Enteroaggregative Escherichia coli (EAEC) strains enter and survive within cultured intestinal epithelial cells

Pereira, Ana Claudia Machado; Britto-Filho, Jonas D.; Carvalho, Jorge José de; Luna, Maria das Graças de; Rosa, Ana Cláudia P.

doi:10.1016/j.micpath.2008.07.001

Cited by 25 publications

(18 citation statements)

References 12 publications

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“…Therefore, the ability of the EAEC strains to invade T84 cell monolayers was quantified using a standard antibiotic protection assay. Most strains showed internalisation rates compatible with invasive strains [29]. However, levels of invasion varied greatly between EAEC strains from a low of 0.03% to a high of 20% (Fig.…”

Section: Resultsmentioning

confidence: 93%

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Genotypic and Phenotypic Characterisation of Enteroaggregative Escherichia coli from Children in Rio de Janeiro, Brazil

et al. 2013

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Enteroaggregative Escherichia coli (EAEC) is a significant cause of diarrhoeal illness in both children and adults. Genetic heterogeneity and recovery of EAEC strains from both healthy and diseased individuals complicates our understanding of EAEC pathogenesis. We wished to establish if genetic or phenotypic attributes could be used to distinguish between strains asymptomatically colonising healthy individuals and those which cause disease. Genotypic screening of a collection of twenty four EAEC isolates from children with and without diarrhoea revealed no significant differences in the repertoire of putative virulence factors present in either group of strains. In contrast, EAEC strains from phylogroup A were more strongly associated with asymptomatic groups whereas strains from phylogroup D were more associated with cases of diarrhoea. Phenotypic screening revealed no differences in the ability of strains from either cohort of children to form biofilms, to adhere to and invade cells in tissue culture or to cause disease in the Caenorhabditis elegans model of infection. However, the latter assay did reveal significant reduction in nematode killing rates when specific virulence factors were deleted from human pathogenic strains. Our results suggest that current models of infection are not useful for distinguishing avirulent from pathogenic strains of EAEC but can be useful in studying the effect of specific virulence factors.

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Section: Resultsmentioning

confidence: 93%

“…Previous investigations revealed some EAEC strains possessed the ability to invade cells [29]. Therefore, the ability of the EAEC strains to invade T84 cell monolayers was quantified using a standard antibiotic protection assay.…”

Section: Resultsmentioning

confidence: 99%

Genotypic and Phenotypic Characterisation of Enteroaggregative Escherichia coli from Children in Rio de Janeiro, Brazil

et al. 2013

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“…In adult patients in the United States, EAEC is susceptible to rifaximin or a single dose of azithromycin with or without loperamide (670,674,675). Elimination of persistent diarrhea may be problematic, as internalized EAEC was observed in intestinal epithelial cells in vitro, suggesting that EAEC may protect against host clearance by intracellular persistence (676).…”

Section: Clinical Considerationsmentioning

confidence: 99%

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

et al. 2013

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SUMMARY Although Escherichia coli can be an innocuous resident of the gastrointestinal tract, it also has the pathogenic capacity to cause significant diarrheal and extraintestinal diseases. Pathogenic variants of E. coli (pathovars or pathotypes) cause much morbidity and mortality worldwide. Consequently, pathogenic E. coli is widely studied in humans, animals, food, and the environment. While there are many common features that these pathotypes employ to colonize the intestinal mucosa and cause disease, the course, onset, and complications vary significantly. Outbreaks are common in developed and developing countries, and they sometimes have fatal consequences. Many of these pathotypes are a major public health concern as they have low infectious doses and are transmitted through ubiquitous mediums, including food and water. The seriousness of pathogenic E. coli is exemplified by dedicated national and international surveillance programs that monitor and track outbreaks; unfortunately, this surveillance is often lacking in developing countries. While not all pathotypes carry the same public health profile, they all carry an enormous potential to cause disease and continue to present challenges to human health. This comprehensive review highlights recent advances in our understanding of the intestinal pathotypes of E. coli .

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“…2). Knowledge of the stages of EAEC pathogenicity has been obtained from studies of in vitro cell cultures, animal models, and patients infected with EAEC (10,(13)(14)(15)(16)(17).…”

Section: Pathogenicity Of Eaecmentioning

confidence: 99%

Epidemiology and Clinical Manifestations of Enteroaggregative Escherichia coli

et al. 2014

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SUMMARY Enteroaggregative Escherichia coli (EAEC) represents a heterogeneous group of E. coli strains. The pathogenicity and clinical relevance of these bacteria are still controversial. In this review, we describe the clinical significance of EAEC regarding patterns of infection in humans, transmission, reservoirs, and symptoms. Manifestations associated with EAEC infection include watery diarrhea, mucoid diarrhea, low-grade fever, nausea, tenesmus, and borborygmi. In early studies, EAEC was considered to be an opportunistic pathogen associated with diarrhea in HIV patients and in malnourished children in developing countries. In recent studies, associations with traveler's diarrhea, the occurrence of diarrhea cases in industrialized countries, and outbreaks of diarrhea in Europe and Asia have been reported. In the spring of 2011, a large outbreak of hemolytic-uremic syndrome (HUS) and hemorrhagic colitis occurred in Germany due to an EAEC O104:H4 strain, causing 54 deaths and 855 cases of HUS. This strain produces the potent Shiga toxin along with the aggregative fimbriae. An outbreak of urinary tract infection associated with EAEC in Copenhagen, Denmark, occurred in 1991; this involved extensive production of biofilm, an important characteristic of the pathogenicity of EAEC. However, the heterogeneity of EAEC continues to complicate diagnostics and also our understanding of pathogenicity.

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Enteroaggregative Escherichia coli (EAEC) strains enter and survive within cultured intestinal epithelial cells

Cited by 25 publications

References 12 publications

Genotypic and Phenotypic Characterisation of Enteroaggregative Escherichia coli from Children in Rio de Janeiro, Brazil

Genotypic and Phenotypic Characterisation of Enteroaggregative Escherichia coli from Children in Rio de Janeiro, Brazil

Recent Advances in Understanding Enteric Pathogenic Escherichia coli

Epidemiology and Clinical Manifestations of Enteroaggregative Escherichia coli

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