Environmental enrichment attenuates the age-related decline in the mRNA expression of steroidogenic enzymes and reduces the methylation state of the steroid 5α-reductase type 1 gene in the rat hippocampus

Rossetti, María Florencia; Varayoud, Jorgelina; Moreno-Piovano, Guillermo S.; Luque, Enrique H.; Ramos, Jorge G.

doi:10.1016/j.mce.2015.05.024

Cited by 33 publications

(53 citation statements)

References 67 publications

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“…Such methylation constitutes an important mechanism of transcriptional regulation. Accordingly, changes in the methylation patterns of specific sites located in 5αR‐1 gene CpG island were correlated with alterations in the mRNA levels in the rat hippocampus .…”

Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 96%

“…Moreover, some forms of enrichment, including sensory and social stimuli, change the expression of 5αR‐1 in the brain. Specifically, rats housed in groups of eight animals in large cages and provided with an assortment of objects, including large plastic tubes, rodent dwellings and toys of various shapes, sizes and colors during 105 days, increased the transcription of 5αR‐1 in the hippocampus . Because this brain structure is associated with learning and memory, these results suggested that 5αR may play an important role in cognition.…”

Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 96%

“…Developmental changes in 3β‐HSD gene expression have been investigated in the brain of postnatal rodents. In the rat hippocampus, 3β‐HSD mRNA is two‐ to three‐fold higher on postnatal day (PND)7 and PND14 than PND70 ; and is nine‐fold higher on PND90 than on PND450 . In the cerebellum, 3β‐HSD is expressed transiently during PND7 and PND14 and disappears in the adult .…”

Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 99%

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Oestrogens and Progestagens: Synthesis and Action in the Brain

Rossetti

Cambiasso

Holschbach

et al. 2016

J Neuroendocrinology

107

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When steroids, such as pregnenolone, progesterone and oestrogen, are synthesised de novo in neural tissues, they are more specifically referred to as neurosteroids. These neurosteroids bind specific receptors to promote essential brain functions. Pregnenolone supports cognition and protects mouse hippocampal cells against glutamate and amyloid peptide-induced cell death. Progesterone promotes myelination, spinogenesis, synaptogenesis, neuronal survival and dendritic growth. Allopregnanolone increases hippocampal neurogenesis, neuronal survival and cognitive functions. Oestrogens, such as oestradiol, regulate synaptic plasticity, reproductive behaviour, aggressive behaviour and learning. In addition, neurosteroids are neuroprotective in animal models of Alzheimer's disease, Parkinson's disease, brain injury and ageing. Using in situ hybridisation and/or immunohistochemistry, steroidogenic enzymes, including cytochrome P450 side-chain cleavage, 3b-hydroxysteroid dehydrogenase/D5-D4 isomerase, cytochrome P450arom, steroid 5a-reductase and 3a-hydroxysteroid dehydrogenase, have been detected in numerous brain regions, including the hippocampus, hypothalamus and cerebral cortex. In the present review, we summarise some of the studies related to the synthesis and function of oestrogens and progestagens in the central nervous system.

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Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 96%

Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 96%

Section: Steroidogenic Enzymes In the Brainmentioning

confidence: 99%

See 1 more Smart Citation

Oestrogens and Progestagens: Synthesis and Action in the Brain

Rossetti

Cambiasso

Holschbach

et al. 2016

J Neuroendocrinology

107

View full text Add to dashboard Cite

show abstract

“…Epigenetic mechanisms associated with differential promoter methylation could be involved. Recent data from our group shows that lifestyle factors such as environmental enrichment affect mRNA expression and the DNA methylation state of steroidogenic enzymes in the hippocampus [41]. However, we did not study methylation state of ARO, 5R-1 or 3HSD in this experimental model and that is one of the limitations of the present study.…”

Section: Accepted Manuscriptmentioning

confidence: 72%

Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3-beta-HSD and ARO but lower 5-alpha-R-1 in male rats

et al. 2016

Self Cite

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Removing dietary phytoestrogens causes obesity and diabetes in adult male rats. Based on the facts that hypothalamic food intake control is disrupted in phytoestrogen-deprived animals and that several steroids affect food intake, we hypothesized that phytoestrogen withdrawal alters the expression of hypothalamic steroidogenic enzymes. Male Wistar rats fed with a high-phytoestrogen diet from conception to adulthood were subjected to phytoestrogen withdrawal by feeding them a low-phytoestrogen diet or a high-phytoestrogen, high-fat diet. Withdrawal of dietary phytoestrogens increased 3β-hydroxysteroid dehydrogenase and P450 aromatase gene expression and decreased those of 5α-reductase-1. This is a direct effect of the lack of dietary phytoestrogens and not a consequence of obesity, as it was not observed in high-fat-fed rats. Phytoestrogen withdrawal and high-fat diet intake reduced hypothalamic expression of estrogen receptor (ER)α correlated with low levels of ERα-O, ERα-OS, and ERα-OT transcripts. Variations in gene expression of steroidogenic enzymes may affect the content of neurosteroids. As neurosteroids are related to food intake control, the changes observed may be a novel mechanism in the regulation of energy balance in obese phytoestrogen-deprived animals. In rats, steroidogenesis and ER signaling appear to be altered by phytoestrogen withdrawal in the rat. The ubiquity of phytoestrogens in the diet and changing intakes or withdrawal suggest that aspects of human health could be affected based on the rat and warrant further research.

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“…The relevance of ALLO in memory processing, however, is strengthened by some observations. Indeed, it is known that ALLO increases neurogenesis and neuronal survival, reducing apoptosis in the hippocampus [35, 73]. ALLO also increases the density of dendritic spines, increasing the number of mature excitatory synapsis.…”

Section: Neurosteroids and Memorymentioning

confidence: 99%

Neurosteroids Involvement in the Epigenetic Control of Memory Formation and Storage

Colciago

Magnaghi

2016

Neural Plasticity

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Memory is our ability to store and remember past experiences; it is the result of changes in neuronal circuits of specific brain areas as the hippocampus. During memory formation, neurons integrate their functions and increase the strength of their connections, so that synaptic plasticity is improved and consolidated. All these processes recruit several proteins at the synapses, whose expression is highly regulated by DNA methylation and histone tails posttranslational modifications. Steroids are known to influence memory process, and, among them, neurosteroids are implicated in neurodegenerative disease related to memory loss and cognitive impairment. The epigenetic control of neurosteroids involvement in memory formation and maintenance could represent the basis for neuroregenerative therapies.

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Environmental enrichment attenuates the age-related decline in the mRNA expression of steroidogenic enzymes and reduces the methylation state of the steroid 5α-reductase type 1 gene in the rat hippocampus

Cited by 33 publications

References 67 publications

Oestrogens and Progestagens: Synthesis and Action in the Brain

Oestrogens and Progestagens: Synthesis and Action in the Brain

Dietary withdrawal of phytoestrogens resulted in higher gene expression of 3-beta-HSD and ARO but lower 5-alpha-R-1 in male rats

Neurosteroids Involvement in the Epigenetic Control of Memory Formation and Storage

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