Enzymatic saccharification of acid–alkali pretreated sugarcane bagasse using commercial enzyme preparations

Abstract

BACKGROUND: The bioconversion of lignocellulosic biomass to second generation ethanol has the potential to replace some fossil fuels. Sugarcane bagasse (SB) is a readily available feedstock for ethanol production. The aim of this work was to study the enzymatic saccharification of consecutively acid-alkali pretreated SB using different commercial enzyme preparations. RESULTS:Under the most favourable enzymatic hydrolysis conditions, a maximum sugar recovery of 0.89 g of reducing sugars g -1 acid-alkali pretreated SB was obtained. Scanning electron microscopy (SEM) revealed the structural disruption in the SB morphology after acid-alkali pretreatment followed by enzymatic hydrolysis. CONCLUSION: These results improve research strategies and show the susceptibility of SB under different enzymatic actions.This is thought to be the first report on utilization of cellulase AF28918 for SB saccharification into fermentable sugars.

Integrated first‐ and second‐generation processes for bioethanol production from sugarcane

“…Capital costs may be high in equipment that must support high pressures or acid concentrations, such as in the pretreatment step, and in the large reactors required in hydrolysis due to low solids loading (usually up to 10%) and long reaction times (between 24 and 72 h). As mentioned previously, most of the hydrolysis reaction takes place within the first 24 h, and the incremental gains obtained from 24-48 h and 72 h hydrolysis times are much smaller (Banerjee et al 2012, Giese et al 2013. Since in integrated processes the unreacted material is ultimately burnt in the boilers, displacing additional lignocellulose for use as feedstock, there may be significant advantages to be gained from low hydrolysis reaction times for integrated processes.…”

“…Since unreacted solids are used as fuels in the cogeneration system, it may not be economical to use extremely long reaction times to achieve minor increases in yields (Giese et al 2013, Pereira et al 2011, Wu et al 2011). In addition, most hydrolysis studies are carried out using low solids loading (Giese et al 2013, Silva et al 2011, because hydrolysis yield and productivity are strongly affected by the solids content in hydrolysis reactors (Lau and Dale 2009). Despite leading to higher yields, the use of low solids loadings requires more water for dilution, generates more wastes, and requires more energy for concentration and purification, since it produces low ethanol titers.…”

Environmental assessment of residues generated after consecutive acid-base pretreatment of sugarcane bagasse by advanced oxidative process

“…In order to utilize carbohydrate fraction of SB for ethanol production via microbial fermentation, it is necessary to use appropriate pretreatment method for lignin removal. Recently, consecutive acid-base pretreatment process has been found successful for the efficient removal of hemicellulose and lignin leaving cellulose for the cellulolytic enzymes action for its conversion into glucose [6,7].…”

Effect of enzyme preparations on the main parameters of products in the development of processing technology of concentrated wort on ethanol

“…При разработке новых технологий производства этанола выбор режимов и технологических параметров получения осахаренного сусла базируется на аппаратурно-технологических и экономических аспектах. Немаловажную роль играют и свойства перерабатываемого сырья [2,3,[8][9][10].…”