Enzymic Histochemistry of Granular Components in Digestive Gland Cells of the Roman Snail, Helix Pomatia

Rosenbaum, Robert M.; Ditzion, Bruce

doi:10.2307/1539497

Cited by 28 publications

(13 citation statements)

References 19 publications

(31 reference statements)

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“…It seems likely in the case of acid phosphatase that this represents diffusion of the reaction product, but it seems probable, in the case of non-specific esterase, that there is a cytoplasmic, as well as a lysosomal, localization of the enzyme. Some of the results described here were also obtained by previous workers, Billett & McGee-Russell (1955) found /3-glucuronidase in the cytoplasm and colourless granules of digestive cell8, while Rosenbaum & Ditzion (1963) localized several hydrolytic enzymes in the cytoplasm and yellow granules, and found them associated with ingested material However, these and other workers found more restricted distributions of the enzymes in the digestive cells than have been found here, and, in particular, they did not find the cytoplasmic granules.…”

Section: Discussionsupporting

confidence: 89%

“…Nakazima (1956), using Gomori's method for acid phosphatase, found this enzyme in the calcium spherules of Euhudru. Rosenbaum & Ditzion (1963) obtained a positive reaction for acid phosphatase in the calcium spherules of Helix, using Gomori's method, but failed to obtain it with Burstone's naphthol AS method. The reasons for this discrepancy are not clear; the variability of the reactions with the different methods might be due to the specificity of the enzyme, or due to the relative impenetrability of the spherules by certain substrates.…”

Section: Discussionmentioning

confidence: 99%

“…It is well established that certain cells in the digestive glands of many molluscs ingest food particles, (Cuknot, 1899;Jordan, 1918;Yonge, 1926a,b;Millott, 1937;Rosen, 1941Rosen, , 1952Rosen, , 1965Owen, 1955;Rosenbaum & Ditzion, 1963;Sumner, 1965b), which are digested intracellularly (Rosen, 1930(Rosen, , 1941(Rosen, , 1950(Rosen, ,1952Millott, 1937). The cells concerned * Present address: Medical Research Council, Clinical and Population Cytogenics Research Unit, Western General Hospital, Crewe Road,Edinburgh,4. in this process are the digestive cells (for a partial synonymy of the cell types in the pulmonate digestive gland, see Sumner, 1965a).…”

Section: Introductionmentioning

confidence: 99%

“…Although certain acid hydrolases, especially aryl sulphatase and p-glucuronidase, have been found biochemically in the digestive glands of molluscs (Jarrige & Henry, 1952;Dodgson, Lewis & Spencer, 1953;Billett, 1954;Cox, 1959;Dodgson & Powell, 1959;Leon, Bulbrook & Corner, 1960;Orzel, 1967), such methods give no information about the localization of the enzymes within the cells. The small amount of histochemical work which has been done on the enzymes in the digestive gland (Billett & McGee-Russell, 1955;Nakazima, 1956;Pugh, 1963;Rosenbaum & Ditzion, 1963;Muller, 1965;Reid, 1966Reid, , 1968James & Bowers, 1967;Taylor, 1968) provides little evidence for a lysosomal localization of hydrolytic enzymes in the digestive cells, or for their participation in intracellular digestion.…”

Section: Introductionmentioning

confidence: 99%

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The distribution of some hydrolytic enzymes in the cells of the digestive gland of certain lamellibranchs and gastropods

Sumner

1969

Journal of Zoology

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Five hydrolytic enzymes (acid phosphatase, aryl sulphatase, β‐glucuronidase, N‐acetyl‐β‐glucosaminidase, and non‐specific esterase) have been studied histochemically in the cells of the digestive gland of Mytilus edulis, Helix aspersa, and certain other lamellibranchs and gastropods. All the enzymes studied have basically similar distributions. In the digestive cells, the enzymes occur in cytoplasmic granules which are believed to be primary lysosomes; in vacuoles which contain phagocytosed food material; and in vacuoles containing lipofuscin granules, which are the residues of digestive activity. In the basiphil cells of M. edulis, most of the enzymes are localized in a few cytoplasmic granules; non‐specific esterase, however, is found throughout the cytoplasm. In the calcium cells of H. aspersa and the other pulmonate gastropods studied, the enzymes are either in cytoplasmic granules, or distributed diffusely throughout the cytoplasm. Acid phosphatase is also found in the calcium spherules, especially in H. aspersa. In the excretory cells of H. aspersa and the other pulmonates studied, the enzymes are found in granules in the cytoplasm, and in the lipofuscin granules which lie in the vacuoles of these cells.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The distribution of some hydrolytic enzymes in the cells of the digestive gland of certain lamellibranchs and gastropods

Sumner

1969

Journal of Zoology

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“…Sections cut at 7-8 p were used for tracing the fate of ingested yeast cells and also for identification and localization of a number of enzymes concerned in digestion. A positive reaction to the Hess & Pearse (1958) method for cathepsin C-like esterase has been interpreted in previous studies by Jennings (1962a,b), Rosenbaum & Ditzion (1963) and Jennings & Mettrick (1968) as a demonstration of endopeptidase activity, since it coincided with observed progressive proteolysis in the gut of various invertebrates. In the absence of more specific techniques for endopeptidases this method was used in the present study, using o-acetyl-5-bromoindoxyl acetate as substrate (Holt & Withers, 1952).…”

Section: Methodsmentioning

confidence: 66%

Feeding and digestion in Dinophilus gyrociliatus (Annelida: Archiannelida)

Jennings

Gelder

1969

Journal of Zoology

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The littoral archiannelid Dinophilus gyrociliatus Schmidt 1857 is microphagous and feeds on bacteria, protozoa, unicellular algae, diatoms and organic detritus. Two types of feeding mechanisms are used and both are relatively unselective. In the first, suspended particles brought to the head in water currents set up by the beating of transverse ciliated bands are trapped in mucus and swept laterally and ventrally on to the under surface of the head, where further ciliary activity conveys them posteriorly to the mouth. In the second feeding method the proboscis dislodges microscopic food from the sub‐stratum and freed particles are collected by ventral cilia and passed into the mouth. Mucus discharged into the mouth facilitates ingestion and other non‐mucoid secretions are poured on to the food as it passes from the oesophagus into the proventriculus where it accumulates. The proboscis is then periodically thrust up the oesophagus to drive the accumulated material onwards into the stomach. Digestion is entirely extracellular. Proteolysis initiated in the stomach by endopeptidases secreted by acidophilic glands in the stomach wall is completed in the posterior region of the stomach and in the intestine by exopeptidases produced by other components of the gut epithelium. Acid phosphatase activity is present throughout the gut epithelium at all times, irrespective of the nutritive state of the animal, but alkaline phosphatase can be demonstrated only whilst food is present in the gut. This enzyme is confined to a thin distal band below the cilia of the stomach and intestine, and reaches maximum activity in the intestinal wall 18 h after feeding, indicating that it is concerned with absorption of the products of digestion. Fat deposits occur in the stomach wall and constitute the main food reserve. Amyloid granules are laid down subepidermally in the anterior region of the body and there is some evidence that these too are utilized during periods of food shortage.

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Histochemical studies on cell death and histolysis during regeneration I. Distribution of acid phosphomonoesterase activity in the normal, the regenerating and the resorbing forelimb of the larval spotted salamander, Amblystoma maculatum

Weiss

Rosenbaum

1967

Journal of Morphology

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Histochemical methods, especially azo dye methods for detecting acid phosphomonoesterase activity were applied to normal, regenerating and denervated, amputated limbs from larval Amblystoma maculatum. Efforts were made to control inactivation of enzymic activity and diffusion of both enzyme and reaction product. "Base-line'' values for enzymic activity were determined for normal limbs. Activity appeared most intense in macrophages, less intense in epidermis and cartilage matrix. Some activity was detected in Schwann cells, peri-and endoneurium and muscle fibers from normal limbs. Enzymic activity in regenerating limbs was strongest within macrophages which appeared in increased numbers especially in early stages. Wound tissue showed little increased activity. As the blastema formed, increased enzymic activity was detected in epidermis and within increased numbers of macrophages. Chondrocytes showed increased activity especially during cartilage matrix deposition. Amputated, denervated limbs showed large numbers of active macrophages beneath and within epidermis and along muscle. As regression commenced, areas of cartilage matrix breakdown showed increased enzymic activity but, in general, greatest activity was in macrophages. The various possible roles of acid phospbomonoesterase activity in the specific biological situations dealt with are considered in light of such observations.

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Enzymic Histochemistry of Granular Components in Digestive Gland Cells of the Roman Snail, Helix Pomatia

Cited by 28 publications

References 19 publications

The distribution of some hydrolytic enzymes in the cells of the digestive gland of certain lamellibranchs and gastropods

The distribution of some hydrolytic enzymes in the cells of the digestive gland of certain lamellibranchs and gastropods

Feeding and digestion in Dinophilus gyrociliatus (Annelida: Archiannelida)

Histochemical studies on cell death and histolysis during regeneration I. Distribution of acid phosphomonoesterase activity in the normal, the regenerating and the resorbing forelimb of the larval spotted salamander, Amblystoma maculatum

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