Enzyme- and Immuno-histochemical Studies on Root Resorption Activities Accompanied by the Physiological and Orthodontic Tooth Movement of Rat Molars.

Yagishita, Hisao; Sato, Kaori; Warita, Sachie; Ando, Fumihito; Aoba, Takaaki

doi:10.2330/joralbiosci1965.42.283

Cited by 6 publications

(6 citation statements)

References 12 publications

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“…These findings suggest that it may have been necessary to set a resting stage of orthodontic force for a certain fixed period of time between 14–21 days so that bone formation catches up with the amount of movement. In addition, it is the possible that occlusal trauma may have been produced because the occlusal condition varies with the increase in tooth movement (50).…”

Section: Discussionmentioning

confidence: 99%

Low‐energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling

Yoshida

Yamaguchi

Utsunomiya

et al. 2009

Orthod Craniofacial Res

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Section: Discussionmentioning

confidence: 99%

Low‐energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling

Yoshida

Yamaguchi

Utsunomiya

et al. 2009

Orthod Craniofacial Res

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“…In bone, it is very active in osteoblasts performing substrate repair, and strong activity is seen in osteoblasts actively engaged in substrate repair as well as in precalcified bone. 20,21 No ALP staining was detected in the nonirradiated group 3 days after odontectomy, but staining was seen along the rim of the alveolar bone at 7 days and 14 days. Staining in the bone margin was weaker in the irradiated group than in the nonirradiated group, and ALP activity in the alveolar socket declined after irradiation.…”

Section: Discussionmentioning

confidence: 84%

“…The results were generally in agreement with those previously reported by Iwata et al 9 TRAP is an enzyme used as a marker to identify osteoclasts. 21 Iwata et al 9 found that the number of TRAPpositive cells in the group exposed to a 10-Gy dose of X-rays tended to be greater soon after odontectomy than that in the nonirradiated group, although the difference was not significant. In the present study, the number of TRAPpositive cells 3 days after odontectomy was significantly greater in the irradiated group than that in the nonirradiated group, leading us to conclude that excessive bone resorption was taking place.…”

Section: Discussionmentioning

confidence: 95%

Experimental study on wound healing of alveolar bone sockets in the rat maxilla after X-ray irradiation

et al. 2002

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The purpose of this study was to examine the effects of radiation on the healing process of tooth extraction wounds. X-ray doses of 10 Gy were delivered once to the maxillofacial area of Wistar strain rats, and the maxillary first molar was extracted 7 days later. Animals were killed 3, 7, and 14 days after odontectomy, and thin sections of the maxilla were prepared. The specimens were stained with hematoxylin and eosin, azan, alkaline phosphatase and tartrate-resistant acid phosphatase (TRAP) stains for microscopic observation. The ratio of the area of the bone tissue to the area of the alveolar socket (BS/TS) and the numbers of TRAP-positive cells were evaluated. Seven days after odontectomy, BS/TS was significantly lower in the irradiated group than in the nonirradiated group. Alkaline phosphatase staining was observed along the rim of the bone 7 and 14 days after odontectomy in both groups, but it was milder in the irradiated group than in the nonirradiated group. TRAP-positive cells were present on the floor of the postextraction socket and the margins of the septal alveolar bone, and, 3 days after odontectomy, the number of TRAP-positive cells was significantly greater in the irradiation group than in the nonirradiation group.

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“…The specimens were dehydrated with ethanol and embedded in paraffin. Consecutive deparaffinized sections (4 m thick) were cut in the sagittal plane and stained with either hematoxylin/eosin or Azan, or processed for histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity using the azo dye method with 0.8% naphthol AS-BI phosphate as substrate (Yagishita et al 2000).…”

Section: Light Microscopymentioning

confidence: 99%

Immunodetection of Osteopontin at Sites of Resorption in the Pulp of Rat Molars

Shimazu

Nanci

Aoba

2002

J Histochem Cytochem.

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(YS,AN) S U M M A R Y Osteopontin (OPN) has been proposed to act as a substrate for osteoclast adhesion during bone resorption. The aim of the present study was to examine the presence and distribution of OPN at sites of resorption in traumatized radicular pulp. The upper first molars of 6-week-old male Sprague-Dawley rats were luxated and then repositioned in the original sockets. The animals were sacrificed by intracardiac perfusion at 10 and 14 days after tooth reimplantation. The teeth were decalcified in EDTA and then processed for embedding in paraffin for histochemistry or LR White resin for immunocytochemistry. Odontoclasts were identified by their multinucleated morphology and expression of tartrate-resistant acid phosphatase (TRAP). Osteopontin was immunolocalized using postembedding colloidal gold labeling with a chicken egg yolk anti-rat OPN antibody. After reimplantation of the teeth, TRAP-positive cells were present along the pulp dentin wall. Osteopontin was not consistently detected at exposed predentin/dentin surfaces. However, gold particles were often found at the margin of resorption lacunae. Labeling was also seen over the Golgi region and cytoplasmic vesicles of odontoclasts and of neutrophils and fibroblast-like cells. The results suggest that accumulation of OPN at the predentin/dentin surface is not a prerequisite for adhesion of odontoclasts to the wall substance and that recruited odontoclasts produce OPN locally to mediate cell and/or matrix events within the resorption lacuna.

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Enzyme- and Immuno-histochemical Studies on Root Resorption Activities Accompanied by the Physiological and Orthodontic Tooth Movement of Rat Molars.

Cited by 6 publications

References 12 publications

Low‐energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling

Low‐energy laser irradiation accelerates the velocity of tooth movement via stimulation of the alveolar bone remodeling

Experimental study on wound healing of alveolar bone sockets in the rat maxilla after X-ray irradiation

Immunodetection of Osteopontin at Sites of Resorption in the Pulp of Rat Molars

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