Epidemiology and phospholipase activity of oral Candida spp. among patients with central nervous system diseases before and after dental cleaning procedure

Abstract

Patients suffering of diseases that affect central nervous system may be considered more susceptible to the infectious diseases of mouth. Sixty-nine patients suffering of cerebral palsy, Down’s syndrome and metal retardation were submitted to saliva examination for the presence of Candida spp. before and after a procedure of dental cleaning. The isolates were submitted to assay for verifying phospholipase production. 55.10% of the patients provided isolation of Candida spp. The frequency of isolation obtained before dental procedure was: C. albicans (83.33%), C. krusei (8.33%) and C. kefyr, C. parapsilosis and C. glabrata (2.78% each). The frequency after the procedure was: C. albicans (68.57%), C. parapsilosis (11.43%), C. krusei and C. kefyr (8.57% each) and Candida glabrata (2.86%). We verified significantly difference (p < 0.01) between populations obtained at the two examinations. Phospholipase production was verified only among C. albicans strains and the proportion of producers was higher when testing isolates obtained after dental cleaning procedure. Studies focused on Candida spp. isolation are useful for better comprehension of the role of these yeasts on the oral flora from patients with cerebral palsy, Down’s syndrome and metal retardation.

Novel Preclinical Study of Galloylquinic Acid Compounds from Copaifera lucens with Potent Antifungal Activity against Vaginal Candidiasis Induced in a Murine Model via Multitarget Modes of Action

“…Briefly, 100 μL of serially diluted GQAs (13.87 to 222 μg/mL) was incubated with precultured Vero cells (6 × 10 4 cells/mL) in Dulbecco’s modified Eagle medium (DMEM) using 96-well culture plate. Following 48 h of incubation, the cytotoxic effects of GQAs on test cells were determined quantitatively using the MTT assay method as described by Vistica et al ( 44 ) and Ribeiro et al ( 45 ).…”

“…Phospholipase activity (P z ) was determined by measuring the ratio of colony diameter to the total diameter of both the colony and the precipitation zone. A P z value of <1 indicates enzymatic activity, while a value of 1 denotes no activity; i.e., the lower the value of P z , the higher the phospholipase activity ( 45 ).…”

Inhibitory Effect of Morin Against Candida albicans Pathogenicity and Virulence Factor Production: An in vitro and in vivo Approaches

“…For qualitative analysis of phospholipase production, 1 µl of inoculum was placed at the center of the SDA plate containing 1% peptone, 3% glucose, 5.73% NaCl, 0.055% CaCl 2 , and 10% of egg yolk emulsion in the absence and presence of morin (at MBIC), and the plates were then incubated at 37 • C for 72 h (Ribeiro et al, 2010). The diameter of the precipitation zone around the colony was determined.…”