Epidemiology of antimicrobial resistant Campylobacter spp. isolated from retail meats in Canada

Narváez‐Bravo, Claudia; Taboada, Eduardo N.; Mutschall, Steven K.; Aslam, Mueen

doi:10.1016/j.ijfoodmicro.2017.04.019

Cited by 51 publications

(46 citation statements)

References 24 publications

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“…However, we did not isolate any C. jejuni or detect C. jejuni DNA from ground beef generated from regions of carcasses (i.e., brisket and rump) that were deemed to be positive for the bacterium [30]. Considerable research has examined retail beef for C. jejuni, and they have concluded that viable C. jejuni cells are uncommon in retail beef in Canada and elsewhere [18][19][20]58,[62][63][64][65][66]. This contrasts with the high prevalence of contamination of retail poultry by C. jejuni [67].…”

Section: Discussionmentioning

confidence: 82%

“…Furthermore, C. jejuni isolates longitudinally recovered from carcasses were equally resistant to tetracycline. In all of the C. jejuni isolates recovered from cattle that we examined, resistance was attributed to carriage of tetO, which is the most common determinant conferring tetracycline resistance in the bacterium associated with cattle in Canada [18,25]. One possibility is that resistant bacteria were transmitted from cattle administered AS700 to control animals maintained in pens in proximity to one another.…”

Section: Discussionmentioning

confidence: 99%

“…The degree to which the consumption of beef contaminated with viable C. jejuni contributes to disease in SWA and elsewhere is currently unknown. A number of studies have conducted snapshot examinations of C. jejuni associated with carcasses [14][15][16][17] and retail beef [18][19][20][21], but few have longitudinally examined the transmission of C. jejuni subtypes throughout the beef production continuum to date, or examined the risk posed to people. Our research and that of others has shown that only a subset of C. jejuni subtypes are associated with human infections.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tetracycline Resistant Campylobacter jejuni Subtypes Emanating from Beef Cattle Administered Non-Therapeutic Chlortetracycline are Longitudinally Transmitted within the Production Continuum but are Not Detected in Ground Beef

et al. 2019

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The impacts of the antimicrobial growth promoter (AGP), chlortetracycline with sulfamethazine (AS700), on the development of antimicrobial resistance and longitudinal transmission of Campylobacter jejuni within the beef production continuum were empirically determined. Carriage of tetracycline resistance determinants in the enteric bacterial community increased at a greater rate for AS700-treatment cattle. The majority of the bacteria from animals administered AS700 carried tetW. Densities of C. jejuni shed in feces increased over the confined feeding period, and the administration of AS700 did not conspicuously reduce C. jejuni densities in feces or within the intestine. The majority of C. jejuni isolates recovered were resistant to tetracycline, but the resistance rates to other antibiotics was low (≤20.1%). The richness of C. jejuni subtypes recovered from AS700-treated animals that were either resistant or susceptible to tetracycline was reduced, indicating selection pressure due to AGP administration. Moreover, a degree of subtype-specific resistance to tetracycline was observed. tetO was the primary tetracycline resistance determinant conferring resistance in C. jejuni isolates recovered from cattle and people. Clinically-relevant C. jejuni subtypes (subtypes that represent a risk to human health) that were resistant to tetracycline were isolated from cattle feces, digesta, hides, the abattoir environment, and carcasses, but not from ground beef. Thus, study findings indicate that clinically-relevant C. jejuni subtypes associated with beef cattle, including those resistant to antibiotics, do not represent a significant foodborne risk.

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Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tetracycline Resistant Campylobacter jejuni Subtypes Emanating from Beef Cattle Administered Non-Therapeutic Chlortetracycline are Longitudinally Transmitted within the Production Continuum but are Not Detected in Ground Beef

et al. 2019

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“…The two PBGs, cam P and mec A ( Campylobacter and Staphylococcus , respectively), were also detected to be co‐present with all ARGs except tet A. This result is supported by the continuous identification of Staphylococcus and Campylobacter resistant to various antibiotics, such as β‐lactam, ciprofloxacin, and erythromycin (Narvaez‐Bravo, Taboada, Mutschall, & Aslam, ; Post et al., ; Qekwana, Oguttu, Sithole, & Odoi, ).…”

Section: Resultsmentioning

confidence: 76%

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

Yuan

Zhai

Mao

et al. 2019

Water Environment Research

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This study explores the prevalence, emission, and reduction of five ARGs (sulI, tetA, mphB, qnrD, and mcr‐1) and integron (intI) through a distributed swine wastewater purification facility and the effluent‐receiving environment. Typical metal resistance genes (MRGs), pathogenic bacterial indicators, the bacterial community, and wastewater properties were also explored to determine their effects on the fates of ARGs. Results indicated that the purification process could hardly effectively remove ARGs’ prevalence. 3.1 × 104–7.1 × 108 copies/L were present after purification, and 4%–57% of them persisted in the subsequent creek and adjacent soil. 16S rRNA sequencing suggested that the discharge of wastewater significantly changed the bacterial community in receiving creek and soil. Molecular ecological networks analysis detected the wide co‐occurrence among ARGs, MRGs, and PBGs, which could further facilitate the propagation of antibiotic resistance. ARG incidence and specific bacterial genera were closely correlated, suggesting an extensive hosting relationship. Redundancy analyses showed wastewater organics and nutrients showed positive correlation to most ARGs’ abundance, but negatively correlated to their relative abundance. Practitioner points Fate of five ARGs and intI was studied in a swine wastewater treatment system. The treatment process could not effectively reduce ARGs’ abundance. ARGs and pathogens in wastewater were transferred to the receiving creek and soil. The network analysis found wide co‐occurrence among ARGs, metal resistance genes, and pathogens. Wastewater nutrients positively correlated to ARG's abundance but negatively correlated to their relative abundance.

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“…The antibiotic resistance pro le in this study was almost identical to the ndings of Rajagunalan (19) who observed C. jejuni to be 100% sensitive to gentamicin, ampicillin and erythromycin and 100% resistant to cephalothin and co-trimoxazole. Narvaez et al (67) reported that 71.4% of Campylobacter isolates had sensitivity against nalidixic acid followed by tetracycline (48.1%), cipro oxacin (5.5%), azithromycin (1.78%) and erythromycin (1.78%). All isolates were susceptible to clindamycin, orfenicol, gentamicin and telithromycin and tetracycline resistance was attributable to the presence of the tet(O) gene.…”

Section: Phenotypic Antimicrobial Susceptibilitymentioning

confidence: 99%

Characterization of isolated thermophilic campylobacters and associated risk factors in poultry farms of Uttarakhand, India

Garhia¹,

Mani²,

Kumar³

et al. 2020

Preprint

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Background Campylobacters are the common commensals of poultry responsible for several cases of gastroenteritis in humans. The illness, if severe can result into complications causing a nervous disorder named Guillian Barre syndrome. Owing to its serious health implications, the study aimed to screen eight organized poultry farms and their environment (water, litter, manure, and feed) of Uttarakhand state, India for the presence of thermophilic Campylobacter species and their virulence and antibiotic resistance profile. It also undertook identification of risk factors associated with the occurrence of campylobacters in each farm using a questionnaire survey comprising eleven potential risk factors (other animals on farm, reuse of litters, use of foot bath, in house or branded feed, chlorination of water, distance of manure heap, housing system, flock size, floor type, shoe use by farm personnels, moist or dry litter and number of broiler floor). Results Of eight, six farms showed varying occurrence of C.jejuni and C.coli with an overall prevalence of 12.29%. Not a single isolate of C.lari and C.upsaliensis was recorded. Poultry faecal, water and litter samples observed 18.2%, 6% and 1.9% presence, respectively. Feed and manure samples did not appear positive. In 48 revived Campylobacter isolates, 100% presence of cadF and flaA virulence genes were detected followed by cdtB (97.9%), cgtB (22.9%) and ciaB (12.5%), respectively. Ten isolates 23.80%) were multidrug resistant (MDR) exhibiting resistance to at least 3 or more antimicrobial classes. The most common MDR patterns were AMP CX CIP TE (n = 2) and AMP CX CIP (n = 2). Feeding of branded feed was found to have significant association with Campylobacter presence in the examined broiler flocks (p-value 0.0047). Conclusions The study highlights the occurrence of food pathogens, Campylobacter jejuni and C.coli in the poultry farms and their environment of the state. The organisms possessed significant virulence genes capable of developing critical human illness. Overall, the presence of MDR thermophilic campylobacters appears to be a severe public threat.

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Epidemiology of antimicrobial resistant Campylobacter spp. isolated from retail meats in Canada

Cited by 51 publications

References 24 publications

Tetracycline Resistant Campylobacter jejuni Subtypes Emanating from Beef Cattle Administered Non-Therapeutic Chlortetracycline are Longitudinally Transmitted within the Production Continuum but are Not Detected in Ground Beef

Tetracycline Resistant Campylobacter jejuni Subtypes Emanating from Beef Cattle Administered Non-Therapeutic Chlortetracycline are Longitudinally Transmitted within the Production Continuum but are Not Detected in Ground Beef

Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant

Characterization of isolated thermophilic campylobacters and associated risk factors in poultry farms of Uttarakhand, India

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