Enzymology and Molecular Biology of Lignin Degradation

Cullen, Daniel; Kersten, Philip J.

doi:10.1007/978-3-662-10367-8_13

Cited by 45 publications

(45 citation statements)

References 205 publications

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“…2 and Table 2), the connective modules of both carbohydrateactive enzymes (CAZys; especially cellulose-binding region) and the ligninolytic degraders, ureidoglycolate hydrolase as a glyoxalate generator, as the hydrolysable triggers, were responsible for the optimized metabolic conversion of pre-processed RS into a fermentable carbon chain and ethanol. Remarkably, the expression level of CAZys, namely β-glucosidase, CDH, and hemicellulolytic xylanase, which are target factors for the practical downstream index, and peroxidative enzymes, including copper amine oxidase, in WSMB had a very low rate (fold <2) of intracellular activities in the context of cellulolytic mechanism, unlike previously identified microbial biosystems [26,33,34]. This difference is likely due to the platform being optimized for extracting cellulosic microfibers, which is based on the ligninolytic cascade via target optimization, especially by MnP.…”

Section: Multiple Target-specific Deconstruction Of Open Cellulosic Mcontrasting

confidence: 58%

“…a Untreated RS. b RS biopretreated by optimal WSMB for 12 days environmentally evolutionary process of survival [25,26], suggesting that, remarkably, the effective yields of the bio-pretreatment platform may be involved in the proactive or interconnective regulation of upstream metabolic triggers, especially lignocellulolysis-induced signals.…”

Section: Proteomic Evaluation Of Advanced Wsmb Biosystem: Nonspecificmentioning

confidence: 99%

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Bioprocess Evaluation of Water Soaking-Based Microbiological Biodegradation with Exposure of Cellulosic Microfibers Relevant to Bioconversion Efficiency

Bak

2015

Appl Biochem Biotechnol

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To verify the interconnective relationship between biodegradation efficiency and microfibril structure, recalcitrant rice straw (RS) was depolymerized using water soaking-based microbiological biodegradation (WSMB). This eco-friendly biosystem, which does not predominantly generate inhibitory metabolites, could increase both the hydrolytic accessibility and fermentation efficiency of RS. In detail, when swollen RS (with Fenton cascades) was simultaneously bio-treated with Phanerochaete chrysosporium for 12 days, the biodegradability was 65.0 % of the theoretical maximum at the stationary phase. This value was significantly higher than the 30.3 % measured from untreated RS. Similarly, the WSMB platform had an effect on the yield enhancement of ethanol productivity of 32.5 %. However, uniform exposure of fibril polymers appeared to have little impact on bioconversion yields. Additionally, the proteomic pools of the WSMB system were analyzed to understand either substrate-specific or nonspecific biocascades based on the change in microcomposite materials. Remarkably, regardless of modified microfibril chains, the significant pattern of 14 major proteins (|fold| > 2) was reasonably analogous in both systems, especially for lignocellulolysis-related targets.

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Section: Multiple Target-specific Deconstruction Of Open Cellulosic Mcontrasting

confidence: 58%

Section: Proteomic Evaluation Of Advanced Wsmb Biosystem: Nonspecificmentioning

confidence: 99%

Bioprocess Evaluation of Water Soaking-Based Microbiological Biodegradation with Exposure of Cellulosic Microfibers Relevant to Bioconversion Efficiency

Bak

2015

Appl Biochem Biotechnol

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“…Three of these putative copper radical oxidase genes (cro3, cro4, and cro5) are clustered within a larger cluster of lignin peroxidase genes (7,32).…”

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confidence: 99%

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

Wymelenberg

Sabat

Mozuch

et al. 2006

Appl Environ Microbiol

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The white rot basidiomycete Phanerochaete chrysosporium produces an array of nonspecific extracellular enzymes thought to be involved in lignin degradation, including lignin peroxidases, manganese peroxidases, and the H 2 O 2 -generating copper radical oxidase, glyoxal oxidase (GLX). Preliminary analysis of the P. chrysosporium draft genome had identified six sequences with significant similarity to GLX and designated cro1 through cro6. The predicted mature protein sequences diverge substantially from one another, but the residues coordinating copper and constituting the radical redox site are conserved. Transcript profiles, microscopic examination, and lignin analysis of inoculated thin wood sections are consistent with differential regulation as decay advances. The cro2-encoded protein was detected by liquid chromatography-tandem mass spectrometry in defined medium. The cro2 cDNA was successfully expressed in Aspergillus nidulans under the control of the A. niger glucoamylase promoter and secretion signal. The recombinant CRO2 protein had a substantially different substrate preference than GLX. The role of structurally and functionally diverse cro genes in lignocellulose degradation remains to be established.

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“…The differences in the fungal species and strains and growth conditions (composition of the culture medium, incubation time, with or without agitation) strongly affect the isoenzyme patterns produced by the different fungi (Boer et al, 2006). Purification methods and storage also can affect the relative isoenzymatic levels (Cullen and Kersten, 1996). …”

Section: Molecular Massmentioning

confidence: 99%

A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

Acevedo

Pizzul

Castillo

et al. 2011

Braz. arch. biol. technol.

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Enzymology and Molecular Biology of Lignin Degradation

Cited by 45 publications

References 205 publications

Bioprocess Evaluation of Water Soaking-Based Microbiological Biodegradation with Exposure of Cellulosic Microfibers Relevant to Bioconversion Efficiency

Bioprocess Evaluation of Water Soaking-Based Microbiological Biodegradation with Exposure of Cellulosic Microfibers Relevant to Bioconversion Efficiency

Structure, Organization, and Transcriptional Regulation of a Family of Copper Radical Oxidase Genes in the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium

A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

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