Erratum: Antimycobacterial Activity of Alkaloids and Extracts from Tabernaemontana alba and T. arborea

Potent Anti-amoebic Effects of Ibogaine, Voacangine and the Root Bark Alkaloid Fraction of Tabernaemontana arborea

“…IC50 median values obtained were very close to the metronidazole IC50 of 1.17 µg/mL (6.8 μM) previously reported by us [30]. Considering the CC50 values on VERO cells previously reported by our group [21], the SI obtained for the alkaloid fraction of T. arborea root bark and the isolated alkaloids Iibogaine and voacangine were of 10.14, 252.8 and 13.6, respectively (Table 1).…”

“…In a previous report, our group identified MIAs ibogaine and voacangine in T. arborea root bark methanol extract 21 . In contrast to the moderate antimycobacterial activity shown by these alkaloids (IC 50 between 16 and 40 µg/mL), both showed potent amoebicidal activity, causing the death of all parasites in 24 h at a concentration of 10 µg/mL and yielding an IC 50 between 1.4 and 3.5 µg/mL, as mentioned above.…”

“…Crude extracts from the root bark were obtained by extraction with methanol, from which the alkaloid fraction was obtained by acid-base extraction and analyzed by GC-MS, and HPLC as described in 21 , and where the chromatograms can be consulted. Pure ibogaine and voacangine kindly donated by Phytostan Enterprises, Inc. were used as external standards in these analyses.…”

“…For instance, T. alba Mill., a species found from the South of México to Panama, is used in traditional medicine to treat skin conditions due to their anti-inflammatory, analgesic, and anti-microbial properties [15]. In addition to the well-known antibacterial effect of extracts and alkaloid fractions of several Tabernaemontana species plants [16][17][18][19][20][21], anti-parasitic effects…”

“…For instance, T. alba Mill., a species found from the south of México to Panama, is used in traditional medicine to treat skin conditions due to their anti-inflammatory, analgesic, and anti-microbial properties 15 . In addition to the well-known antibacterial effect of extracts and alkaloid fractions of several Tabernaemontana species plants 16 , 17 , 18 , 19 , 20 , 21 , anti-parasitic effects have also been reported, including extracts and alkaloids from T. citrifolia on the gastrointestinal nematode Haemonchus contortus 22 , T. elegans, T. pachysiphon, T. peduncularis and T. macrocarpa on the malarial protozoan Plasmodium falciparum 23 , 24 , 25 , 26 , T. pandacaqui on the protozoan Trypanosoma cruzi , causal agent of Chagas disease 27 and T. longipes on Trypanosoma brucei , the causal agent of African sleeping sickness 28 . Previous phytochemical research has shown T. arborea root bark to be particularly rich in monoterpenoid indole alkaloids (MIAs), including ibogaine, voacangine, and vobasine, as well as the bis-indole type alkaloid voacamine 21 , 29 .…”