Enteric, hepatic and muscle tissue development of goat kids fed with lyophilized bovine colostrum

Abstract

The objective of this study was to investigate the development of the enteric, hepatic and muscle tissues in goat kids fed with lyophilized bovine colostrum in the transition period of passive immunity to early active immunity. At 0, 7 and 14 h of life, 15 male newborns received 5% of their body weight of lyophilized bovine colostrum and 14 male newborns received goat colostrum, both with 55 mg/ml of IgG. Samples of the duodenum, jejunum, ileum, liver and muscle were collected at 18, 36 and 96 h of life to quantify total protein, DNA and RNA contents. In the jejunum and ileum, the highest levels of total protein and higher protein/RNA ratio were observed at 18 h (p < 0.05). There were no differences in DNA contents in any intestinal segment (p > 0.05). At 96 h, maximum levels of RNA were observed in the jejunum and ileum (p < 0.05) and higher RNA/DNA ratio in the three intestinal segments (p < 0.05), showing increased ability to synthesize intracellular RNA and proteins. The LBC group showed higher protein content and higher protein/DNA and protein/RNA ratios in the jejunum, a higher DNA content in the liver (p < 0.05) and a higher protein/RNA ratio in the muscle tissue (p < 0.05). In the muscle, higher protein and DNA levels were also found at 96 h (p < 0.05). Indicators of cellular activity suggest greater absorption of proteins from lyophilized bovine colostrum and increased cell maturity in the enteric and muscle tissues in the first hours of goat kids' life.

Cellular activity and development of enteric, hepatic and muscle tissues of juvenile pacuPiaractus mesopotamicus(Holmberg 1887) fed with lyophilized bovine colostrum

“…In pigs’ and goat kids suckled colostrum, Burrin, Shulman, Reeds, Davis and Gravitt () and Moretti, Nordi, Lima et al . () verified the increase of intracellular protein content influenced by absorption process of immunoglobulin besides translation activity. The process of protein synthesis in pacu′s intestine appears not be directly involved with fish growth, but with the absorptive processes of nutrients derived from food and metabolism.…”

“…In newborn goat kids fed with LBC, higher protein content and protein/DNA ratio were observed, indicating that the process of immunoglobulin internalization was intense in the small intestine (Moretti, Nordi, Lima et al . ). According with the results of the present study probably occurs stabilization through equivalence between proliferation and apoptosis rates in the enteric and hepatic tissues of fish, process observed too in ruminants by Bagaldo et al .…”

“…() and Moretti, Nordi, Lima et al . (). A possibility that can interfere in absorptive capacity by enteric cells in fish is the presence of enzymatic activity although that was not fully stimulated in juvenile pacu intestine (Moretti, Nordi, Cruz et al .…”

“…In the same way, higher value of protein/RNA ratio was observed in muscle of goat kids after the ingestion of bovine colostrum, positively related to tissue development (Moretti, Nordi, Lima et al . ).…”

“…Recently, a scientific topic of interest is the effects of bovine colostrum as a dietary supplement incorporate in fish diet. Colostrum is a rich source of protein like immunoglobulins, essential nutrients, hormones and biologically active molecules and is widely used in mammals as a source of nutrition and immune protection to newborn (Blum & Baumrucker ; Hurley & Theil ; Moretti, Nordi, Lima, Pauletti, Susin & Machado‐Neto ; Lima, Moretti, Nordi, Pauletti, Susin & Machado‐Neto ; Moretti, Nordi, Lima, Pauletti & Machado‐Neto ). The consumption of bovine colostrum by fish provided intestinal morphometrical alteration, as a consequence of increased activity of absorption processes (Rodrigues, Pauletti, Kindlein, Delgado, Cyrino & Machado‐Neto ), changes in pattern of goblet cells present in enteric mucosae (Cruz, Moretti, Nordi, Cyrino & Machado‐Neto ), the indicators of cellular activity in muscle, liver and intestine tissue (Pauletti, Kindlein, Bagaldo, Rodrigues, Delgado, Cyrino & Machado‐Neto ,b) and was found too absence of implications in intestinal enzymatic activity (Moretti, Nordi, Cruz, Cyrino & Machado‐Neto ).…”