Enzymatic filter for improved separation of output signals in enzyme logic systems towards ‘sense and treat’ medicine

Kinetic Model for a Threshold Filter in an Enzymatic System for Bioanalytical and Biocomputing Applications

“…In this work we demonstrate that the added "filtering" mechanism in the referenced experiments 25 (and in the earlier work on filtering 81 …”

Diffusion of Oligonucleotides from within Iron‐Cross‐Linked, Polyelectrolyte‐Modified Alginate Beads: A Model System for Drug Release

“…Generally, alginate beads cross‐linked by metal ions can release loaded materials (here DNA) by several processes, which in many cases occur simultaneously . In addition to DNA diffusing out of the beads, the beads themselves can swell or break up in aqueous solutions, either on their own or by the destabilization of a protective surface layer . These processes are usually induced or significantly accelerated by adding chemicals that reduce the number of cross‐linking cations or recomplex them.…”

“…These composite structures offer favorable environments for embedding certain biomolecular species or/and additional gel stabilization. The added polymers were spread throughout the gel volume or, in some cases, they were used as external coatings deposited layer‐by‐layer on the gel surface . Depending on the specific composition of the alginate systems (e.g.…”

“…In some cases, the alginate gel (e.g. in the form of beads) can be fully decomposed and dissolved, which results in the release of all preloaded species. Under other circumstances, release of the embedded species could proceed without dissolution of the gel.…”

“…The major practical interest in many studies is signal‐triggered release, but leakage of the loaded species from intact alginate beads or thin films prior to application of the signal is also an important process. For practical realization of effective signal‐triggered release, uncontrolled leakage should be minimized . However, other studies, in which slow leakage was of interest and triggering signals were not used, focused on continuous slow delivery of the loaded species, for example, drugs .…”