Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70

Bedirli, Abdülkadir; Şakrak, Ömer; Muhtaroğlu, Sebahattin; Soyuer, Işın; Güler, İlkay; Erdoğan, Ali; Sözüer, Erdoğan

doi:10.1016/j.jss.2004.06.016

Cited by 55 publications

(45 citation statements)

References 22 publications

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“…In certain experiments, chronic treatment with ergothioneine (10-1000 mg/kg per day) was started on day 0. This dosage range of ergothioneine has been used in other studies and no toxicity was observed (Moncaster et al, 2002;Bedirli et al, 2004). In the rat, a steady blood concentration of 20 mg/ml (∼87 mM) is achieved when a single intravenous dose of 100 mg/kg ergothioneine is given (Kaneko et al, 1980).…”

Section: Methodsmentioning

confidence: 92%

“…liver by increasing heat shock proteins-70 and decreasing tumor necrosis factor-a, interleukin-1b, malondialdehyde, and myeloperoxidase levels in the tissues (Bedirli et al, 2004;Sakrak et al, 2008). Ergothioneine is obtained from diet and its concentration in human blood ranges from 11.9 to 13.5 mg/l (i.e., 51.9 to 58.9 mM) for normal subjects, 11.6 to 13.6 mg/l (i.e., 50.6 to 60.1 mM) for type I diabetic subjects, and 11.9 to 21.9 (i.e., 51.9 to 95.5 mM) for type II diabetic subjects (Epand et al, 1988).…”

Section: Ergothioneine Protects Endothelial Cellsmentioning

confidence: 99%

“…Oral administration of ergothioneine protects fatty acids against ferric-nitrilotriacetate-induced oxidation in rat kidney and liver (Deiana et al, 2004). In vivo treatment of rats with ergothioneine also protects intestinal and liver tissues from damage during ischemia/reperfusion injury (Bedirli et al, 2004;Sakrak et al, 2008). In addition, chronic administration of ergothioneine inhibits brain lipid peroxidation, protects against cisplatin-induced neuronal injury, and enhances cognition in the mouse (Song et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

Yang²,

Sit

et al. 2014

J Pharmacol Exp Ther

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Ergothioneine is a thiourea derivative of histidine found in food, especially mushrooms. Experiments in cell-free systems and chemical assays identified this compound as a powerful antioxidant. Experiments were designed to test the ability of endothelial cells to take up ergothioneine and hence benefit from protection against oxidative stress. Reverse-transcription polymerase chain reaction and Western blotting demonstrated transcription and translation of an ergothioneine transporter in human brain microvascular endothelial cells (HBMECs). Uptake of [3 H]ergothioneine occurred by the organic cation transporter novel type-1 (OCTN-1), was sodium-dependent, and was reduced when expression of OCTN-1 was silenced by small interfering RNA (siRNA). The effect of ergothioneine on the production of reactive oxygen species (ROS) in HBMECs was measured using dichlorodihydrofluorescein and lucigenin, and the effect on cell viability was studied using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. ROS production and cell death induced by pyrogallol, xanthine oxidase plus xanthine, and high glucose were suppressed by ergothioneine. The antioxidant and cytoprotective effects of ergothioneine were abolished when OCTN-1 was silenced using siRNA. The expression of NADPH oxidase 1 was decreased, and those of glutathione reductase, catalase, and superoxide dismutase enhanced by the compound. In isolated rat basilar arteries, ergothioneine attenuated the reduction in acetylcholine-induced relaxation caused by pyrogallol, xanthine oxidase plus xanthine, or incubation in high glucose. Chronic treatment with the compound improved the response to acetylcholine in arteries of rats with streptozotocin-induced diabetes. In summary, ergothioneine is taken up by endothelial cells via OCTN-1, where the compound then protects against oxidative stress, curtailing endothelial dysfunction.

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Section: Methodsmentioning

confidence: 92%

Section: Ergothioneine Protects Endothelial Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

Yang²,

Sit

et al. 2014

J Pharmacol Exp Ther

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“…Dysfunction of the liver sinusoidal cells has been observed during arsenic-induced oxidative stress, leading to vascular disease atherosclerosis (States et al, 2009). Bedirli et al (2004) have demonstrated that exogenous administration of ERGO improves survival in animals with liver injury induced by ischemia and reperfusion. Thus, ERGO may function as one of the defense mechanisms acting under such pathological conditions.…”

Section: Discussionmentioning

confidence: 99%

Functional Expression of Carnitine/Organic Cation Transporter OCTN1/SLC22A4 in Mouse Small Intestine and Liver

et al. 2010

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ABSTRACT:Carnitine/organic cation transporter (OCTN1/SLC22A4) accepts various therapeutic agents as substrates in vitro and is expressed ubiquitously, although its function in most organs has not yet been examined. The purpose of the present study was to evaluate functional expression of OCTN1 in small intestine and liver, using octn1 gene knockout [octn1(؊/؊)] mice. After oral administration of [

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“…The HO system is among the most critical of the cytoprotective mechanisms activated during cellular stress, hyperthermic preconditioning, or long-term fasting, exerting antioxidant and anti-inflammatory functions, acting as a molecular chaperon, modulating cell cycle, and maintaining microcirculation (3,9,20).…”

mentioning

confidence: 99%

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

Acquaviva¹,

Lanteri²,

G³

et al. 2009

American Journal of Physiology-Gastrointestinal and Liver Physi

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following liver ischemia results in oxidative stress leading to liver injury. The aim of this study was to investigate the combined effects of two antioxidant agents, rutin and L-arginine, in rat liver ischemia-reperfusion (I/R). Male Wistar rats were divided into five groups: 1) sham operated, 2) I/R, 3) I/R ϩ rutin, 4) I/R ϩ L-arginine, and 5) I/R ϩ rutin ϩ L-arginine. Plasmatic and hepatic levels of alanine transaminase (ALT), aspartate transaminase (AST), lipid peroxides (LOOH), and thiol groups (RSH) were examined, as well as DNA fragmentation and liver histopathology. Furthermore, to elucidate the pathophysiological processes involved in the antioxidant mechanism(s) of rutin and L-arginine, we assessed the expression of inducible (iNOS) and endothelial nitric oxide synthase (eNOS) isoforms and heme oxygenase-1 (HO-1), both playing key roles in the biochemical cascade of liver injury. Significant increase in plasmatic ALT and AST activities were observed in untreated I/R rats compared with sham-operated animals, whereas treatment with rutin or L-arginine in I/R rats reduced hepatic damage. Interestingly, combined therapy with rutin and Larginine resulted in a further reduction of plasmatic ALT and AST activities compared with rutin or L-arginine alone. These results were further confirmed by the analysis of DNA fragmentation, LOOH,

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Ergothioneine pretreatment protects the liver from ischemia-reperfusion injury caused by increasing hepatic heat shock protein 70

Cited by 55 publications

References 22 publications

Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

Uptake and Protective Effects of Ergothioneine in Human Endothelial Cells

Functional Expression of Carnitine/Organic Cation Transporter OCTN1/SLC22A4 in Mouse Small Intestine and Liver

Beneficial effects of rutin andl-arginine coadministration in a rat model of liver ischemia-reperfusion injury

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