Enzymatic characterization of Vibrio alginolyticus strains isolated from bivalves harvested at Venice Lagoon (Italy) and Guanabara Bay (Brazil)

Lafisca, Andrea; Pereira, Cláudio Márcio do Nascimento Abreu; Giaccone, Valério; Rodrigues, Dália dos Prazeres

doi:10.1590/s0036-46652008000400002

Cited by 29 publications

(19 citation statements)

References 8 publications

(14 reference statements)

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“…The mode of infection and transmission of this species remains to be studied, although it is probably seawater. The presence of V. alginolyticus may lead to wound infections, enteric diseases and sepsis in humans exposed to seawater (Lafisca et al, 2008;Masini et al, 2007). Moreover, V. alginolyticus is known to cause gastroenteritis (Darbas et al, 1992;Reina et al, 1995;Sabir et al, 2012;Traoré et al, 2012) in humans.…”

Section: Discussionmentioning

confidence: 99%

Hygiene and health risks associated with the consumption of edible lamellibranch molluscs

Carraro

Sanna

Brandas

et al. 2015

International Journal of Food Microbiology

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Section: Discussionmentioning

confidence: 99%

Hygiene and health risks associated with the consumption of edible lamellibranch molluscs

Carraro

Sanna

Brandas

et al. 2015

International Journal of Food Microbiology

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“…The Venice lagoon is an area of great economic importance for mollusk commerce. Little information is available concerning the distribution of Vibrionaceae in this area, and most of the studies are related to marine samples (e.g., sediments, plankton, or water) (52,53,54). In this work, we developed and applied an MLSA scheme based on four housekeeping genes, together with different statistical and predictive models, to characterize the Vibrio species from the Venice lagoon and to explore to what extent different ecologies represented distinct habitats for this genus.…”

Section: Discussionmentioning

confidence: 99%

Vibrio Trends in the Ecology of the Venice Lagoon

Rahman

Martino

Cardazzo

et al. 2014

Appl Environ Microbiol

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Vibrio is a very diverse genus that is responsible for different human and animal diseases. The accurate identification of Vibrio at the species level is important to assess the risks related to public health and diseases caused by aquatic organisms. The ecology of Vibrio spp., together with their genetic background, represents an important key for species discrimination and evolution. Thus, analyses of population structure and ecology association are necessary for reliable characterization of bacteria and to investigate whether bacterial species are going through adaptation processes. In this study, a population of Vibrionaceae was isolated from shellfish of the Venice lagoon and analyzed in depth to study its structure and distribution in the environment. A multilocus sequence analysis (MLSA) was developed on the basis of four housekeeping genes. Both molecular and biochemical approaches were used for species characterization, and the results were compared to assess the consistency of the two methods. In addition, strain ecology and the association between genetic information and environment were investigated through statistical models. The phylogenetic and population analyses achieved good species clustering, while biochemical identification was demonstrated to be imprecise. In addition, this study provided a fine-scale overview of the distribution of Vibrio spp. in the Venice lagoon, and the results highlighted a preferential association of the species toward specific ecological variables. These findings support the use of MLSA for taxonomic studies and demonstrate the need to consider environmental information to obtain broader and more accurate bacterial characterization. Vibrio spp. are Gram-negative halophilic bacteria belonging to the class Gammaproteobacteria. Vibrio is one of the most studied and diverse genera of microorganisms found in aquatic ecosystems and comprises the major culturable bacteria in marine and estuarine environments (1). According to the Association of Vibrio Biologists (AViB; http://www2.ioc.fiocruz.br/vibrio/AVib /species.html), there are 99 accepted or proposed Vibrio species, although the recent description of new species has led to a constantly changing taxonomy. Vibrio spp. are frequently isolated from fish, fish products, and edible shellfish, and a large number of species are pathogenic to different hosts. Some species, such as V. cholerae, V. parahaemolyticus, and V. vulnificus, cause serious food-borne gastroenteritis in humans. Other species, such as V. anguillarum and V. salmonicida, are pathogenic for fish; V. splendidus-related species are pathogenic for bivalves, and V. harveyi and V. campbellii are pathogenic for shrimps (1, 2, 3). Recently, Austin suggested a classification of zoonotic Vibrio in two classes named "higher-risk" vibrios (V. cholerae, V. parahaemolyticus, and V. vulnificus) and "lower-risk" vibrios (V. alginolyticus, V. fluvialis, V. furnissii, V. harveyi, and V. mimicus) (4). Bivalve mollusks such as clams and mussels represent products of great econ...

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“…; Lafisca et al . ; Austin ). Moreover, it has been also associated with mortalities in aquaculture (Darbas et al .…”

Section: Discussionmentioning

confidence: 99%

“…The clinical cases associated with V. alginolyticus are very rare and if any, the virulence factors triggering the illnesses are not clearly established for this species yet (Tantillo et al 2004;Schets et al 2006;Campanelli et al 2008). Vibrio alginolyticus is part of the natural microflora of marine organisms but it could constitute a threat as this species has been reported in diseased corals, fishes, crustaceans and bivalves molluscs (Balebona et al 1998;Kumar et al 2007;Wang et al 2007;Yan et al 2007;Cervino et al 2008;Harriague et al 2008;Lafisca et al 2008;Austin 2010). Moreover, it has been also associated with mortalities in aquaculture (Darbas et al 1992;Snoussi et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing

et al. 2013

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Aims: To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37°C on TCBS medium, in September 2009 from seawater and surface sediments. Methods and Results: q-PCR assays previously selected for the identification of bacterial strains isolated at 37°C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22°C. The great majority of the 37°C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85Á9%) and V. harveyi (10Á7%). The human pathogens V. parahaemolyticus and V. cholerae were rarely detected (two strains each). The 22°C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V. celticus (36Á8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22°C (80%) and 37°C (99Á5%), respectively. Conclusions: The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37°C both dominated by pathogenic species for marine organisms. Significance and Impact of the Study: The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22°C, as 92Á3% of them were identified in this study. IntroductionThe vibrios are widespread worldwide and ubiquitous in the aquatic environments, occupying various habitats including marine, freshwater and estuarine ecosystems as well as aquaculture facilities (Dalsgaard 1998;Sobecky et al. 1998;Urakawa et al. 1999;Heidelberg et al. 2002;Thompson et al. 2004b). To date, if we refer to the various banks of genomic sequences and to the on-line sources of Vibrio species inventory (Association of Vibrio Biologists, www.vibriobiology.net, last consultation 20 August 2012), the genus Vibrio includes 135 species . Numerous vibrios are pathogenic for marine organisms and 12 have been described as pathogenic for humans (Dalsgaard 1998;Paillard et al. 2004;Thompson et al. 2004a;Austin 2010). The sanitary and economic losses due to these pathogens, particularly to the species Vibrio cholerae, V. parahaemolyticus and

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Enzymatic characterization of Vibrio alginolyticus strains isolated from bivalves harvested at Venice Lagoon (Italy) and Guanabara Bay (Brazil)

Cited by 29 publications

References 8 publications

Hygiene and health risks associated with the consumption of edible lamellibranch molluscs

Hygiene and health risks associated with the consumption of edible lamellibranch molluscs

Vibrio Trends in the Ecology of the Venice Lagoon

Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing

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