Enterococcus faecium Regulates Honey Bee Developmental Genes

Du, Yan; Luo, Shiqi; Zhou, Xin

doi:10.3390/ijms222212105

Cited by 9 publications

(4 citation statements)

References 90 publications

(104 reference statements)

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“…have also been found to be probiotic and beneficial in several biological models, including in protecting honey bees from American foulbrood disease and improving fish health, so we hypothesize that the gut microbiota might be an important factor driving differences in resistance to N. bombycis infection. 56,57 Consistent with the KEGG prediction, the changes in gut bacteria caused by microspores may contribute to intestinal dysfunction.…”

Section: Discussionmentioning

confidence: 61%

The gut commensal bacterium Enterococcus faecalis LX10 contributes to defending against Nosema bombycis infection in Bombyx mori

Zhang

Feng

et al. 2022

Pest Management Science

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BACKGROUND: Microsporidia, a group of obligate intracellular fungal-related parasites, have been used as efficient biocontrol agents for agriculture and forestry pests due to their host specificity and transovarial transmission. They mainly infect insect pests through the intestinal tract, but the interactions between microsporidia and the gut microbiota of the host have not been well demonstrated.RESULTS: Based on the microsporidia-Bombyx mori model, we report that the susceptibility of silkworms to exposure to the microsporidium Nosema bombycis was both dose and time dependent. Comparative analyses of the silkworm gut microbiome revealed substantially increased abundance of Enterococcus belonging to Firmicutes after N. bombycis infection. Furthermore, a bacterial strain (LX10) was obtained from the gut of B. mori and identified as Enterococcus faecalis based on 16S rRNA sequence analysis. E. faecalis LX10 reduced the N. bombycis spore germination rate and the infection efficiency in vitro and in vivo, as confirmed by bioassay tests and histopathological analyses. In addition, after simultaneous oral feeding with E. faecalis LX10 and N. bombycis, gene (Akirin, Cecropin A, Mesh, Ssk, DUOX and NOS) expression, hydrogen peroxide and nitric oxide levels, and glutathione S-transferase (GST) activity showed different degrees of recovery and correction compared with those under N. bombycis infection alone. Finally, the enterococcin LX protein was identified from sterile LX10 fermentation liquid based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis.CONCLUSION: Altogether, the results revealed that E. faecalis LX10 with anti-N. bombycis activity might play an important role in protecting silkworms from microsporidia. Removal of these specific commensal bacteria with antibiotics and utilization of transgenic symbiotic systems may effectively improve the biocontrol value of microsporidia.

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Section: Discussionmentioning

confidence: 61%

The gut commensal bacterium Enterococcus faecalis LX10 contributes to defending against Nosema bombycis infection in Bombyx mori

Zhang

Feng

et al. 2022

Pest Management Science

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“…Members of the Drosophila intestinal ora can affect the genetic factors of some animals and the composition of important nutrients in the body, such as triglyceride (fat) content; high diversity among intestinal microorganisms can improve the digestion and absorption of nutrients and increase nutritional reserves in the host body 36 . Many animals have an intestinal region with a low pH to aid protein digestion, absorb nutrients such as calcium, iron and vitamin B 12 , and kill enteric pathogens and parasites acquired orally 37 . Our current study showed that E. faecium can utilise carbohydrates (glucose) and lower the pH to 5.1 (Fig.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Enterococcus faecium and determination of its mechanism for promoting the growth and development of Drosophila

Pan

Gao

et al. 2022

Preprint

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Intestinal symbiotic microorganisms have a strong capacity to regulate the physiological functions of their host, and Drosophila serves as a useful model. Enterococcus faecium (E. faecium) is a member of the normal intestinal flora of animals. Lactic acid bacteria (LAB) such as E. faecium can promote the growth and development of Drosophila, but the mechanism of regulation of Drosophila is poorly understood. In this study, we found that E. faecium used a carbon source to produce probiotic acids. E. faecium is a symbiotic bacterium for Drosophila, and adult flies passed on parental flora to offspring. E. faecium promoted the growth and development of Drosophila, especially under poor nutritional conditions. E. faecium shortened the developmental process for Drosophila and accelerated the transformation from larva to pupa. Finally, E. faecium promoted the growth and development of Drosophila through TOR and insulin signalling pathways.

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“…As demonstrated in Drosophila (Daisley et al ., 2017), this immune stimulation may help honeybees to defend against pathogen susceptibility caused by pesticides exposure. The lactic acid bacterium, Enterococcus faecium , can reduce microsporidian parasite N. ceranae proliferation, increase bee survivorship after infection, and promote honeybee growth (Borges et al ., 2021; Du et al ., 2021). The probiotics for honeybees have been discussed in detail (Nowak et al ., 2021).…”

Section: Potential Roles Of the Gut Microbiota In Pathogen Resistancementioning

confidence: 99%

Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

Zhang

Zheng

2022

Insect Science

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Eusocial bumble and honey bees are important pollinators for global ecology and the agricultural economy. Although both the bumble and honey bees possess similar and host‐restricted gut microbiota, they differ in aspects of morphology, autonomy, physiology, behavior, and life cycle. The social bee gut bacteria exhibit host specificity that is likely a result of long‐term co‐evolution. The unique life cycle of bumblebees is key for the acquisition and development of their gut microbiota, and affects the strain‐level diversity of the core bacterial species. Studies on bumblebee gut bacteria show that they retain less functional capacity for carbohydrate metabolism compared with that of the honeybee. We discuss the potential roles of the bumblebee gut microbiota against pathogenic threats and the application of host‐specific probiotics for bumblebees. Given the advantages of the bumblebee microbiome, including the simple structure and host specificity, and the ease of manipulating bumblebee colonies, we propose that bumblebees may provide a valuable system for understanding the general principles of host–microbe interactions, gut–brain axis, and vertical transmission.

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Enterococcus faecium Regulates Honey Bee Developmental Genes

Cited by 9 publications

References 90 publications

The gut commensal bacterium Enterococcus faecalis LX10 contributes to defending against Nosema bombycis infection in Bombyx mori

The gut commensal bacterium Enterococcus faecalis LX10 contributes to defending against Nosema bombycis infection in Bombyx mori

Isolation of Enterococcus faecium and determination of its mechanism for promoting the growth and development of Drosophila

Bumblebees with the socially transmitted microbiome: A novel model organism for gut microbiota research

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