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“…DR regimens are effective in slowing aging, and maintaining healthy status in animals [ 6 , 7 ]. Adipose mass quickly and dynamically responds to nutrient/energy fluctuation and its remodelling seems to mediate the beneficial effects of DR [ 7 ]. In this section we evaluated the effects of DR on adipose mass (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…DR regimens are effective in slowing aging, and maintaining healthy status in animals [ 6 , 7 ]. Adipose mass quickly and dynamically responds to nutrient/energy fluctuation and its remodelling seems to mediate the beneficial effects of DR [ 7 ]. In this section we evaluated the effects of DR on adipose mass (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…We previously demonstrated that lowering calorie intake through one-day fasting enhanced the expression of mitochondrial genes in sAT [ 17 ]. To more strictly establish whether the expression of mitochondrial genes varies in response to calorie intake and age in sAT, we moved at searching publically available gene expression data sets.…”
Section: Resultsmentioning
confidence: 99%
“…Cytoplasmic fraction was obtained by using a commercially available kit (NE-PER ® extraction kit, according to manufacturer's instructions (Thermo Scientific, Milwaukee, WI, USA). Crude mitochondrial fractions were obtained as previously described [ 17 ].…”
Section: Methodsmentioning
confidence: 99%