Equine Sperm Selection by Synthetic Membrane Filter

Larentis, Gustavo Rupp; Camozzato, Giovani Casanova; Bastos, H.B.A.; Gregory, Ricardo Macedo; Mattos, Rodrigo Costa

doi:10.1016/j.jevs.2018.01.013

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…4 ). In contrast to previous reports where diffusion occurs horizontally 19 , the current design takes advantage of the gravity as the diffusion occurs vertically. This design was conceptualized so that diffusion would occur faster and prevent backflow of sperm cells which had already crossed the membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Commercially available fibrous poly(ester) membrane devices (Pall Biosupport Corporation) were attached to a pipette tip, allowing for the isolation of motile sperm cells from semen samples and the separation of other types of cells and morphologically abnormal sperm cells 16 . Poly(carbonate) track etched (PTCE) membranes with cylindrical pores have also been successfully used to isolate motile spermatozoa from semen sample 19 . These reports in isolating motile spermatozoa propose different techniques with different configurations but do not address the role of membranes in the separation process, particularly exploring different filter characteristic for optimal isolation.…”

Section: Introductionmentioning

confidence: 99%

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Isolating motile sperm cell sorting using biocompatible electrospun membranes

Katigbak

Dumée

Kong

2022

Sci Rep

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Motility is an indicator of sperm cell viability due to higher probability in swimming through the female reproductive tract and undergo fertilization with the egg cell. Centrifugation method is a technique to process high volume semen and isolate motile sperm cells but decreases the biochemical integrity of spermatozoa due to the contact with reactive oxygen species (ROS) from dead cells released during centrifugation. This study uses solution electrospun poly(ε-caprolactone) membranes as an alternative in isolating motile spermatozoa by utilizing a rationally designed 3D printed module set up, providing the same benefits as commercially available techniques with minimal processing time, and bypassing the centrifugation step to provide higher quality sperm cells. The membranes, with nominal pore size distributions ranging from 5 to 6 µm are highly porous structures suitable for establishing baseline data for sperm cell sorting by motility. The proposed method allows for isolation of motile sperm cells with 74% purity, while decreasing the processing time by 98% when compared to centrifugation techniques. This novel approach provides a facile method for isolating motile spermatozoa directly from frozen semen samples without any pretreatments and is easily scalable for small and medium scale farms as well as larger industries.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Isolating motile sperm cell sorting using biocompatible electrospun membranes

Katigbak

Dumée

Kong

2022

Sci Rep

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“…Biological membranes include cell membranes (outer coverings of cells or organelles that allow passage of certain constituents) [ 37 , 38 ]; nuclear membranes, which cover a cell nucleus [ 39 ]; and tissue membranes [ 40 , 41 ], such as mucosae and serosae. Synthetic membranes are made by humans for use in laboratories and industry (such as chemical plants) [ 42 ]. Polysulfone, polyether sulfone, polyvinylidene fluoride (hydrophobic polyvinylidene fluoride, hydrophilic polyvinylidene fluoride), polytetrafluoroethylene, polypropylene, modified acrylics, and others make up the global medical membrane market.…”

Section: Introductionmentioning

confidence: 99%

Membrane Technological Pathways and Inherent Structure of Bacterial Cellulose Composites for Drug Delivery

et al. 2021

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This report summarizes efforts undertaken in the area of drug delivery, with a look at further efforts made in the area of bacterial cellulose (BC) biomedical applications in general. There are many current methodologies (past and present) for the creation of BC membrane composites custom-engineered with drug delivery functionality, with brief consideration for very close applications within the broader category of biomedicine. The most emphasis was placed on the crucial aspects that open the door to the possibility of drug delivery or the potential for use as drug carriers. Additionally, consideration has been given to laboratory explorations as well as already established BC-drug delivery systems (DDS) that are either on the market commercially or have been patented in anticipation of future commercialization. The cellulose producing strains, current synthesis and growth pathways, critical aspects and intrinsic morphological features of BC were given maximum consideration, among other crucial aspects of BC DDS.

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