Enzymic Studies on Mannitol Formation by Piricularia oryzae***

Yamada, Hiromi; Okamoto, Koji; Kodama, K.; Noguchi, Fumiko; Tanaka, Shōzō

doi:10.1093/oxfordjournals.jbchem.a127317

Cited by 28 publications

(6 citation statements)

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“…Forination of inannitol froin F6P is catalyzed by fructose-6-phosphate reductase. Yamada et al (1961) reported the presence of this enzyme in extracts from three different fungi. I t is possible that this enzyine was also present in the rust-inoculated leaves investigated by us.…”

Section: Discussionmentioning

confidence: 99%

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Lunderstädt¹

1966

Can. J. Bot.

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The increase in activity of hexolcinase occurring in leaves of wheat (Triticum satat~nt L., variety Little Club) after infection with stem rust (P,zlccinia graminis (Pets.) f. sp. tritizi Erikss. and Henn.) cannot be attributed t o activation, or relief of inhibition, by compounds of low molecular weight in the host-parasite complex. The light regime t o which plants were subjected after inoculation was found t o modify the activity of this enzyme in crude extracts, presumably b y affecting the concentration of inhibitory substances. Fractionation of proteinaceous material by gel filtration produced evidence that infection results in qualitative differences of the hexol~inase protein: the enzyme preparation obtained from infected leaves differs in its behavior on Sephadex G-100 columns from hexokinase preparations isolated from healthy leaves, approximating the gel filtration properties of rust spore hexoltinase. The activity in vitro of invertase, glucohexolcinase, and fructohesolcinase was found t o be correlated with the concentration of their respective substrates in the host-parasite complex: after infection, a small increase of endogenous substrate concentration was accompanied by a small increase of the enzyme activity in vitro; and vice versa. Measurements of invertase activity in vitro indicated, and feeding of sucrose-U-14C t o detached leaves confirmed, that sucrose dissimilation is increased after infection with rust. As measured in crude enzyme preparations, the postinfectional increase of invertase activity was always larger than that of either glucohexokinase or fructohexol

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Section: Discussionmentioning

confidence: 99%

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Lunderstädt¹

1966

Can. J. Bot.

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“…Similar systems have been described in Candida utilis (Arcus and Edson, 1956;Chakravorty et al, 1962), C. albicans (Veiga, Bacila, and Horecker, 1960), and Penicillium chrysogenum (Chiang and Knight, 1959). In comparison, the NAD-dependent polyol dehydrogenase of Piricularia oryzae (Yamada et al, 1961) and Coccidioides immitis (Lones and Peacock, 1964) functions at the level of phosphorylated intermediates in the conversion of fructose-6-phosphate to mannitol-1-phosphate, whereas the reduction of fructose to mannitol in A. campestris (Edmundowicz and Wriston, 1963) and Geotrichum candidum (Shu-Cheng and Kao-Hsiang, 1964) requires NADPH2.…”

Section: Discussionmentioning

confidence: 53%

Polyol Metabolism in the Basidiomycete Schizophyllum commune

1965

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The polyol metabolism of intact cells and extracts of the wood-rotting mushroom Schizophyllum commune was investigated during the developmental cycle. Exogenous polyols stimulated the cellular respiration of germlings, but were without effect on that of ungerminated basidiospores. Requisite enzymes of polyol metabolism were demonstrated in extracts of spores and of subsequent stages of development. Oxidation of mannitol and sorbitol appeared to be coupled to nicotinamide adenine dinucleotide (NAD) reduction and was favored in alkaline medium. Ketohexose formation was shown during mannitol oxidation, and the NADdependent oxidation of xylitol yielded ketopentose. Xylitol oxidation with nicotinamide adenine dinucleotide phosphate (NADP) as hydrogen acceptor led to pentose formation. Oxidation of reduced NAD was enhanced by fructose but not by sorbose. Reduction of aldohexose and pentose was dependent upon reduced NADP, and pentose reductase was maximal at pH 6.8. The specific activity of mannitol dehydrogenase was highest in extracts of vegetative mycelium. Growth in either glucose or xylose media had no significant effect on enzymes of polyol metabolism.

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“…RESULTS AND DISCUSSION Yamada et al (13) reported finding both NADlinked MIP dehydrogenase and mannitol-lphosphatase in crude extracts of A. niger and A.…”

Section: Methodsmentioning

confidence: 95%

“…The crude enzyme was inactive with glucose-6-phosphate, F6P, sorbitol-6-phosphate, and mannose-6-phosphate. Since both MlP dehydrogenase and mannitol-1-phosphatase were present in the extracts from glucose-grown cells, it seemed reasonable to assume that mannitol was being synthesized via F6P reduction as in other aspergilli (4,13). However, a high level of NADP-linked mannitol dehydrogenase occurred in the extracts.…”

Section: Methodsmentioning

confidence: 99%

d -Mannitol Metabolism by Aspergillus candidus

Strandberg

1969

J Bacteriol

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Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-l-phosphate (MIP) dehydrogenase was found in French pressure cell extracts of D-glucose-grown cells, whereas a specific mannitol-l-phosphatase was present in extracts prepared by both methods. The existence of these two enzymes indicated that mannitol may be synthesized in this organism by the reduction of fructose-6phosphate. A specific nicotinamide adenine dinucleotide phosphate-linked mannitol dehydrogenase was also identified in both extracts. This enzyme may have been involved in mannitol utilization. However, the level of the mannitol dehydrogenase appeared to be substantially reduced in extracts from mannitol-grown cells, whereas the level of MIP dehydrogenase was increased. A hexokinase has been identified in this organism. Fructose-6-phosphatase, glucose isomerase, and mannitol kinase could not be demonstrated. remove cations. Mannitol and free sugars were de-1305 Vol. 97, No. 3

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Enzymic Studies on Mannitol Formation by Piricularia oryzae***

Cited by 28 publications

References 0 publications

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Effect of Rust Infection on Hexokinase Activity and Carbohydrate Dissimilation in Primary Leaves of Wheat

Polyol Metabolism in the Basidiomycete Schizophyllum commune

d -Mannitol Metabolism by Aspergillus candidus

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