Enzyme recovery by liquid-liquid extraction using reversed micelles

Liquid-Liquid Extraction of Proteins with Reversed Micelles

“…Participation of electrostatic interactions in phase transfer is supported by the narrowing of the pH peaks when the ionic strength is increased, as observed for the extraction of cytochrome c (Göklen, 1986). These results, found with anionic surfactant AOT, are consistent with the phase‐transfer behavior of α‐amylase (Dekker et al, 1986) in a system including TOMAC, a cationic surfactant.…”

Liquid-Liquid Extraction of Proteins with Reversed Micelles

“…Participation of electrostatic interactions in phase transfer is supported by the narrowing of the pH peaks when the ionic strength is increased, as observed for the extraction of cytochrome c (Göklen, 1986). These results, found with anionic surfactant AOT, are consistent with the phase‐transfer behavior of α‐amylase (Dekker et al, 1986) in a system including TOMAC, a cationic surfactant.…”

Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A

“…Similar influence was also reported in the recycling of other affinity-based reversed micelles [36]. In contrast, the loss of ionic surfactant in extraction processes was considered as the main reason for the decrease of protein extraction yield in the recycling of conventional ionic surfactant-based reversed micelles [37]. In the CB-Span 85 reversed micellar system, Span 85 molecules of low HLB value (1.8) have a high partition coefficient to the organic phase, so the amount of Span 85 lost in the aqueous phase was little in the extraction process.…”

Nondispersive membrane solvent back extraction of Phenol

“…While working with the organic phase on the tube side, if the organic phase pressure exceeded 1.72-3.45 kPa the organic phase would go into the micropores of the hydrophilic MHFs. Dekker et al (1987) have reported penetration of water into the micropores for large pore (0.1 pm) hydrophobic polypropylene fibers. This behavior most likely explains the tube-side experimental data for N , , for hydrophilic module 2 shown in Figure 1 1 .…”

Liquid-Liquid Extraction of Proteins with Reversed Micelles

“…Participation of electrostatic interactions in phase transfer is supported by the narrowing of the pH peaks when the ionic strength is increased, as observed for the extraction of cytochrome c (Göklen, 1986). These results, found with anionic surfactant AOT, are consistent with the phase‐transfer behavior of α‐amylase (Dekker et al, 1986) in a system including TOMAC, a cationic surfactant.…”

Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A

“…Similar influence was also reported in the recycling of other affinity-based reversed micelles [36]. In contrast, the loss of ionic surfactant in extraction processes was considered as the main reason for the decrease of protein extraction yield in the recycling of conventional ionic surfactant-based reversed micelles [37]. In the CB-Span 85 reversed micellar system, Span 85 molecules of low HLB value (1.8) have a high partition coefficient to the organic phase, so the amount of Span 85 lost in the aqueous phase was little in the extraction process.…”

Nondispersive membrane solvent back extraction of Phenol

“…While working with the organic phase on the tube side, if the organic phase pressure exceeded 1.72-3.45 kPa the organic phase would go into the micropores of the hydrophilic MHFs. Dekker et al (1987) have reported penetration of water into the micropores for large pore (0.1 pm) hydrophobic polypropylene fibers. This behavior most likely explains the tube-side experimental data for N , , for hydrophilic module 2 shown in Figure 1 1 .…”

Liquid-Liquid Extraction of Proteins with Reversed Micelles

“…Participation of electrostatic interactions in phase transfer is supported by the narrowing of the pH peaks when the ionic strength is increased, as observed for the extraction of cytochrome c (Göklen, 1986). These results, found with anionic surfactant AOT, are consistent with the phase‐transfer behavior of α‐amylase (Dekker et al, 1986) in a system including TOMAC, a cationic surfactant.…”

Protein separation by affinity extraction with reversed micelles of Span 85 modified with Cibacron Blue F3G-A

“…Similar influence was also reported in the recycling of other affinity-based reversed micelles [36]. In contrast, the loss of ionic surfactant in extraction processes was considered as the main reason for the decrease of protein extraction yield in the recycling of conventional ionic surfactant-based reversed micelles [37]. In the CB-Span 85 reversed micellar system, Span 85 molecules of low HLB value (1.8) have a high partition coefficient to the organic phase, so the amount of Span 85 lost in the aqueous phase was little in the extraction process.…”

Nondispersive membrane solvent back extraction of Phenol

“…While working with the organic phase on the tube side, if the organic phase pressure exceeded 1.72-3.45 kPa the organic phase would go into the micropores of the hydrophilic MHFs. Dekker et al (1987) have reported penetration of water into the micropores for large pore (0.1 pm) hydrophobic polypropylene fibers. This behavior most likely explains the tube-side experimental data for N , , for hydrophilic module 2 shown in Figure 1 1 .…”