Enumeration and viability of rare cells in a microfluidic disk via positive selection approach

Chen, Ken-Chao; Pan, Yu-Cheng; Chen, Chen-Lin; Lin, Ching-Hung; Huang, Chiun‐Sheng; Wo, Andrew M.

doi:10.1016/j.ab.2012.07.007

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…The magnetically labeled cells can be either deflected or immobilized using the interplay of centrifugal and magnetic forces, and can thus be separated from the non-labeled cells. In positive selection approaches, rare MCF-7 cancer cells have been separated from background Jurkat cells 217 or whole blood 218 using on-disk magnets. In a negative selection approach, non-target cells labeled with magnetic beads were separated from target MCF-7 cells with rarities down to 10 −6 .…”

Section: Applicationsmentioning

confidence: 99%

Centrifugal microfluidic platforms: advanced unit operations and applications

et al. 2015

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Centrifugal microfluidics has evolved into a mature technology. Several major diagnostic companies either have products on the market or are currently evaluating centrifugal microfluidics for product development. The fields of application are widespread and include clinical chemistry, immunodiagnostics and protein analysis, cell handling, molecular diagnostics, as well as food, water, and soil analysis. Nevertheless, new fluidic functions and applications that expand the possibilities of centrifugal microfluidics are being introduced at a high pace. In this review, we first present an up-to-date comprehensive overview of centrifugal microfluidic unit operations. Then, we introduce the term "process chain" to review how these unit operations can be combined for the automation of laboratory workflows. Such aggregation of basic functionalities enables efficient fluidic design at a higher level of integration. Furthermore, we analyze how novel, ground-breaking unit operations may foster the integration of more complex applications. Among these are the storage of pneumatic energy to realize complex switching sequences or to pump liquids radially inward, as well as the complete pre-storage and release of reagents. In this context, centrifugal microfluidics provides major advantages over other microfluidic actuation principles: the pulse-free inertial liquid propulsion provided by centrifugal microfluidics allows for closed fluidic systems that are free of any interfaces to external pumps. Processed volumes are easily scalable from nanoliters to milliliters. Volume forces can be adjusted by rotation and thus, even for very small volumes, surface forces may easily be overcome in the centrifugal gravity field which enables the efficient separation of nanoliter volumes from channels, chambers or sensor matrixes as well as the removal of any disturbing bubbles. In summary, centrifugal microfluidics takes advantage of a comprehensive set of fluidic unit operations such as liquid transport, metering, mixing and valving. The available unit operations cover the entire range of automated liquid handling requirements and enable efficient miniaturization, parallelization, and integration of assays.

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Section: Applicationsmentioning

confidence: 99%

Centrifugal microfluidic platforms: advanced unit operations and applications

et al. 2015

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“…For instance, Chen CL et al, and Chen KC et al used a disk-shaped carrier board to fabricate a microchannel device into which biomarkers, magnetic particles, and antibodies conjugated with an imaging dye could be automatically and sequentially injected for analysis. [48][49][50] In addition to such disk magnets, pillar-shaped magnets and microfluidic devices that use micropumps are also being used to transport, wash, and detect desired biomarkers (ie, DNA, C-reactive protein). 51,52 The general direction of such automated devices is towards the assembly of a portable device.…”

Section: Automationmentioning

confidence: 99%

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Park¹,

Hwang²,

Lee³

2013

IJN

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The emergence of biomarkers as key players in the paradigm shift towards preventative medicine underscores the need for their detection and quantification. Advances made in the field of nanotechnology have played a crucial role in achieving these needs, and have contributed to recent advances in the field of medicine. Nanoparticle-based immunomagnetic assays, in particular, offer numerous advantages that utilize the unique physical properties of magnetic nanoparticles. In this review, we focus on recent developments and trends with regards to immunomagnetic assays used for detection of biomarkers. The various immunomagnetic assays are categorized into the following: particle-based multiplexing, signal control, microfluidics, microarray, and automation. Herein, we analyze each category and discuss their advantages and disadvantages.

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“…20 Density gradient approaches have been used alone or in combination with other methods. 22,23 The magnetic approach, often integrated with antibodies, has been wildly used as a positive selection methodology 24,25 toward target rare cells with known expression, or negative selection, 26,27 to remove unwanted cells, e.g., leukocytes. Fetal cells or trophoblasts from the circulation have been a much sought-after target, since a single cell holds promise for the entire fetal genome.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Another example of the physical approach coupled with inertial microfluidics with deterministic lateral displacement showed excellent analytical separation of 15 and 7μm particles . Density gradient approaches have been used alone or in combination with other methods. , The magnetic approach, often integrated with antibodies, has been wildly used as a positive selection methodology , toward target rare cells with known expression, or negative selection, , to remove unwanted cells, e.g., leukocytes.…”

Section: Introductionmentioning

confidence: 99%

A Systemic Approach to Isolate, Retrieve, and Characterize Trophoblasts from the Maternal Circulation Using a Centrifugal Microfluidic Disc and a Multiple Single-Cell Retrieval Strategy

Kang

Hsu

et al. 2023

Anal. Chem.

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Rare cells in the blood often have rich clinical significance. Although their isolation is highly desirable, this goal remains elusive due to their rarity. This paper presents a systemic approach to isolate and characterize trophoblasts from the maternal circulation. A microfluidic rare cell disc assay (RaCDA) was designed to process an extremely large volume of up to 15 mL of blood in 30 min, depleting red blood cells (RBCs) and RBC-bound white blood cells (WBC) while isolating trophoblasts in the collection chip. To minimize cell loss, on-disc labeling of cells with fluorescent immuno-staining identified the trophoblasts. Retrieval of trophoblasts utilized an optimized strategy in which multiple single cells were retrieved within the same micropipette column, with each cell encapsulated in a fluid volume (50 nL) separated by an air pocket (10 nL). Further, whole-genome amplification (WGA) amplified contents from a few retrieved cells, followed by quality control (QC) on the success of WGA via housekeeping genes. For definitive confirmation of trophoblasts, short-tandem repeat (STR) of the WGAamplified content was compared against STR from maternal WBC and amniocytes from amniocentesis. Results showed a mean recovery rate (capture efficiency) of 91.0% for spiked cells with a WBC depletion rate of 99.91%. The retrieval efficiency of single target cells of 100% was achieved for up to four single cells retrieved per micropipette column. Comparison of STR signatures revealed that the RaCDA can retrieve trophoblasts from the maternal circulation.

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Enumeration and viability of rare cells in a microfluidic disk via positive selection approach

Cited by 10 publications

References 22 publications

Centrifugal microfluidic platforms: advanced unit operations and applications

Centrifugal microfluidic platforms: advanced unit operations and applications

Immunomagnetic nanoparticle-based assays for detection of biomarkers

A Systemic Approach to Isolate, Retrieve, and Characterize Trophoblasts from the Maternal Circulation Using a Centrifugal Microfluidic Disc and a Multiple Single-Cell Retrieval Strategy

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