1982
DOI: 10.1093/nar/10.12.3715
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Abstract: A combination of several enzymes, RNase-T1, nuclease S1, T4-polynucleotide kinase and T4-RNA ligase were used to prepare and modify different fragments of yeast tRNAAsp (normal anticodon G U C). This allowed us to reconstitute, in vitro, a chimeric tRNA that has any of the four bases G, A, U or C, as the first anticodon nucleotide, labelled with (32p) in its 3' position. Such reconstituted (32p) labelled yeast tRNAAsp were microinjected into the cytoplasm or the nucleus of the frog oocyte and checked for their… Show more

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Cited by 32 publications
(21 citation statements)
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“…Jean VACHER, Henri GROSJEAN, Suzanne DE HENAU, Jacqueline FINELLI, and Richard H. BUCKINGHAM Institut de Biologie Physico-Chimique, Paris; and Laboratoire de Chimie Biologique, Rhode St-Genese (Received July 8,1983) -EJB 83 0724…”
Section: Construction Of a Uga Suppressor Trna By Modification In Vitmentioning
confidence: 99%
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“…Jean VACHER, Henri GROSJEAN, Suzanne DE HENAU, Jacqueline FINELLI, and Richard H. BUCKINGHAM Institut de Biologie Physico-Chimique, Paris; and Laboratoire de Chimie Biologique, Rhode St-Genese (Received July 8,1983) -EJB 83 0724…”
Section: Construction Of a Uga Suppressor Trna By Modification In Vitmentioning
confidence: 99%
“…1) were isolated by partial digestion of tRNACyS. Preliminary experiments with different enzyme concentrations and times of incubation were performed to determine optimum conditions for digestion: 4 units Tl/mg tRNA, incubated for 90min at 4 "C; lo4 units S1 (Miles)/mg tRNA, incubated for 20 min at 37 "C. Other conditions were as described previously [8] except that S1 digestions included 10% glycerol. The TI fragment OH-C,,-A,,-OH and the S1 fragment pG1 -U,,-OH were purified on a polyacrylamide slab gel (13.5 % w/v acrylamide, 0.68 % bisacrylamide in 100 mM Tris/borate buffer, pH 8.3, 2.5 mM Na,EDTA and 8.3 M urea).…”
Section: Construction Of Trnacys (Anticodon Uca)mentioning
confidence: 99%
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