Environmental cues influence EDC-mediated endocrine disruption effects in different developmental stages of Japanese medaka (Oryzias latipes)

Jin, Yuanxiang; Shu, Linjun; Huang, Feiyan; Cao, Limin; Sun, Liwei; Fu, Zhengwei

doi:10.1016/j.aquatox.2010.10.005

Cited by 26 publications

(14 citation statements)

References 41 publications

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“…This is particularly useful in light of the heightened sensitivity of juvenile fish to EDCs (Jin et al 2010;Peters et al 2009). Using silversides as a surrogate for examining the impact of endocrine disruption in North American estuaries is supported by of the high sensitivity of juveniles to ethinylestradiol (EE2) in this study.…”

Section: Resultsmentioning

confidence: 98%

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

2012

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A large body of work has established a link between endocrine disrupting compounds (EDCs) and a number of abnormalities in fishes. However, most EDC studies use several standard laboratory denizens to assess impacts, so assumptions about sensitivity are primarily based on these few species. Additionally, existing methods rely on obtaining sufficient plasma to measure EDC biomarkers. Our objectives were (a) to establish a new model species for estuarine fishes, (b) to evaluate endocrine impacts with a highly sensitive and specific biomarker, and (c) to develop a method for the analysis of this biomarker in small fish that do not possess sufficient blood plasma for protein measurement. As such, we created a polyclonal antibody (Ab) to the estrogen-responsive proteins chorion (Ch) and choriogenin (Chg) in Menidia beryllina, found throughout coastal North America and already utilized in EPA Whole Effluent Testing. We then validated the Ab by using it to measure the response to aqueous ethinylestradiol (EE2) through the development an ELISA using Menidia whole body homogenate (WBH). Sensitivity of the Ab to Menidia WBH is greater than that of the commercially available option. ELISA sensitivity, with a detection limit of 5 ng/ml and a working range of 22.6-1370.9 ng/ml, is comparable to ELISAs developed to measure plasma Chg. To our knowledge this is the first ELISA method developed for the detection of Chg using WBH. Including additional model species and methods allowing the evaluation of alternative sample matrices will contribute to an enhanced understanding of inter-species differences in EDC response.

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Section: Resultsmentioning

confidence: 98%

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

2012

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“…has been shown to be susceptible to estrogen exposure during the larval period [36]. Notably, our study is the first to look at protein-level endocrine disrupting effects of pyrethroids on juvenile fishes, which are more sensitive to such environmental EDC perturbations than adults [14] and more likely to experience long-term developmental changes such as reduced fecundity and/or intersex when exposed to EDCs [37]. …”

Section: Discussionmentioning

confidence: 99%

“…Pyrethroids also have considerable endocrine activity in vivo, particularly bifenthrin, permethrin and especially permethrin metabolites. These can induce estrogen-dependent egg proteins in male fish [13, 14]. Notably, permethrin metabolites induced relatively higher expression of such proteins than the parent compound in a recent study [13].…”

Section: Introductionmentioning

confidence: 99%

The in vivo estrogenic and in vitro anti‐estrogenic activity of permethrin and bifenthrin

Brander

Smalling

et al. 2012

Enviro Toxic and Chemistry

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Pyrethroids are highly toxic to fish at parts per billion or parts per trillion concentrations. Their intended mechanism is prolonged sodium channel opening, but recent studies reveal that pyrethroids such as permethrin and bifenthrin also have endocrine activity. Additionally, metabolites may have greater endocrine activity than parent compounds. We evaluated the in vivo concentration-dependent ability of bifenthrin and permethrin to induce choriogenin (an estrogen-responsive protein) in Menidia beryllina, a fish species known to reside in pyrethroid contaminated aquatic habitats. We then compared the in vivo response to an in vitro assay: CALUX (Chemical Activated Luciferase Gene Expression). Juvenile Menidia beryllina exposed to bifenthrin (1, 10, 100 ng/L), permethrin (0.1, 1, 10 µg/L), and ethinylestradiol (1, 10, 50 ng/L) had significantly higher ng/mL choriogenin (Chg) measured in whole body homogenate than controls. While Chg expression in fish exposed to ethinylestradiol (EE2) exhibited a traditional sigmoidal concentration-response, curves fit to Chg expressed in fish exposed to pyrethroids suggest a unimodal response, decreasing slightly as concentration increases. While the in vivo response indicated that bifenthrin and permethrin or their metabolites act as estrogen agonists, the CALUX assay demonstrated estrogen antagonism by the pyrethroids. Our results, supported by evidence from previous studies, suggest that bifenthrin and permethrin, and/or their metabolites, appear to act as estrogen receptor (ER) agonists in vivo, and that the unmetabolized pyrethroids, particularly bifenthrin, act as an ER antagonists in cultured mammalian cells.

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“…Unfortunately, little experimental work has been done to systematically explore this hypothesis. Studies have documented that temperature can affect the induction of vitellogenin (egg yolk protein) by exogenous estrogens in several different fish species 84–87; but other than this one pathway and endpoint, virtually nothing is known about chemical–temperature interactions on HPG function. To assess the scope of potential risk associated with these interactions on HPG functioning in fish, multiple HPG pathways and end points need to be assessed.…”

Section: Endocrine-disrupting Compounds and Gcc Interactionsmentioning

confidence: 99%

Interactions between chemical and climate stressors: A role for mechanistic toxicology in assessing climate change risks

Hooper

Ankley

Cristol

et al. 2012

Enviro Toxic and Chemistry

296

247

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Incorporation of global climate change (GCC) effects into assessments of chemical risk and injury requires integrated examinations of chemical and nonchemical stressors. Environmental variables altered by GCC (temperature, precipitation, salinity, pH) can influence the toxicokinetics of chemical absorption, distribution, metabolism, and excretion as well as toxicodynamic interactions between chemicals and target molecules. In addition, GCC challenges processes critical for coping with the external environment (water balance, thermoregulation, nutrition, and the immune, endocrine, and neurological systems), leaving organisms sensitive to even slight perturbations by chemicals when pushed to the limits of their physiological tolerance range. In simplest terms, GCC can make organisms more sensitive to chemical stressors, while alternatively, exposure to chemicals can make organisms more sensitive to GCC stressors. One challenge is to identify potential interactions between nonchemical and chemical stressors affecting key physiological processes in an organism. We employed adverse outcome pathways, constructs depicting linkages between mechanism-based molecular initiating events and impacts on individuals or populations, to assess how chemical- and climate-specific variables interact to lead to adverse outcomes. Case examples are presented for prospective scenarios, hypothesizing potential chemical–GCC interactions, and retrospective scenarios, proposing mechanisms for demonstrated chemical–climate interactions in natural populations. Understanding GCC interactions along adverse outcome pathways facilitates extrapolation between species or other levels of organization, development of hypotheses and focal areas for further research, and improved inputs for risk and resource injury assessments. Environ. Toxicol. Chem. 2013;32:32–48. © 2012 SETAC

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Environmental cues influence EDC-mediated endocrine disruption effects in different developmental stages of Japanese medaka (Oryzias latipes)

Cited by 26 publications

References 41 publications

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

An approach to detecting estrogenic endocrine disruption via choriogenin expression in an estuarine model fish species

The in vivo estrogenic and in vitro anti‐estrogenic activity of permethrin and bifenthrin

Interactions between chemical and climate stressors: A role for mechanistic toxicology in assessing climate change risks

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