Enzyme-free isothermal amplification strategy for the detection of tumor-associated biomarkers: A review

Wang, Wenjia; Ge, Qinyu; Zhao, Xueyan

doi:10.1016/j.trac.2023.116960

Cited by 21 publications

(11 citation statements)

References 133 publications

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“…They play a critical role in intercellular communication and immune response by transporting these molecules from the parent to the recipient cells . And tumor-derived sEVs with different biomarkers are crucial to cancer development and progression. , Hence, tumor-derived sEVs are considered to be important biomarkers for cancer diagnosis and prognosis. ,− However, the detection of sEVs in the early stages of cancer can be challenging due to their low abundance in bodily fluids. − …”

mentioning

confidence: 99%

Polymerase-Driven Logic Signal Amplification for the Detection of Small Extracellular Vesicle Surface Proteins and the Identification of Breast Cancer

Cheng

Luo

et al. 2023

Anal. Chem.

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Small extracellular vesicles (sEVs) derived from tumors contain a vast amount of cellular information and are regarded as a potential diagnostic biomarker for noninvasive cancer diagnosis. Nevertheless, it remains challenging to accurately measure sEVs from clinical samples due to the low abundance of these vesicles as well as their phenotypic heterogeneity. Herein, a polymerase-driven logic signal amplification system (PLSAS) was developed for the high-sensitivity detection of sEV surface proteins and breast cancer (BC) identification. Aptamers were introduced to serve as sensing modules to specifically recognize target proteins. By changing the input DNA sequences, two polymerase-driven primer exchange reaction systems were rationally designed for DNA logic computing. This allows for autonomous targeting of a limited number of targets using "OR" and "AND" logic, leading to a significant increase in fluorescence signals and enabling the specific and ultrasensitive detection of sEV surface proteins. In this work, we investigated surface proteins of mucin 1 (MUC1) and the epithelial cell adhesion molecule (EpCAM) as model proteins. When MUC1 or EpCAM proteins were used as single signal input in the "OR" DNA logic system, the detection limit of sEVs was 24 or 58 particles/μL, respectively. And MUC1 and EpCAM proteins of sEVs can be simultaneously detected in the AND logic method, which can significantly reduce the effect of phenotypic heterogeneity of sEVs to distinguish the source of sEVs derived from various mammary cell lines, such as MCF-7, MDA MB 231, SKBR3, and MCF-10A. The approach has achieved high discrimination in serologically tested positive BC samples (AUC 98.1%) and holds significant potential in advancing the early diagnosis and prognostic assessments of BC.

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mentioning

confidence: 99%

Polymerase-Driven Logic Signal Amplification for the Detection of Small Extracellular Vesicle Surface Proteins and the Identification of Breast Cancer

Cheng

Luo

et al. 2023

Anal. Chem.

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“…The lateral flow immunoassay strip-based detection [67][68][69][70][71][72] Advancements in molecular detection technologies have facilitated their integration with chipbased platforms, leading to the development of iPOCT devices. This groundbreaking innovation has enabled more precise and personalized clinical diagnostics and treatments, thereby significantly improving patient survival rates and quality of life.…”

Section: Rnamentioning

confidence: 99%

“…Molecular detection techniques have experienced rapid advancements, particularly in the field of clinical medicine. The application of techniques such as genetic testing, tumor biomarker detection, and pathogen detection has provided crucial evidence for early disease diagnosis and treatment 67–72 . Advancements in molecular detection technologies have facilitated their integration with chip‐based platforms, leading to the development of iPOCT devices.…”

Section: Molecular Detection Technologymentioning

confidence: 99%

Intelligent point of care testing for medicine diagnosis

Gao,

2024

Interdisciplinary Medicine

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Intelligent point‐of‐care testing (iPOCT) is a rapidly advancing technology that combines molecular detection and bio‐sensing techniques to achieve instant and accurate analysis through advanced sensors and intelligent algorithms. Molecular detection technologies, such as nucleic acid isothermal amplification, biochip and microfluidics, enable rapid analysis of the chemical composition and concentration of samples, thereby determining their presence. Bio‐sensing technology utilizes the recognition properties of biomolecules in conjunction with sensors to detect specific biomolecules or biological processes. iPOCT technology holds broad prospects for application in the field of healthcare and is expected to enhance detection sensitivity, accuracy, and intelligence through further development. In this review, we delineate the developmental journey of iPOCT, followed by a discussion on the principles of molecular detection and bio‐sensing technology, as well as the design of iPOCT devices for health monitoring and disease diagnosis. Furthermore, we showcased illustrative examples of iPOCT applications in disease diagnosis, highlighting the integration of iPOCT technology with wearable devices and smartphones for attaining more precise and personalized diagnostic results.

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“…After shaking at 25 °C for 12 h and centrifuging at 4000 rpm for 5 min, the supernatant was collected and analyzed by high-performance liquid chromatography (HPLC). The adsorption quantities of the HIR and HNIR were calculated using eqn (1), and the imprinting factors (IFs) were calculated using eqn (2).…”

Section: Adsorption Selectivitymentioning

confidence: 99%

“…Currently, the accurate detection of tumor-associated biomarkers enables early-stage diagnosis, monitoring, and therapy. 2 However, these biomarkers often exist in trace levels in the urine and biological samples of blood, which contain numerous interfering components, making their selective determination and separation challenging. [3][4][5] Therefore, the initial removal of interfering substances from real samples and biomarker enrichment is crucial for the accurate extraction and determination of biological samples.…”

Section: Introductionmentioning

confidence: 99%

Facile and green synthesis of a hydrophilic imprinted resin in a deep eutectic solvent–water medium for the specific molecular recognition of tumor biomarkers in complex biological matrices

Wang,

Zhou,

Qiao

et al. 2024

Green Chem.

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Enzyme-free isothermal amplification strategy for the detection of tumor-associated biomarkers: A review

Cited by 21 publications

References 133 publications

Polymerase-Driven Logic Signal Amplification for the Detection of Small Extracellular Vesicle Surface Proteins and the Identification of Breast Cancer

Polymerase-Driven Logic Signal Amplification for the Detection of Small Extracellular Vesicle Surface Proteins and the Identification of Breast Cancer

Intelligent point of care testing for medicine diagnosis

Facile and green synthesis of a hydrophilic imprinted resin in a deep eutectic solvent–water medium for the specific molecular recognition of tumor biomarkers in complex biological matrices

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