Ergot Alkaloid Glycosides from Saprophytic Cultures of Claviceps, II. Chanoclavine I Fructosides

Flieger, Miroslav; Křen, Vladimı́r; Zelenkova, N. F.; Sedmera, Petr; Novák, J. J. K.; Sajdl, P.

doi:10.1021/np50067a025

Cited by 23 publications

(9 citation statements)

References 3 publications

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“…2B showed positive ESI-MS, m/z 257 (M+H). The 1 H and 13 C NMR data of the alkaloid (data not shown) matched with the reported NMR data for chanoclavine in literature [27].…”

Section: Chanoclavinesupporting

confidence: 85%

Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Maurya

Srivastava

2009

Journal of Chromatography B

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“…2B showed positive ESI-MS, m/z 257 (M+H). The 1 H and 13 C NMR data of the alkaloid (data not shown) matched with the reported NMR data for chanoclavine in literature [27].…”

Section: Chanoclavinesupporting

confidence: 85%

Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Maurya

Srivastava

2009

Journal of Chromatography B

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“…In most of the published work on HPLC of ergot alkaloids, the aprotic solvent acetonitrile has been used as the nonpolar solvent in the mobile phase; exceptions come from the work of Flieger and colleagues, who have used the protic solvent methanol in place of acetonitrile (e.g., Flieger et al, 1990;Shelby, Olsovska, Havlicek, & Flieger, 1997). In most of the published work on HPLC of ergot alkaloids, the aprotic solvent acetonitrile has been used as the nonpolar solvent in the mobile phase; exceptions come from the work of Flieger and colleagues, who have used the protic solvent methanol in place of acetonitrile (e.g., Flieger et al, 1990;Shelby, Olsovska, Havlicek, & Flieger, 1997).…”

Section: Alternative Methodsmentioning

confidence: 99%

“…In most of the published work on HPLC of ergot alkaloids, the aprotic solvent acetonitrile has been used as the nonpolar solvent in the mobile phase; exceptions come from the work of Flieger and colleagues, who have used the protic solvent methanol in place of acetonitrile (e.g., Flieger et al, 1990;Shelby, Olsovska, Havlicek, & Flieger, 1997). Various protocols call for ammonium acetate (Spiering et al, 2002), ammonium carbonate (Rottinghaus et al, 1991), or ammonium hydroxide (Flieger et al, 1990;. To measure the much more polar ergot alkaloid lysergic acid and its 8-epimer, Lodge-Ivy, Walker, Fleischmann, True, and Craig (2006) used an isocratic system with much lower acetonitrile (6%).…”

Section: Alternative Methodsmentioning

confidence: 99%

Analysis and Modification of Ergot Alkaloid Profiles in Fungi

Panaccione¹,

Ryan²,

Schardl³

et al. 2012

Methods in Enzymology

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“…The reference marker compounds, lysergol (1) and chanoclavine (2), were isolated in high purity by fast centrifugal partition chromatography (FCPC; Maurya and Srivastava, 2009) and characterized on the basis of their spectroscopic data (Kren et al, 1997;Flieger et al, 1990).…”

Section: Isolation and Characterization Of Reference Compoundsmentioning

confidence: 99%

Quantitative determination of bioactive alkaloids lysergol and chanoclavine in Ipomoea muricata by reversed‐phase high‐performance liquid chromatography

Maurya

Verma

Srivastava

2011

Biomedical Chromatography

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A rapid, simple, sensitive, gradient and reproducible, reverse-phase high-performance liquid chromatographic method was developed for the quantitative estimation of bioactive alkaloids, lysergol and chanoclavine in the seeds of Ipomoea muricata. The clavine alkaloid, lysergol, is a bioenhancer for the drugs and nutrients. The samples were analyzed by reverse-phase chromatography on a Waters spherisorb ODS2 column (250 × 4.6 mm, i.d., 10 µm) using binary gradient elution with acetonitrile and 0.01 m phosphate buffer (NaH₂PO₄) containing 0.1% glacial acetic acid at a flow rate of 0.8 mL/min, a column temperature of 25 °C and UV detection at λ 254 nm. The limits of detection (LOD) and quantitation (LOQ) were 0.035 and 0.106 µg/mL for lysergol and 0.039 and 0.118 µg/mL for chanoclavine, respectively. Standard curves were linear in the range of 2-10 µg/mL (r > 99) for both analytes. Good results were achieved with respect to repeatability (RSD < 2%) and recovery (99.20-102.0). The method was validated for linearity, accuracy repeatability, LOQ and LOD. The method is simple, accurate and precise, and may be recommended for routine quality control analysis of I. muricata seed extracts containing these two clavine alkaloids (1, 2) as bioactive principles of the herb.

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Ergot Alkaloid Glycosides from Saprophytic Cultures of Claviceps, II. Chanoclavine I Fructosides

Cited by 23 publications

References 3 publications

Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Large-scale separation of clavine alkaloids from Ipomoea muricata by pH-zone-refining centrifugal partition chromatography

Analysis and Modification of Ergot Alkaloid Profiles in Fungi

Quantitative determination of bioactive alkaloids lysergol and chanoclavine in Ipomoea muricata by reversed‐phase high‐performance liquid chromatography

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