Environmental and Genetic Factors Determine Whether the Mosquito Aedes aegypti Lays Eggs Without a Blood Meal

Ariani, Cristina V.; Smith, Sophia C. L.; Osei-Poku, Jewelna; Short, Katherine; Juneja, Punita; Jiggins, Francis M.

doi:10.4269/ajtmh.14-0471

Cited by 19 publications

(17 citation statements)

References 34 publications

(45 reference statements)

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“…13 Several factors which correlated to the choice of the place where the female mosquito puts its eggs are the presence of other specific larvae or pupae, sun exposure and the size of the container. 14 Result of this study in accordance with previous studies which found Aedes sp. laid their eggs more outdoors than indoors.…”

Section: Discussionsupporting

confidence: 92%

Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

2019

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Abstract. Gombong Subdistrict is one of dengue-endemic area in Kebumen Regency, Central Java. The high number of dengue cases in this area raises the question of whether there has been transovarial transmission occured from the Aedes sp. to their eggs. Transovarial transmission could be dangerous because the next generation of mosquitoes can directly become competent vectors as transmitters of the dengue virus (DENV). The purpose of this study is to detect dengue virus transovarial transmission in Aedes sp. in Gombong Subdistrict. This is a descriptive research in Gombong, Semanding, and Kali Tengah villages, Gombong Sub District. A total of 300 houses, 100 houses from each village were selected in this study. There were 600 Oviposition traps (ovitraps) were installed both inside and outside of houses for 6 days. Ovitraps were calculated by its Ovitrap Index (OI). Detection of transovarial transmission was carried by rearing field mosquitoes to Filial 1 then identified by RT-PCR assay. This study showed that OI in the three villages was higher in outdoor compared to indoor positions. All tested samples were negative DENV, indicated that there were no transovarial transmission occured at the study sites. Transmission in these study areas might still through horizontal mechanism transmission by mosquito bites. Although there is no transovarial transmission, awareness of dengue transmission must be continued by eradicating of mosquito nests such as 3M plus activities on a regular basis

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Section: Discussionsupporting

confidence: 92%

Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

2019

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“…While males are polygynous, they limit fertilization by other males both by transferring sufficient sperm to fertilize all eggs a female will lay in her lifetime, and by transmitting accessory gland secretions that act as physical and chemical barriers to insemination by other males [reviewed by Oliva et al (2014)]. After mating, egg maturation generally occurs after blood feeding, but can occur in its absence in a small portion (3-4%) of females (Ariani et al 2015). A. aegypti does not have heteromorphic sex chromosomes among its three pairs of chromosomes (Juneja et al 2014).…”

mentioning

confidence: 99%

Rapid Evolution of Ovarian-Biased Genes in the Yellow Fever Mosquito (Aedes aegypti)

Whittle

Extavour

2017

Genetics

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Males and females exhibit highly dimorphic phenotypes, particularly in their gonads, which is believed to be driven largely by differential gene expression. Typically, the protein sequences of genes upregulated in males, or male-biased genes, evolve rapidly as compared to female-biased and unbiased genes. To date, the specific study of gonad-biased genes remains uncommon in metazoans. Here, we identified and studied a total of 2927, 2013, and 4449 coding sequences (CDS) with ovary-biased, testis-biased, and unbiased expression, respectively, in the yellow fever mosquito The results showed that ovary-biased and unbiased CDS had higher nonsynonymous to synonymous substitution rates (dN/dS) and lower optimal codon usage (those codons that promote efficient translation) than testis-biased genes. Further, we observed higher dN/dS in ovary-biased genes than in testis-biased genes, even for genes coexpressed in nonsexual (embryo) tissues. Ovary-specific genes evolved exceptionally fast, as compared to testis- or embryo-specific genes, and exhibited higher frequency of positive selection. Genes with ovary expression were preferentially involved in olfactory binding and reception. We hypothesize that at least two potential mechanisms could explain rapid evolution of ovary-biased genes in this mosquito: (1) the evolutionary rate of ovary-biased genes may be accelerated by sexual selection (including female-female competition or male-mate choice) affecting olfactory genes during female swarming by males, and/or by adaptive evolution of olfactory signaling within the female reproductive system (, sperm-ovary signaling); and/or (2) testis-biased genes may exhibit decelerated evolutionary rates due to the formation of mating plugs in the female after copulation, which limits male-male sperm competition.

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“…It is also 520 possible that mosquitoes in search of an oviposition site may have never taken a blood 521 meal. While most mosquitoes usually require a blood meal to develop eggs, autogeny 522 has been reported in these species (Ariani et al, 2015;Chambers and Klowden, 1994;523 Olejnícek and Gelbic, 2000). Autogeny rates, which are variable and depend on 524 temperature and nutrition, have been reported to range from 3 -34% for Ae.…”

Section: Discussion 485mentioning

confidence: 99%

“…aegypti, 525 around 5% for Ae. albopictus, and up to 87% in Culex mosquitoes (Ariani et al, 2015;526 Chambers and Klowden, 1994;Olejnícek and Gelbic, 2000;Trpis, 1977). Little is known 527 regarding the influence of microbiota and autogeny, although in the autogenous 528 mosquito Ae.…”

Section: Discussion 485mentioning

confidence: 99%

Microbiome interaction networks and community structure from lab-reared and field-collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquito vectors

Hegde

Khanipov

Albayrak

et al. 2018

Preprint

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Abstract. 31Microbial interactions are an underappreciated force in shaping insect microbiome 32 communities. Although pairwise patterns of symbiont interactions have been identified, 33we have a poor understanding regarding the scale and the nature of co-occurrence and 34 co-exclusion interactions within the microbiome. To characterize these patterns in 35 mosquitoes, we sequenced the bacterial microbiome of Aedes aegypti, Ae. albopictus, 36and Culex quinquefasciatus caught in the field or reared in the laboratory and used 37 these data to generate interaction networks. For collections, we used traps that attracted 38 host-seeking or ovipositing female mosquitoes to determine how physiological state 39 affects the microbiome under field conditions. Interestingly, we saw few differences in 40 species richness or microbiome community structure in mosquitoes caught in either trap. 41Co-occurrence and co-exclusion analysis identified 116 pairwise interactions 42 substantially increasing the list of bacterial interactions observed in mosquitoes. 43Networks generated from the microbiome of Ae. aegypti often included highly 44 interconnected hub bacteria. There were several instances where co-occurring bacteria 45 co-excluded a third taxa, suggesting the existence of tripartite relationships. Several 46 associations were observed in multiple species or in field and laboratory-reared 47 mosquitoes indicating these associations are robust and not influenced by 48 environmental or host factors. To demonstrate that microbial interactions can influence 49 colonization of the host, we administered symbionts to Ae. aegypti larvae that either 50 possessed or lacked their resident microbiota. We found that the presence of resident 51 . CC-BY-NC-ND 4.0 International license It is made available under a was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint (which . http://dx.doi.org/10.1101/337311 doi: bioRxiv preprint first posted online Jun. 4, 2018; 4 microbiota can inhibit colonization of particular bacterial taxa. Our results highlight that 52 microbial interactions in mosquitoes are complex and influence microbiome composition. 53 54

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Environmental and Genetic Factors Determine Whether the Mosquito Aedes aegypti Lays Eggs Without a Blood Meal

Cited by 19 publications

References 34 publications

Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

Rapid Evolution of Ovarian-Biased Genes in the Yellow Fever Mosquito (Aedes aegypti)

Microbiome interaction networks and community structure from lab-reared and field-collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquito vectors

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