ES cell technology: An introduction to genetic manipulation, differentiation and therapeutic cloning

Hook, Lilian; O'Brien, Carmel; Allsopp, Timothy E.

doi:10.1016/j.addr.2005.07.018

Cited by 13 publications

(7 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the past decade, ESCs have also been obtained from nonhuman primate and human embryos (Thomson and Kalishman, 1998;Sasaki et al, 2005;Suemori et al, 2001). Human ES cells (hESCs) are potentially valuable for the provision of therapeutic cells whose growth and differentiation can be controlled for the treatment of disease (Hook et al, 2005;Liew et al, 2005). However, for ESC-based therapy to become a clinical reality, translational research involving nonhuman primates is essential because of the many regulations and ethical considerations surrounding the use of hESCs.…”

Section: Introductionmentioning

confidence: 99%

Primate Embryonic Stem Cells Proceed to Early GametogenesisIn Vitro

Teramura

Takehara

Kawata

et al. 2007

Cloning and Stem Cells

View full text Add to dashboard Cite

Embryonic stem cells (ESCs) of nonhuman primates are important for research into human gametogenesis because of similarities between the embryos and fetuses of nonhuman primates and those of humans. Recently, the formation of germ cells from mouse ESCs in vitro has been reported. In this study, we established cynomolgus monkey ES cell lines (cyESCs) and attempted to induce their differentiation into germ cells to obtain further information on the development of primate germ cells by observing the markers specific to germ cells. Three cyESCs were newly established and confirmed to be pluripotent. When the cells are induced to differentiate, the transcripts of Vasa and some meiotic markers were expressed. VASA protein accumulated in differentiated cell clumps and VASA-positive cells gathered in clumps as the number of differentiation days increased. In the later stages, VASA-positive clumps coexpressed OCT-4, suggesting that these cells might correspond to early gonocytes at the postmigration stage. Furthermore, meiosis-specific gene expression was also observed. These results demonstrate that cyESCs can differentiate to developing germ cells such as primordial germ cells (PGCs) or more developed gonocytes in our differentiation systems, and may be a suitable model for studying the mechanisms of primate germ cell development.

show abstract

Section: Introductionmentioning

confidence: 99%

Primate Embryonic Stem Cells Proceed to Early GametogenesisIn Vitro

Teramura

Takehara

Kawata

et al. 2007

Cloning and Stem Cells

View full text Add to dashboard Cite

show abstract

“…Undifferentiated ES cells are frequently regarded as a potential starting material for regenerative medicine applications or for biological, pharmacological and toxicological studies in vitro as a reliable alternative to animal models (Hook et al 2005;Pouton and Haynes 2005). Therefore, a deeper understanding of the factors affecting ES cell expansion and/or controlled differentiation is needed in order to develop scale-up strategies to produce a significant number of cells for such applications.…”

Section: Introductionmentioning

confidence: 99%

Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions

Fernandes

Fernandes‐Platzgummer

Silva

et al. 2009

Biotechnol Lett

View full text Add to dashboard Cite

There is a need for a deeper understanding of the biochemical events affecting embryonic stem (ES) cell culture by analyzing the expansion of mouse ES cells in terms of both cell growth and metabolic kinetics. The influence of the initial cell density on cell expansion was assessed. Concomitantly, the biochemical profile of the culture was evaluated, which allowed measuring the consumption of important substrates, such as glucose and glutamine, and the production of metabolic byproducts, like lactate. The results suggest a more efficient cell metabolism in serum-free conditions and a preferential use of glutaminolysis as an energy source during cell expansion at low seeding densities. This work contributes to the development of fully-controlled bioprocesses to produce relevant numbers of ES cells for cell therapies and high-throughput drug screening.

show abstract

“…High-resolution genetic and physical linkage maps of the mouse genome have greatly facilitated the identification and cloning of mouse disease genes. Furthermore, transgenic approaches allowed us to ectopically express or make germline mutations in virtually any gene in the mouse genome by using homologous recombination in embryonic stem (ES) cells [2, 3]. Inbred, congenic and transgenic strains are widely used in current research labs as very valuable tools to investigate human diseases pathogenesis and develop new effective therapeutical strategies.…”

Section: Human Disease Mouse Modelsmentioning

confidence: 99%

“…Homologous recombination between genomic and the exogenous DNA is a very inefficient and rare process, but it takes place in ES cells with relatively higher efficiency than it does in other cell types [4]. Gene targeting by homologous recombination in ES cells has improved our ability to study many biological processes [3]. Since ES cells contribute to all tissues upon injection into a recipient blastocyst, including the germline [6, 7] modification in an ES cell genome can be transmitted, by the breeding of ES cell/wild-type chimaeras, to generate mice containing the desired mutations in all cells.…”

Section: Embryonic Stem (Es) and Induced Pluripotent Stem (Ips) Cellsmentioning

confidence: 99%

“…In addition, it is possible to target reporter genes under the control of specific promoters to study gene expression patterns in different cell types. Furthermore, the ability of ES cells to differentiate in vitro to many different mature somatic cell types, in combination with purification of the cell of interest by methods such as directed differentiation and lineage selection, opens up the opportunity to use these mature cell types for various basic and therapeutical applications [3]. Unfortunately, not every mouse model is permissive for true ES cells derivation.…”

Section: Embryonic Stem (Es) and Induced Pluripotent Stem (Ips) Cellsmentioning

confidence: 99%

See 1 more Smart Citation

MicroRNAs and Induced Pluripotent Stem Cells for Human Disease Mouse Modeling

Underbayev¹,

Kasar²,

Yao³

et al. 2012

Journal of Biomedicine and Biotechnology

View full text Add to dashboard Cite

Human disease animal models are absolutely invaluable tools for our understanding of mechanisms involved in both physiological and pathological processes. By studying various genetic abnormalities in these organisms we can get a better insight into potential candidate genes responsible for human disease development. To this point a mouse represents one of the most used and convenient species for human disease modeling. Hundreds if not thousands of inbred, congenic, and transgenic mouse models have been created and are now extensively utilized in the research labs worldwide. Importantly, pluripotent stem cells play a significant role in developing new genetically engineered mice with the desired human disease-like phenotype. Induced pluripotent stem (iPS) cells which represent reprogramming of somatic cells into pluripotent stem cells represent a significant advancement in research armament. The novel application of microRNA manipulation both in the generation of iPS cells and subsequent lineage-directed differentiation is discussed. Potential applications of induced pluripotent stem cell—a relatively new type of pluripotent stem cells—for human disease modeling by employing human iPS cells derived from normal and diseased somatic cells and iPS cells derived from mouse models of human disease may lead to uncovering of disease mechanisms and novel therapies.

show abstract

ES cell technology: An introduction to genetic manipulation, differentiation and therapeutic cloning

Cited by 13 publications

References 101 publications

Primate Embryonic Stem Cells Proceed to Early GametogenesisIn Vitro

Primate Embryonic Stem Cells Proceed to Early GametogenesisIn Vitro

Kinetic and metabolic analysis of mouse embryonic stem cell expansion under serum-free conditions

MicroRNAs and Induced Pluripotent Stem Cells for Human Disease Mouse Modeling

Contact Info

Product

Resources

About