volume 67, issue 1, P373-381 1993
DOI: 10.1128/jvi.67.1.373-381.1993
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Abstract: The poliovirus RNA-dependent RNA polymerase (3DP"°) shares a region of homology with all RNA polymerases, centered around the amino acid motif YGDD, which has been postulated to be involved in the catalytic activity of the enzyme. Using oligonucleotide site-directed mutagenesis, we substituted the tyrosine at this motif of the poliovirus RNA-dependent RNA polymerase with cysteine, histidine, isoleucine, methionine, phenylalanine, or serine. The enzymes were expressed in Escherichia coli, and in vitro enzyme ac…

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