Epidemiology and molecular characterization of multidrug-resistant &lt;em&gt;Escherichia coli&lt;/em&gt; isolates harboring &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;CTX-M&lt;/sub&gt; group 1 extended-spectrum &amp;beta;-lactamases causing bacteremia and urinary tract infection in Manhi&amp;ccedil;a, Mozambique

Guiral, Elisabet; Pons, María J.; Vubil, Delfino; Marí-Almirall, Marta; Sigaùque, Betuel; Soto, Sara M.; Alonso, Pedro L.; Ruiz, Joaquı́m; Vila, Jordi; Mandomando, Inácio

doi:10.2147/idr.s153601

Cited by 29 publications

(22 citation statements)

References 47 publications

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“…In the study of Haba Maged (2018), TEM, SHV and CTX-M genes were found in 42.8%, 36.7% and 38.6% of cases, respectively [17]. According to the study by Elizabeth Gaviral (2018), 92% of ESBL-containing strains had the CTX-M 15 gene and the SHV gene was found in only two isolates, which indicates a high degree of consistent with this study [18]. In the Abdulaziz Qassim (2018) study, 93.94% of the strains also contained the CTX-M gene, and TEM was found in 12.12% of the strains, which is opposed with the results of this study regarding the TEM gene [7].…”

Section: Discussionsupporting

confidence: 75%

Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

Namaei¹,

Hamzei²,

Moghanni³

et al. 2020

Preprint

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Background: Urinary Tract Infection (UTI) is the most common bacterial infection in the world. E. coli is the predominant Pathogen. This study evaluates the prevalence of ESBL in E. colis isolated from patients with urinary tract infections with phenotypic and genotypic methods.Methods: This descriptive-analytical study was done on 155 isolates of E. coli isolated from patients with urinary tract infection who had received the study consent. After accurate identification of E. coli strains. ESBL production for Escherichia coli isolates which are resistant to ceftriaxone or ceftazidime was evaluated by CDT method. TEM, SHV and CTX-M genes were identified by PCR.Results: The results showed that 30 strains from 155 strains of E. coli had ESBL. Strains of ESBL producer were more in males was lower in educated persons. 38.9% of ESBL producer had antibiotic use, 29.9% -producing Escherichia hospitalization and 31.6% uti history. The highest level of drug allergy in the ESBL was related to nitrofurantoin, and the highest resistance was related to cefazolin, co-trimoxazole. The CTX-M and the CTX-M15 gene were found in 92.7% and 57.1% of cases, respectively; also the SHV and TEM genes were not found in any of ESBL-producing Escherichia coli strains. Most therapeutic response in patients was related to cefexime, ciprofloxacin and nitrofurantoin 27.4%, 26% 21.9%, respectively.Conclusion: This study showed that the history of antibiotic use, hospitalization, uti related to increase of ESBL-producing in E. coli isolates., the CTMX-M gene is the most common gene in ESBL-producing E. coli strains.

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Section: Discussionsupporting

confidence: 75%

Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

Namaei¹,

Hamzei²,

Moghanni³

et al. 2020

Preprint

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“…The integration of mobilizable antibiotic resistance genes within the bacterial chromosome has been largely described. Thus, many transferable resistance genes, including those encoding ␤-lactamases, aminoglycoside-modifying enzymes, or trimethoprim resistance determinants, have been detected in the chromosomes of different microorganisms (141,(185)(186)(187)(188)(189)(190)(191)(192)(193)(194)(195)(196). With regard to TMQR, this finding has also been described.…”

Section: Nomenclature Of the Transferable Mechanisms Of Quinolone Resmentioning

confidence: 90%

“…The risks of the rising levels of antimicrobial resistance worldwide have been increasingly discussed and highlighted (128)(129)(130), with subsequent proposals for the implementation of different actions (128,131,132). Nevertheless, in the present century, the continuously rising global trend toward the isolation of pathogenic or nonpathogenic quinolone-resistant microorganisms has remained unaltered (55,113,(133)(134)(135)(136)(137)(138)(139)(140) and has expanded to regions with limited access to antibiotic agents (141)(142)(143). In fact, the use of quinolones has been compromised in different areas because of the high percentages of quinolone resistance among specific pathogens (134,(144)(145)(146)(147).…”

Section: Evolution Of Quinolone Resistance Over Timementioning

confidence: 99%

Transferable Mechanisms of Quinolone Resistance from 1998 Onward

Ruiz

2019

Clin Microbiol Rev

Self Cite

115

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SUMMARY While the description of resistance to quinolones is almost as old as these antimicrobial agents themselves, transferable mechanisms of quinolone resistance (TMQR) remained absent from the scenario for more than 36 years, appearing first as sporadic events and afterward as epidemics. In 1998, the first TMQR was soundly described, that is, QnrA. The presence of QnrA was almost anecdotal for years, but in the middle of the first decade of the 21st century, there was an explosion of TMQR descriptions, which definitively changed the epidemiology of quinolone resistance. Currently, 3 different clinically relevant mechanisms of quinolone resistance are encoded within mobile elements: (i) target protection, which is mediated by 7 different families of Qnr (QnrA, QnrB, QnrC, QnrD, QnrE, QnrS, and QnrVC), which overall account for more than 100 recognized alleles; (ii) antibiotic efflux, which is mediated by 2 main transferable efflux pumps (QepA and OqxAB), which together account for more than 30 alleles, and a series of other efflux pumps (e.g., QacBIII), which at present have been sporadically described; and (iii) antibiotic modification, which is mediated by the enzymes AAC(6′)Ib-cr, from which different alleles have been claimed, as well as CrpP, a newly described phosphorylase.

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“…Escherichia coli displaying phenotypic ESBL activity were tested for the presence of SHV, TEM, OXA-1-like, CTX-M-2, CTX-M-3, CTX-M-8, CTX-M-9, and CTX-M-10 genes using conventional polymerase chain reaction. 22 Identified ESBL genes were not sequenced for allelic variants.…”

Section: Methodsmentioning

confidence: 99%

Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca, Peru

Larson

Hartinger

Riveros

et al. 2019

The American Journal of Tropical Medicine and Hygiene

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Antibiotic resistance in pathogenic bacteria is a serious public health issue. The growing threat is a cause for concern and action to prevent the emergence of new resistant strains and the spread of existing ones to humans via the environment. This study aimed at identifying fecal pathogens in drinking water obtained from rural Andean households from Cajamarca, Peru, and measuring the antibiotic resistance profile of Escherichia coli. The study was embedded within a community-randomized controlled trial among 102 communities in the northern highlands of the Cajamarca region, Peru. Of 314 samples, 55.4% (95% CI [49.7, 61.0], n = 174) were identified as thermotolerant coliforms. Among the samples positive for thermotolerant coliform, E. coli was isolated in 37.3% (n = 117), Klebsiella spp. in 8.0% (n = 25), Enterobacter spp. in 5.1% (n = 16), and Citrobacter spp. in 2.5% (n = 8). Of the 117 E. coli samples, 48.7% (95% CI [39.4, 58.1], n = 57) showed resistance to any antibiotic. The E. coli antibiotic resistance profile showed highest resistance against tetracycline (37.6%), ampicillin (34.2%), sulfamethoxazole-trimethoprim (21.4%), and nalidixic acid (13%). Some 19.7% (95% CI [12.9, 28.0], n = 23) of the E. coli isolates displayed multidrug resistance, defined as resistance to at least three classes of antibiotics. The CTX-M-3 gene, which encodes extended-spectrum resistance to beta-lactamase antibiotics, was found in one isolate. The high prevalence of fecal contamination in drinking water highlights the importance of household water treatment methods. Likewise, the high levels of antibiotic resistance found indicate a need for further research to identify the origins of potential environmental contamination, misuse, or inadequate disposal of antibiotics.

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Epidemiology and molecular characterization of multidrug-resistant <em>Escherichia coli</em> isolates harboring <em>bla</em><sub>CTX-M</sub> group 1 extended-spectrum β-lactamases causing bacteremia and urinary tract infection in Manhiça, Mozambique

Cited by 29 publications

References 47 publications

Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

Transferable Mechanisms of Quinolone Resistance from 1998 Onward

Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca, Peru

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