Erratum: Multiplexed ion beam imaging analysis for quantitation of protein expresssion in cancer tissue sections

Rost, Sandra; Bordeaux, Jennifer; Hitzman, Chuck; Koeppen, Hartmut; Liu, Scot D.

doi:10.1038/labinvest.2017.94

Cited by 8 publications

(8 citation statements)

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“…Recent attempts to address some aspects of these challenges have used a variety of methods 50 , 59 – 65 . Both mass cytometry 61 and ion beam imaging 65 can detect multiple markers. These methods analyze HER2 expression levels using mean pixel values extracted from image data.…”

Section: Discussionmentioning

confidence: 99%

Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2

Tobin

Wakefield

Jones

et al. 2018

Sci Rep

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All breast cancers are assessed for levels of human epidermal growth factor receptor 2 (HER2). Fluorescence in situ hybridization (FISH) and immunohistochemistry are currently used to determine if a patient is eligible for anti-HER2 therapy. Limitations of both tests include variability and relatively long processing times. Additionally, neither test determines whether HER2 contains the extracellular domain. While truncated in some tumors, this domain is required for binding of the therapeutic antibody trastuzumab. Here, trastuzumab was used to directly detect HER2 with quantitative single molecule localization microscopy (qSMLM). In proof of concept studies, our new method rapidly quantified both HER2 density and features of nano-organization. In cultured cells, the method was sensitive to subtle variations in HER2 expression. To assess patient samples, we combined qSMLM with tissue touch preparation (touch prep-qSMLM) and examined large areas of intact membranes. For cell lines and patient samples, HER2 copy numbers from FISH showed a significant positive correlation with detected densities from qSMLM and trended with HER2 cluster occupancy.

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Section: Discussionmentioning

confidence: 99%

Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2

Tobin

Wakefield

Jones

et al. 2018

Sci Rep

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“…With this technique, individual cells can be assessed with extraordinary fidelity according to the antibodies included in the panel, almost equal to the fidelity of assessment seen in the bulk population, such that even rare cell populations can be studied. This technology therefore has an important role in translational oncology research [11][12][13][14][15]. Thus, multiplexed IF can help characterize the topography of immune cells in cancer in detail, including the relative localizations and interactions of marker expression on cancer cells, immune cells, stromal cells, and endothelial cells, furthering our understanding of the disease [8,16].…”

Section: Introductionmentioning

confidence: 99%

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies

Parra

Jiang

Solis

et al. 2020

Cancers

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In the development of a multiplex immunofluorescence (IF) platform and the optimization and validation of new multiplex IF panels using a tyramide signal amplification system, several technical requirements are important for high-quality staining, analysis, and results. The aim of this review is to discuss the basic requirements for performing multiplex IF tyramide signal amplification (TSA) in formalin-fixed, paraffin-embedded cancer tissues to support translational oncology research. Our laboratory has stained approximately 4000 formalin-fixed, paraffin-embedded tumor samples using the multiplex IF TSA system for immune profiling of several labeled biomarkers in a single slide to elucidate cancer biology at a protein level and identify therapeutic targets and biomarkers. By analyzing several proteins in thousands of cells on a single slide, this technique provides a systems-level view of various processes in various tumor tissues. Although this technology shows high flexibility in cancer studies, it presents several challenges when applied to study different histology cancers. Our experience shows that adequate antibody validation, staining optimization, analysis strategies, and data generation are important steps for generating quality results. Tissue management, fixation procedures, storage, and cutting can also affect the results of the assay and must be standardized. Overall, this method is reliable for supporting translational research given a precise, step-by-step approach.

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“…Analyzes involved ligands, infiltration quality, co-stimulatory/inhibitory profile, and microenvironment. Several assays such as whole exome sequencing (WES), protein array, flow/mass cytometry (CyTOF), multicolor immunohistochemistry (IHC), Multiplexed Ion Beam Imaging (MIBI), Systematic evolution of ligands by exponential enrichment (SELEX), epigenetic modification, and B/T cell receptor repertoire sequencing have been used to pursue potential biomarkers and contribute for the future of cancer immunotherapy [93][94][95][96]. Also, these studies have the potential to elucidate immunological mechanisms of antitumor responses, monitor disease progression, evaluate the therapeutic effect, identify candidates for immunotherapy, and serve as prognostic markers of clinical outcome.…”

Section: Cancer Immune Monitoringmentioning

confidence: 99%

Immune Checkpoint Blockade and Immune Monitoring

Scutti¹,

Travassos²,

Vence³

2018

Immunoregulatory Aspects of Immunotherapy

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The concept of immunological surveillance, a monitoring process in which the immune system detects and destroys by several effector mechanisms, virally infected and neoplastic transformed cells in the body, was developed more than 50 years ago. Based on current research, it is clear that the immune system can recognize and eliminate transformed cells. An increasing number of studies has investigated the immune system in cancer patients and how it is prone to immunosuppression, due in part to the decrease of lymphocyte proliferation and cytotoxic activity. Such weakened immune system is then unable to fully accomplish its role in immunological surveillance, allowing nascent transformed cells to escape the selective pressure of the immune system. The main goal of cancer immunotherapy has been to reawaken the immune system from a suppressive slumber to enable it to attack cancer cells once again. As the results from the last 10 years attest, cancer immunotherapy is the best strategy to restore the activity of the immune system and unleash its potential to destroy cancer cells in cancer patients. This chapter aims to discuss the recent findings on immune monitoring studies and the use of immune checkpoint inhibition in cancer immunotherapy.

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Erratum: Multiplexed ion beam imaging analysis for quantitation of protein expresssion in cancer tissue sections

Abstract: In the title of this article, the word 'expression' is misspelled. The correct title is 'Multiplexed ion beam imaging analysis for quantitation of protein expression in cancer tissue sections.'

Cited by 8 publications

References 0 publications

Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2

Single molecule localization microscopy coupled with touch preparation for the quantification of trastuzumab-bound HER2

Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies

Immune Checkpoint Blockade and Immune Monitoring

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