Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling

Zhang, Haihua; Nan, Weixiao; Wang, Shiyong; Zhang, Tietao; Si, Huazhe; Yang, Fenghuan; Li, Guangyu

doi:10.1159/000445630

Cited by 47 publications

(49 citation statements)

References 28 publications

(27 reference statements)

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“…As shown in Figure 6, AdWnt10b transfection up-regulated the protein expression of β-catenin in the DPCs (P < 0.0001). Treatment with XAV-939, which is a specific inhibitor of Wnt/β-catenin signaling that promotes the degradation of β-catenin to block the Wnt/β-catenin signaling pathway [21], could reduce the β-catenin protein expression in DPCs (P < 0.0001).…”

Section: Wnt10b Activates the Wnt/β-catenin Pathwaymentioning

confidence: 99%

Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

Zhu

Liu

et al. 2020

Bioscience Reports

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Wnt signaling plays an important role in the growth and development of hair follicles (HFs). Among the signaling molecules, Wnt10b was shown to promote the differentiation of primary skin epithelial cells toward the hair shaft and inner root sheath of the HF cells in mice in vitro. Whisker HFs were isolated from Rex rabbits and cultured in vitro to measure hair shaft growth. Meanwhile, dermal papilla cells (DPCs) were isolated and cultured in vitro. Treatment with AdWnt10b or the Wnt/β-Catenin Pathway inhibitor, XAV939, assessed the DPCs proliferation by CCK-8 assay. And the cell cycle was also analyzed by flow cytometry. We found that Wnt10b could promote elongation of the hair shaft, whereas XAV-939 treatment could eliminated this phenomenon. AdWnt10b treatment promoted the proliferation and induced G1/S transition of DPCs. AdWnt10b stimulation up-regulated β-Catenin protein in DPCs. Inhibition of Wnt/β-Catenin signaling by XAV-939 could decreased the basal and Wnt10b-enhanced proliferation of DPCs. And could also suppress the cell cycle progression in DPCs. In summary, our study demonstrates that Wnt10b could promote HFs growth and proliferation of DPCs via the Wnt/β-Catenin signaling pathway in Rex rabbits.

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Section: Wnt10b Activates the Wnt/β-catenin Pathwaymentioning

confidence: 99%

Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

Zhu

Liu

et al. 2020

Bioscience Reports

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“…Investigation of EGF in the hair cycle generated conflicting data, since P.J. Miettinen et al, H. Zhang et al assumed EGF importance in hair follicle development and hair growth (Miettinen, 1995;Zhang et al, 2016a;Zhang et al, 2016b). Kashiwagi et al, Richardson et al showed dose and time dependent EGF effect on hair growth, using an animal model (Kashiwagi et al, 1997;Richardson, 2009).…”

Section: Discussionmentioning

confidence: 99%

A Randomized Study of Biomimetic Peptides Efficacy and Impact on the Growth Factors Expression in the Hair Follicles of Patients with Telogen Effluvium

2018

J App Pharma Sci

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Currently, biomimetic peptides have been developed to treat alopecia, which consists of amino acid residues and are able to selectively interact with cell receptors. This article studies the expression of growth factors (VEGF, KGF, EGF, TGF-β1) in the hair follicle of patients with telogen effluvium and healthy individuals and evaluating the efficacy of therapy with biomimetic peptides in patients with telogen effluvium based on clinical data and the dynamics. The method of immunofluorescence study of scalp specimens, which is taken by the punch biopsy from the frontotemporal areas before and after the therapy, was used. 30 female patients with telogen effluvium aged between 20 and 56 years old were recruited for the research and randomly divided into two groups. Patients of group I were treated with lotion based on biomimetic peptides and those of group II were treated with 0.9% saline. The therapeutic efficacy of biomimetic peptides in women with telogen effluvium was assessed by trichoscopy and phototrichography. A change in the expression of VEGF, KGF, TGF-β1 growth factors was found in women with telogen effluvium as opposed to the healthy ones. After the therapy, a significant increase in the VEGF, EGF expression and a decrease in KGF and TGF-β1 expression were noted. The study proved the effectiveness of the proposed method of therapy for women with telogenic effluvium. The research findings can be useful to dermatologists and cosmetologists for increasing the effectiveness of therapy for patients with telogenic effluvium. Also, the data obtained in the article open up the prospect for the further study of the pathogenetic mechanisms of telogenic effluvium and provide an opportunity for an innovative approach to the therapy of patients with telogenic effluvium.

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“…Эти факторы роста оказывают свое действие на волосяной фолликул аутокринно или паракринно. Стоит отметить, что большинство исследований по изучению влияния факторов роста на цикл волосяного фолликула проводились с отдельными факторами роста, в то время как в организме они работают в синергии друг с другом [33][34][35][36]. Таким образом, актуально проведение исследований различных сочетаний факторов роста по установлению их способности участвовать в развитии выпадения волос.…”

Section: Discussionunclassified

Changes in the hair growth cycle in women with non-scarring alopecia

Кубанов

Gallyamova

Галлямова³

et al. 2018

Vestnik dermatologii i venerologii

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One of the key elements in the pathophysiological process of androgenetic alopecia and telogen hair loss is the change of hair cycle. Growth factors controlling the development and cycle of the hair follicle have thus far been established. However, the role of growth factors in the pathogenesis of alopecia remains to be revealed. Objective. This study was aimed at investigating the expression of the VEGF, KGF, EGF and TGF-β1 growth factors in women with androgenetic alopecia and telogen hair loss, as well as their role in the development of alopecia. Materials and methods. 60 female patients diagnosed with telogen hair loss (30 women) and androgenetic alopecia (30 women) were observed. In order to investigate the expression of the VEGF, KGF, EGF and TGF-β1 growth factors, we conducted an immunofluorescent analysis of skin samples obtained by punch biopsy (4 mm) from the frontoparietal scalp area of patients with androgenetic alopecia and telogen hair loss. 15 samples obtained from healthy people were used as a reference group. Results. A change in the expression of the VEGF, KGF and TGF-β1 growth factors in women with androgenetic alopecia and telogen hair loss was established in comparison with healthy individuals. A correlation was found between the expression of the growth factors under study, age (p ≤ 0.05), as well as the character and duration of the disease (p ≤ 0.05) in women with non-scarring alopecia. The expression of the growth factors is found to be dependent on the clinical form of alopecia (p < 0.001). Conclusion. The VEGF growth factor is established to have the most significant effect on the development of androgenetic alopecia in women, with the KGF, TGF-β1 and EGF factors being less significant as the predictors of this disorder. The VEGF growth factor is shown to affect telogen hair loss to a greater extent compared to the EGF factor. Our study confirms differences in the pathogenesis of androgenetic alopecia and telogen hair loss in women. The findings suggest that the VEGF and KGF growth factors, as well as TGF-β1 inhibitors may be used as potential pharmacological agents for treating patients suffering from androgenetic alopecia and telogen hair loss.

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Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling

Cited by 47 publications

References 28 publications

Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

Wnt10b promotes hair follicles growth and dermal papilla cells proliferation via Wnt/β-Catenin signaling pathway in Rex rabbits

A Randomized Study of Biomimetic Peptides Efficacy and Impact on the Growth Factors Expression in the Hair Follicles of Patients with Telogen Effluvium

Changes in the hair growth cycle in women with non-scarring alopecia

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