2016
DOI: 10.18632/aging.100972
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Abstract: Aging is reflected by highly reproducible DNA methylation (DNAm) changes that open new perspectives for estimation of chronological age in legal medicine. DNA can be harvested non-invasively from cells at the inside of a person's cheek using buccal swabs – but these specimens resemble heterogeneous mixtures of buccal epithelial cells and leukocytes with different epigenetic makeup. In this study, we have trained an age predictor based on three age-associated CpG sites (associated with the genes PDE4C, ASPA, an… Show more

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Cited by 76 publications
(86 citation statements)
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References 40 publications
(64 reference statements)
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“…The accuracy could be slightly increased by Lasso ( Figure 3E) and elastic net algorithms ( Figure 3F) that were generated based on all CpGs of the amplicons, but it remained lower than 12 for blood samples. This might be due to the heterogeneous composition of buccal epithelial cells and leukocytes in buccal swab samples (Eipel et al 2016).…”
Section: Epigenetic Age-predictions With Bisulfite Barcoded Ampliconmentioning
confidence: 99%
“…The accuracy could be slightly increased by Lasso ( Figure 3E) and elastic net algorithms ( Figure 3F) that were generated based on all CpGs of the amplicons, but it remained lower than 12 for blood samples. This might be due to the heterogeneous composition of buccal epithelial cells and leukocytes in buccal swab samples (Eipel et al 2016).…”
Section: Epigenetic Age-predictions With Bisulfite Barcoded Ampliconmentioning
confidence: 99%
“…The separation by age still held when cohort effects were considered ( Figure S4-6). These data demonstrate that development and age modify the buccal swab methylome [19,23,25,26]. PC2, which accounts for 20.7% of the variance, was the dominant component distinguishing samples of different ages ( Figure 2B).…”
Section: Methylation Level Changes Across Individuals Reflect Postnatmentioning
confidence: 70%
“…Third, because buccal swabs can contain a mixture of different cells (mainly buccal epithelial cells and leukocytes) we tested the potential influence of cell‐type heterogeneity on our findings, using two independent methods. The first derives a “predicted tissue” based on DNAm patterns (Horvath, ), whereas the second uses two CpG sites to estimate the percentage of buccal epithelial cells for each individual (Eipel et al, ). Each of these estimates was then examined in relation to DRD4 methylation and physical aggression (see the Supporting Information Methods for full details and results).…”
Section: Resultsmentioning
confidence: 99%