Epidermal growth factor receptor (EGFR) signaling in cancer

Normanno, Nicola; Luca, Antonella De; Bianco, Caterina; Strizzi, Luigi; Mancino, Mario; Maiello, Monica R.; Carotenuto, Adele; Feo, Gianfranco De; Caponigro, Francesco; Salomon, David

doi:10.1016/j.gene.2005.10.018

Cited by 1,665 publications

(1,325 citation statements)

References 157 publications

(174 reference statements)

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“…Additionally, changing density of reacting carbohydrate ligands may be accompanied with altering size and orientation of carbohydrates and the dimensional relationship between ligands and CBPs,25, 29 which will affect the accessibility of the carbohydrate ligands and finally promote or suppress the related processes. Moreover, alterations in protein glycosylations have been reported to perturb the structure and function of glycoproteins by changing their oligomerization, turnover, conformation, and interactions with other molecules 30, 31, 32, 33. Some computational models have also predicted that bulky glycoproteins favor transmembrane receptor organization (e.g., carbohydrate‐mediated integrin clustering would facilitate the assembly of mature adhesion complexes and enhance growth factor signaling) 34, 35.…”

Section: Resultsmentioning

confidence: 99%

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

et al. 2016

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Carbohydrate alterations on cell membranes are associated with various cancer processes, including tumorigenesis, malignant transformation, and tumor dissemination. However, variations in the distributions of cancer‐associated carbohydrates are unclear at the molecular level. Herein, direct stochastic optical reconstruction microscopy is used to reveal that seven major types of carbohydrates tended to form obvious clusters on cancer cell membranes compared with normal cell membranes (both cultured and primary cells), and most types of carbohydrates present a similar distributed characteristic on various cancer cells (e.g., HeLa and Os‐Rc‐2 cells). Significantly, sialic acid is found to distribute in larger‐sized clusters with a higher cluster coverage percentage on various cancer cells than normal cells. These findings on the aberrant distributions of cancer‐associated carbohydrates can potentially serve as novel diagnostic and therapeutic targets, as well as making a contribution to clarify how abnormal glycosylations of membrane glycoconjugates participate in tumorigenesis and metastasis.

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Section: Resultsmentioning

confidence: 99%

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

et al. 2016

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“…EGF stimulates the replication of granulosa cells, thereby inducing follicle growth [33]. The expression levels of EGFR have been correlated with the pathogenesis of a broad range of human cancers [34]. EGFR overexpression has been found to be a marker of poor prognosis [35,36].…”

Section: Discussionmentioning

confidence: 99%

Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells

Huang

Cheng

Nishi³

et al. 2010

Reprod Biol Endocrinol

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BackgroundHomeobox (HOX) genes encode transcription factors, which regulate cell proliferation, differentiation, adhesion, and migration. The deregulation of HOX genes is frequently associated with human reproductive system disorders. However, knowledge regarding the role of HOX genes in human granulosa cells is limited.MethodsTo determine the role of HOXA7 in the regulation and associated mechanisms of cell proliferation in human granulosa cells, HOXA7 and epidermal growth factor receptor (EGFR) expressions were examined in primary granulosa cells (hGCs), an immortalized human granulosa cell line, SVOG, and a granulosa tumor cell line, KGN, by real-time PCR and Western blotting. To manipulate the expression of HOXA7, the HOXA7 specific siRNA was used to knockdown HOXA7 in KGN. Conversely, HOXA7 was overexpressed in SVOG by transfection with the pcDNA3.1-HOAX7 vector. Cell proliferation was measured by the MTT assay.ResultsOur results show that HOXA7 and EGFR were overexpressed in KGN cells compared to hGCs and SVOG cells. Knockdown of HOXA7 in KGN cells significantly decreased cell proliferation and EGFR expression. Overexpression of HOXA7 in SVOG cells significantly promoted cell growth and EGFR expression. Moreover, the EGF-induced KGN proliferation was abrogated, and the activation of downstream signaling was diminished when HOXA7 was knocked down. Overexpression of HOXA7 in SVOG cells had an opposite effect.ConclusionsOur present study reveals a novel mechanistic role for HOXA7 in modulating granulosa cell proliferation via the regulation of EGFR. This finding contributes to the knowledge of the pro-proliferation effect of HOXA7 in granulosa cell growth and differentiation.

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“…Overexpression and mutation of ErbB family members and their ligands have been identified in cancers of the breast, lung, colon, stomach, pancreatic, ovary, brain, prostate, and kidney [153]. A massive effort has led to understanding of the mechanisms and mutations leading to unregulated ErbB signaling in human malignancies.…”

Section: Erbb Members In Diseasementioning

confidence: 99%

The epidermal growth factor receptor family: Biology driving targeted therapeutics

Wieduwilt

Moasser

2008

Cell. Mol. Life Sci.

596

446

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The epidermal growth factor family of receptor tyrosine kinases (ErbBs) plays essential roles in regulating cell proliferation, survival, differentiation and migration. The ErbB receptors carry out both redundant and restricted functions in mammalian development and in the maintenance of tissues in the adult mammal. Loss of regulation of the ErbB receptors underlies many human diseases, most notably cancer. Our understanding of the function and complex regulation of these receptors has fueled the development of targeted therapeutic agents for human malignancies in the last 15 years. Here we review the biology of ErbB receptors, including their structure, signaling, regulation, and roles in development and disease, then briefly touch on their increasing roles as targets for cancer therapy.

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Epidermal growth factor receptor (EGFR) signaling in cancer

Cited by 1,665 publications

References 157 publications

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

Variation in Carbohydrates between Cancer and Normal Cell Membranes Revealed by Super‐Resolution Fluorescence Imaging

Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells

The epidermal growth factor receptor family: Biology driving targeted therapeutics

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