichia coli and non-typhoidal Salmonella. ESBL/AmpC transmission is mainly driven by integrons, insertion sequences, transposons and plasmids, some of which are homologous in isolates from both food-production animals and humans. Cefotaxime is used as the drug of choice for optimum detection of bla ESBL and/or bla AmpC genes. The preferred method for isolation of ESBL-and/or AmpC-producers is screening on selective agar preceded by selective enrichment in a broth.The establishment of risk factors for occurrence of ESBL/AmpC-producing bacteria is particularly complicated by the data unavailability or lack of its accuracy. The use of antimicrobials is a risk factor for the selection and spread of resistant clones, resistance genes and plasmids. Since most ESBL-and AmpC-producing strains carry additional resistances to other commonly-used veterinary drugs, generic antimicrobial use is a risk factor for ESBL/AmpC and it is not restricted specifically to the use of cephalosporins. An additional risk factor is extensive trade of animals in EU MS. There are no data on the comparative efficiency of individual control options in reducing public health risks caused by ESBL and/or AmpC-producing bacteria related to food-producing animals. Prioritisation is complex, but it is considered that a highly effective control option would be to stop all uses of cephalosporins/systemically active 3 rd /4 th generation cephalosporins, or to restrict their use (use only allowed under specific circumstances). As co-resistance is an important issue, it is also of high priority to decrease the total antimicrobial use in animal production in the EU. KEY WORDSResistance, ESBLs, AmpC, occurrence, transmission, control options, public health microbiology. SUMMARYFollowing a request from the European Commission, the Panel on Biological Hazards (BIOHAZ) was asked to deliver a Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum beta (β)-lactamases (ESBL) and/or AmpC β-lactamases (AmpC) in food and foodproducing animals. In particular, the Panel was asked: (i) to propose a definition of the ESBL-and/or AmpC-producing bacterial strains and genes relevant for public health and linked to food-producing animals or food borne transmission; (ii) to review the information on the epidemiology of acquired resistance to broad spectrum cephalosporins including the genes coding for ...

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“…Occurrence in the range of 10%-40% was found when healthy poultry or pigs were analysed for the presence of ESBL-positive E. coli isolates in Portugal (17), the Netherlands (7), France (11), and slightly lower percentages were identified in the Czech Republic (15). The recent data from Turkey shows that in 13.4% of E. coli isolated from meat (poultry and beef) ESBL production was confirmed by the phenotypic tests (1).…”

Section: Discussionmentioning

confidence: 99%

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Wasiński

Różańska

Osek

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was a preliminary determination of occurrence of extended spectrum β-lactamases (ESBL)-and AmpC-producing Escherichia coli (E.coli) in raw meat samples collected from slaughter-houses located in different regions of Poland. A total of 141 samples were tested, comprising 78 pork samples, 44 beef samples, and 19 chicken meat samples. Isolated and identified E. coli strains were examined for the ESBL and/or AmpC β-lactamases production by the use of four disc diffusion and minimum inhibitory concentration tests. All strains positive in one or both tests were examined by PCR for the presence of the bla CTX , bla TEM , bla SHV , and bla CMY-2 group genes. During the study, 154 E. coli strains were isolated from 95 samples. Among these, 18 (11.7%) strains were identified in phenotypic tests as ESBL-producing and seven (4.5%) strains as AmpC-positive. The presence of the genes encoding selected ESBL-s (TEM, CTX, SHV) was identified in 14 of the strains recognised as ESBLpositive in phenotypic tests. All AmpC-positive isolates showed the presence of the CMY-2 group encoding genes. One of these strains had also the CTX-M and TEM genes, and four of them expressed the TEM marker.

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ESBL and AmpC beta-lactamase-producing Enterobacteriaceae in poultry in the Czech Republic

Cited by 31 publications

References 24 publications

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Tavuklarda Genişlemiş Spektrumlu β-laktamaz ve AmpC β-laktamaz Üreten Escherichia coli İzolatlarının Saptanması

Scientific Opinion on the public health risks of bacterial strains producing extended-spectrum β-lactamases and/or AmpC β-lactamases in food and food-producing animals

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

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