Epithelial to Mesenchymal Transition and Progression of Glioblastoma

Paľa, Andrej; Karpel‐Massler, Georg; Wirtz, Christian Rainer; Halatsch, Marc Eric

doi:10.5772/53183

Cited by 5 publications

(2 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The composition of perivascular niche (PVN) has been carefully described, with also the inclusion of extra-cellular matrix, integrins, cell adhesion signaling, cadherin family, and so forth [ 20 ]. In GBMs, strong evidence for the existence of the epithelial-to-mesenchymal transition (EMT) process is still lacking, but this process is increasingly reported as instrumental to growth and diffusion of the tumor [ 21 , 22 ]. EMT is a process letting differentiated epithelial cells establish stable contacts with neighbor cells, assume a mesenchymal cell phenotype with loss of cell-cell interactions, reduced cellular adhesion, active production of ECM proteases, increased cytoskeletal dynamics, and changes in transcription factor expression, and assume the acquisition of a stem cell program; all of them lead to increased migration and invasion ability.…”

Section: Discussionmentioning

confidence: 99%

Stem Cell Niches in Glioblastoma: A Neuropathological View

Schiffer

Mellai

Annovazzi

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

Glioblastoma (GBM) stem cells (GSCs), responsible for tumor growth, recurrence, and resistance to therapies, are considered the real therapeutic target, if they had no molecular mechanisms of resistance, in comparison with the mass of more differentiated cells which are insensitive to therapies just because of being differentiated and nonproliferating. GSCs occur in tumor niches where both stemness status and angiogenesis are conditioned by the microenvironment. In both perivascular and perinecrotic niches, hypoxia plays a fundamental role. Fifteen glioblastomas have been studied by immunohistochemistry and immunofluorescence for stemness and differentiation antigens. It has been found that circumscribed necroses develop inside hyperproliferating areas that are characterized by high expression of stemness antigens. Necrosis developed inside them because of the imbalance between the proliferation of tumor cells and endothelial cells; it reduces the number of GSCs to a thin ring around the former hyperproliferating area. The perinecrotic GSCs are nothing else that the survivors remnants of those populating hyperproliferating areas. In the tumor, GSCs coincide with malignant areas so that the need to detect where they are located is not so urgent.

show abstract

Section: Discussionmentioning

confidence: 99%

Stem Cell Niches in Glioblastoma: A Neuropathological View

Schiffer

Mellai

Annovazzi

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…One of these mechanisms includes epithelial-mesenchymal transition (EMT), a complex process allowing cells with epithelial characteristics to gain mesenchymal properties due to highly regulated changes in gene expression (7). While epithelial-like cells show a polarized subtype and are likely to engage in intracellular adhesion, mesenchymal differentiated cells present with altered polarization, higher migratory capacity and stem cell properties (8)(9)(10). This transition is valuable in processes such as embryogenesis and wound healing, but it leads to increased tumor progression, chemoresistance and mitotic progression (11).…”

Section: Introductionmentioning

confidence: 99%

ZEB1 induces N‑cadherin expression in human glioblastoma and may alter patient survival

et al. 2022

View full text Add to dashboard Cite

The present study investigated the expression of epithelial-mesenchymal transition (EMT)-related factors zinc finger E-box-binding homeobox 1 (ZEB1), cadherin-1 (CDH1), cadherin-2 (CDH2) and the cell cycle modulating kinase cyclin-dependent kinase 1 (CDK1) in human glioblastoma (GBM) compared to normal brain tissue, as well as whether the levels of expression were associated with the overall and progression-free survival of the GBM patients. In 44 GBM and five normal brain tissue specimens, the expression levels of ZEB1, CDH1, CDH2 and CDK1 were evaluated by real-time PCR and immunostaining, and the results were correlated with clinical data. The expression levels of all investigated genes as detected by immunostaining were significantly higher in the GBM when compared to the normal brain tissues. There was no influence on survival. A linear correlation between ZEB1 and CDH2 and CDK1 expression was observed in GBM. Moreover, ZEB1 was involved in EMT (e.g., signaling in human GBM) and high ZEB1 levels were linked to an aberrant cell cycle processing, marked by CDK1 overexpression.

show abstract