Envelope-Stress Sensing Mechanism of Rcs and Cpx Signaling Pathways in Gram-Negative Bacteria

Cho, Seung-Hyun; Dekoninck, Kilian; Collet, Jean-François

doi:10.1007/s12275-023-00030-y

Cited by 10 publications

(6 citation statements)

References 106 publications

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“…Normal activation of The Cpx system boosts chaperone and protease expression, along with enhancing peptidoglycan modifications by proteins, all contributing to the response of cell to envelope stress (Mitchell and Silhavy, 2019 ). In the process of activation and response, two of the famous proteins are NlpE, an OM lipoprotein that acts as a signaling module activator (Cho et al, 2023 ), and DegP, a periplasmic protease triggered to break down misfolded proteins upon CpxA/CpxR system activation. However, this study observed that the gene expression levels of nlpE (VM_05160) and degP (VM_12340) were downregulated, although not significantly.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01

Qin,

Peng,

Feng

et al. 2024

Front. Microbiol.

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HtpG, a bacterial homolog of the eukaryotic 90 kDa heat-shock protein (Hsp90), represents the simplest member of the heat shock protein family. While the significance of Hsp90 in fungal and cancer drug resistance has been confirmed, the role of HtpG in bacterial antibiotic resistance remains largely unexplored. This research aims to investigate the impact of the htpG gene on antibiotic resistance in Vibrio mimicus. Through the creation of htpG gene deletion and complementation strains, we have uncovered the essential role of htpG in regulating the structural integrity of the bacterial cell envelope. Our transcriptomics analysis demonstrates that the deletion of htpG increases the sensitivity of V. mimicus to antimicrobial peptides, primarily due to upregulated lipopolysaccharide synthesis, reduced glycerophospholipid content, and weakened efflux pumps activity. Conversely, reduced sensitivity to β-lactam antibiotics in the ΔhtpG strain results from decreased peptidoglycan synthesis and dysregulated peptidoglycan recycling and regulation. Further exploration of specific pathway components is essential for a comprehensive understanding of htpG-mediated resistance mechanisms, aiding in the development of antimicrobial agents. To our knowledge, this is the first effort to explore the relationship between htpG and drug resistance in bacteria.

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Section: Discussionmentioning

confidence: 99%

Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01

Qin,

Peng,

Feng

et al. 2024

Front. Microbiol.

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“…Y. enterocolitica TCS1 is identified as CpxAR. CpXAR is associated with membrane stress response in gram negative bacteria (Cho et al, 2023). Together, these results demonstrate the importance of internal replicates afforded by barcoding to further identify robust sensors.…”

Section: Resultsmentioning

confidence: 99%

A discovery platform to identify inducible synthetic circuitry from varied microbial sources

Robinson,

Carreno,

Weber

et al. 2023

Preprint

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Gut-resident microbes encode a variety of systems capable of sophisticated sensing and conditional gene expression control within the mammalian gut during health and disease. Bacterial synthetic biology approaches, such as whole-cell biosensing and 'sense-and-respond' therapeutics, aim to re-purpose such systems for research and clinical applications; however, the toolbox of well characterised in vivo and disease-related inducible systems remains limited. Here, we dramatically expand the flexibility and power of a high-throughput approach to biosensor evaluation using bacterial memory circuits encoded in a gut commensal E. coli species. We construct a novel library of bacterial two-component system (TCS) based biosensors sourced from gut pathogens, tagged with unique DNA barcodes using a single-pot construction method. Using our pipeline, we evaluate sensor activity and performance heterogeneity across in vitro and in vivo conditions leading to novel sensor discovery. In the process, we optimise library construction, sample quality thresholds, and parameters for engineered bacterial biosensor screening in the murine gut. This approach can be applied to transcriptionally activated sensing elements of any type and will allow rapid development of new biosensors that can advance synthetic biology approaches for complex environments, such as the gut.

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“…Due to their additional outer membrane, which serves as a permeability barrier, Gram-negative bacteria have demonstrated greater resistance to antibacterial agents compared to Gram-positive bacteria [ 55 , 56 ]. The ESR in E. coli is complex and involves multiple stress-sensing mechanisms, such as the σE response, the Cpx response, and the rcsBCDF phosphorelay system, to monitor the cell envelope for damage and to restore homeostasis [ 47 ].…”

Section: Resultsmentioning

confidence: 99%

Physiological and Transcriptomic Analyses of Escherichia coli Serotype O157:H7 in Response to Rhamnolipid Treatment

Yang,

Ma,

et al. 2023

Microorganisms

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Rhamnolipid (RL) can inhibit biofilm formation of Escherichia coli O157:H7, but the associated mechanism remains unknown. We here conducted comparative physiological and transcriptomic analyses of cultures treated with RL and untreated cultures to elucidate a potential mechanism by which RL may inhibit biofilm formation in E. coli O157:H7. Anti-biofilm assays showed that over 70% of the E. coli O157:H7 biofilm formation capacity was inhibited by treatment with 0.25–1 mg/mL of RL. Cellular-level physiological analysis revealed that a high concentration of RL significantly reduced outer membrane hydrophobicity. E. coli cell membrane integrity and permeability were also significantly affected by RL due to an increase in the release of lipopolysaccharide (LPS) from the cell membrane. Furthermore, transcriptomic profiling showed 2601 differentially expressed genes (1344 up-regulated and 1257 down-regulated) in cells treated with RL compared to untreated cells. Functional enrichment analysis indicated that RL treatment up-regulated biosynthetic genes responsible for LPS synthesis, outer membrane protein synthesis, and flagellar assembly, and down-regulated genes required for poly-N-acetyl-glucosamine biosynthesis and genes present in the locus of enterocyte effacement pathogenicity island. In summary, RL treatment inhibited E. coli O157:H7 biofilm formation by modifying key outer membrane surface properties and expression levels of adhesion genes.

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Envelope-Stress Sensing Mechanism of Rcs and Cpx Signaling Pathways in Gram-Negative Bacteria

Cited by 10 publications

References 106 publications

Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01

Transcriptome reveals the role of the htpG gene in mediating antibiotic resistance through cell envelope modulation in Vibrio mimicus SCCF01

A discovery platform to identify inducible synthetic circuitry from varied microbial sources

Physiological and Transcriptomic Analyses of Escherichia coli Serotype O157:H7 in Response to Rhamnolipid Treatment

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