Enzymatic degradation of poly(butylene succinate) copolyesters synthesized with the use of Candida antarctica lipase B

Abstract

<div><div><div><p>Abstract: Biodegradable polymers are an active area of investigation, particularly ones that can be produced from sustainable, biobased monomers, such as copolymers of poly(butylene succinate) (PBS). In this study, we examine the enzymatic degradation of poly(butylene succinate-dilinoleic succinate) (PBS-DLS) copolymers obtained by “green” enzymatic synthesis using lipase B from Candida antarctica (CALB). The copolymers differed in their hard to soft segments ratio, from 70:30 to 50:50 wt.%. Enzymatic degradation was carried out on electrospun membranes (scaffolds) and compression-moulded films using lipase fromPseudomomas cepacia. Poly(e-caprolactone) (PCL) was used as a reference aliphatic polyester. The degradation process was monitored gravimetrically via water uptake and mass loss. After 24 days, approx. 40% mass loss was observed for fibrous materials prepared from PBS-DLS 70:30 copolymer, as compared to approx. 10% mass loss for PBS-DLS 50:50. Infrared spectroscopy (FTIR) and SEC analysis were used to examine changes in chemical structure. Differential scanning calorimetry (DSC) and scanning light microscopy (LSM) revealed changes in degree of crystallinity, and changes in surface morphology, consistent with a surface erosion mechanism. We conclude that the obtained copolymers are suitable for tissue engineering applications thanks to tuneable degradation and lack of acidification during breakdown.</p></div></div></div>

Adsorption of Fibrinogen and Fibronectin on Elastomeric Poly(butylene Succinate) Copolyesters

“…In this regard, our interpretation is similar to and results are in the upper range of those reported previously for a number of different polymeric biomaterial thin films (Weber et al, 2007). Likewise, the viscosity values obtained for the Fg adlayers on all of the tested thin films were also broadly similar and also within the range of values previously reported (Weber et al, 2007). However, when looking at the shear modulus (Figure 4C), which can be interpreted to represent the stiffness of the adsorbed protein layer, the three PBS-DLS copolyesters demonstrated a trend towards lower shear modulus values than the two homopolymers.…”

“…Further, prior reports indicate that nanoscale surface roughness in the range of 2-8 nm-similar to values we determined using AFM (Table 4)-can enhance Fg adsorption, beyond that expected from the increase in surface area, by favoring an end-on orientation, due the anisotropy of the molecules (Rechendorff et al, 2006). In this regard, our interpretation is similar to and results are in the upper range of those reported previously for a number of different polymeric biomaterial thin films (Weber et al, 2007). Likewise, the viscosity values obtained for the Fg adlayers on all of the tested thin films were also broadly similar and also within the range of values previously reported (Weber et al, 2007).…”

“…PBS homopolymer and a series of elastomeric copolyesters with varying weight ratios on the feed (70:30, 60:40, 50:50) of hard segments consisting of butylene succinate (PBS) to dilinoleic succinate soft segments units (DLS) were synthesized using previously published procedure (Sonseca and El Fray, 2017;Wcisłek et al, 2018), described in the Supplemental Information. PBS homopolymer was also synthesized using the same procedure.…”

“…This family of copolymers, with varying hard to soft segment ratios, offers tunable mechanical properties typical of thermoplastic elastomers. More recently, our work with closely related PBS copolymers with DLS soft segments synthesized with enzymatic catalyst, has demonstrated the potential of these copolyesters for soft tissue engineering, as they are readily electrospinnable into 2D and 3D scaffolds and possess attractive mechanical and degradation properties (Sonseca and El Fray, 2017;Sonseca et al, 2018;Wcisłek et al, 2018). It is with these applications in mind, that we set out to study the adsorption of fibrinogen and fibronectin on PBS homopolymer and PBS-DLS copolymers with varying weight ratios (100:0, 70:30, 60:40, 50:50) of hard to soft segments.…”

Bio-Based Polymers with Antimicrobial Properties towards Sustainable Development

“…VOs are cheap, non-toxic, biodegradable, and, moreover, their polar character confers enhanced antibacterial properties to the resultant polymeric systems. Most utilized polyols from VOs are derived from soybean, sunflower, and cottonseed and, together with fatty acids, have been used for many years in the production of polyols employed in polyurethane and, to a lesser extent, in polyester synthesis [ 312 , 313 ].…”