Epinephrine regulates cholinergic transmission mediated by rat retinal neurons in culture

Tsai, Wen-Chi; Koh, Seishu; Puro, Donald G.

doi:10.1016/0306-4522(87)90363-0

Cited by 7 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In normoxic retinas, AC basal activity was similar in WT and sst1 KO, but it was significantly reduced in sst2 KO retinas (-21%). The enzyme was potently stimulated by FSK and it was also activated by epinephrine, whose receptors, positively coupled to AC, are expressed in the rodent retina [33]. FSK-stimulated AC activity was insensitive to SRIF or OCTR in WT and in sst2 KO retinas while, as expected [12], SRIF or OCTR greatly reduced FSK-stimulated AC activity (-47% and -51%, respectively) in sst1 KO retinas.…”

Section: Resultsmentioning

confidence: 91%

Adenylyl Cyclase/cAMP System Involvement in the Antiangiogenic Effect of Somatostatin in the Retina. Results from Transgenic Mice

et al. 2008

View full text Add to dashboard Cite

Neoangiogenesis is a response to retinal hypoxia that is inhibited by somatostatin (SRIF) through its subtype 2 receptor (sst2). Using a mouse model of hypoxia-induced retinopathy, we investigated whether inhibition of adenylyl cyclase (AC) is involved in SRIF anti-angiogenic actions. Hypoxia increased AC responsiveness in wild type (WT) retinas and in retinas lacking sst2, but not in sst2-overexpressing retinas. Hypoxia also altered AC isoform expression with different patterns depending on sst2 expression level. The AC VII isoform mRNA and protein resulted the most affected. Indeed, in hypoxia AC VII expression was enhanced in WT retinas and it was further increased in sst2-lacking retinas, whereas in sst2 overexpressing retinas the increase of AC VII was lower than in WT retinas. These data suggest an involvement of AC/cAMP in mediating both hypoxia-evoked retinal neoangiogenesis and SRIF protective actions. The AC VII isoform is a candidate to a main role in these mechanisms.

show abstract

Section: Resultsmentioning

confidence: 91%

Adenylyl Cyclase/cAMP System Involvement in the Antiangiogenic Effect of Somatostatin in the Retina. Results from Transgenic Mice

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Apart from 5-HT, there are also some reports suggesting that both NE and E are the neurotransmitters in the retina[35–37] and previous evidence proved that NE and E represented approximately 5% of the DA concentration[38]. But few researches have been performed to investigate the alterations in the levels of NE and E in myopia development.…”

Section: Discussionmentioning

confidence: 99%

Effects of Constant Flickering Light on Refractive Status, 5-HT and 5-HT2A Receptor in Guinea Pigs

Luo

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

PurposeTo investigate the effects of constant flickering light on refractive development, the role of serotonin (i.e.5-hydroxytryptamine, 5-HT)and 5-HT2A receptor in myopia induced by flickering light in guinea pigs.MethodsForty-five guinea pigs were randomly divided into three groups: control, form deprivation myopia (FDM) and flickering light induced myopia (FLM) groups(n = 15 for each group). The right eyes of the FDM group were covered with semitransparent hemispherical plastic shells serving as eye diffusers. Guinea pigs in FLM group were raised with illumination of a duty cycle of 50% at a flash frequency of 0.5Hz. The refractive status, axial length (AL), corneal radius of curvature(CRC) were measured by streak retinoscope, A-scan ultrasonography and keratometer, respectively. Ultramicroscopy images were taken by electron microscopy. The concentrations of 5-HTin the retina, vitreous body and retinal pigment epithelium (RPE) were assessed by high performance liquid chromatography, the retinal 5-HT2A receptor expression was evaluated by immunohistofluorescence and western blot.ResultsThe refraction of FDM and FLM eyes became myopic from some time point (the 4th week and the 6th week, respectively) in the course of the experiment, which was indicated by significantly decreased refraction and longer AL when compared with the controls (p<0.05). The concentrations of 5-HT in the retina, vitreous body and RPE of FDM and FLM eyes were significantly increased in comparison with those of control eyes (both p<0.05). Similar to FDM eyes, the expression of retinal 5-HT2A receptor in FLM eyes was significantly up-regulated compared to that of control eyes (both p<0.05). Western blot analysis showed that retinal 5-HT2A receptor level elevated less in the FLM eyes than that in the FDM eyes. Moreover, the levels of norepinephrine and epinephrine in FDM and FLM groups generally decreased when compared with control groups (all p<0.05).ConclusionsConstant flickering light could cause progressive myopia in guinea pigs. 5-HT and 5-HT2A receptor increased both in form deprivation myopia and flickering light induced myopia, indicating that 5-HT possibly involved in myopic development via binding to5-HT2A receptor.

show abstract

“…(http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0042841) reported that α‐7‐nicotinic acetylcholine receptors are present in the microvilli of RPE cells that envelope the tips of POSs. Epinephrines are mediated by cholinergic neurons and modulate neurotransmitters in the rat retina . Photoreceptors in most vertebrates including human, contain D2 DA receptors and consequently retinal DA are necessary for the circadian nature of light‐adapted vision as well as optimal contrast detection and acuity …”

Section: Discussionmentioning

confidence: 99%

Analysis of fine structure and biochemical changes of retina during aging of Wistar albino rats

El‐Sayyad

Khalifa

El-Sayyad

et al. 2013

Clinical Exper Ophthalmology

View full text Add to dashboard Cite

show abstract

Epinephrine regulates cholinergic transmission mediated by rat retinal neurons in culture

Cited by 7 publications

References 20 publications

Adenylyl Cyclase/cAMP System Involvement in the Antiangiogenic Effect of Somatostatin in the Retina. Results from Transgenic Mice

Adenylyl Cyclase/cAMP System Involvement in the Antiangiogenic Effect of Somatostatin in the Retina. Results from Transgenic Mice

Effects of Constant Flickering Light on Refractive Status, 5-HT and 5-HT2A Receptor in Guinea Pigs

Analysis of fine structure and biochemical changes of retina during aging of Wistar albino rats

Contact Info

Product

Resources

About