Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

Havenith, Carin E.G.; Haarst, J. M. W. Van; Breedijk, Annette; Betjes, Michiel G. H.; Hoogsteden, H. C.; Beelen, Robert H.J.; Hoefsmit, E. C. M.

doi:10.1111/j.1365-2249.1994.tb06564.x

Cited by 14 publications

(5 citation statements)

References 36 publications

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“…DCs purified from normal BAL induce 5 to 10 times more T cell proliferation than alveolar macrophages. This result has been replicated using multiple DC purification techniques including ''high-tech'' bead selection and cell sorting (63), and ''low-tech'' density gradient fractionation (43,64,65). While DCs have not yet been isolated from sarcoidosis BAL fluid for functional studies, it is likely that they are more immunostimulatory than sarcoidosis alveolar macrophages.…”

Section: Dcs Are More Immunostimulatory Than Macrophagesmentioning

confidence: 87%

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Dendritic Cells in the Pathogenesis of Sarcoidosis

Zaba¹,

Smith²,

Sanchez³

et al. 2010

Am J Respir Cell Mol Biol

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Section: Dcs Are More Immunostimulatory Than Macrophagesmentioning

confidence: 87%

“…DCs only constitute approximately 1% of BAL fluid, as defined by lower FSC and SSC, and high expression of both MHC class II receptor HLA-DR and myeloid lineage integrin CD11c (34,43). This relative paucity of DCs in the BAL does not, however, reflect a lack of DCs in the airway.…”

Section: Myeloid Dendritic Cells In Sarcoidosis Dcs In Bal Fluid Are mentioning

confidence: 98%

Dendritic Cells in the Pathogenesis of Sarcoidosis

Zaba¹,

Smith²,

Sanchez³

et al. 2010

Am J Respir Cell Mol Biol

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“…In an early report involving anergic sarcoidosis patients, reduced numbers of epidermal Langerhans cells were found in both lesional and non-involved skin, with the most marked reductions seen in patients with multi-systemic disease (28). Later investigators have detected DCs in bronchoalveolar washings from sarcoidosis patients (29). More recently, it was shown that airway mDCs from sarcoidosis patients had increased expression of CD80 and decreased expression of CD86 compared to normal healthy volunteers, although overall DC numbers were comparable between the two groups (30).…”

Section: Discussionmentioning

confidence: 99%

The Anergic State in Sarcoidosis Is Associated with Diminished Dendritic Cell Function

Mathew

Bauer

Fischoeder

et al. 2008

The Journal of Immunology

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Sarcoidosis is a chronic inflammatory disease of unknown cause, characterized by granuloma formation similar to tuberculosis, but without clear evidence of a microbial infection. Because sarcoidosis is linked with clinical anergy and other evidence of diminished cellular immunity, we hypothesized that decreased skin delayed-type hypersensitivity (DTH) responses to recall Ags in affected individuals would be associated with decreased function of their blood dendritic cells (DCs). Our study involved ex vivo isolation, phenotyping, and functional testing of myeloid DCs (mDCs), plasmacytoid DCs, and T lymphocytes from blood of normal healthy volunteers and sarcoidosis subjects with active, untreated pulmonary disease. We found mDC function in the allogeneic MLR directly corresponded to the magnitude of skin DTH reactions to recall Ags in both sarcoidosis subjects and normal volunteers. However, both of these outcomes were significantly decreased in the sarcoidosis group. Diminished mDC function occurred despite up-regulated costimulatory and maturation markers. Clinical relevance is suggested by the inverse relationship between both mDC allogeneic responses and skin DTH responses with clinical disease severity as measured by chest radiograms. Because granulomas form when cellular immunity fails to clear antigenic stimuli, attenuated mDC function in sarcoidosis may contribute to susceptibility and persistence of the chronic inflammation characteristic of this disease.

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“…Pulmonary DCs (18), which modulate the asthmatic inflammation (17), were recently characterized in the human lung (18)(19)(20)(21)(22). The functional subset of myeloid DCs (mDCs) plays a key role in inducing and maintaining an adaptive immune response to allergens in the lung (23).…”

Section: What This Study Adds To the Fieldmentioning

confidence: 99%

Endotoxin Augments Myeloid Dendritic Cell Influx into the Airways in Patients with Allergic Asthma

Schaumann¹,

Müller²,

Braun³

et al. 2008

Am J Respir Crit Care Med

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Rationale: Epidemiologic studies have shown that exacerbation of asthma is modulated by environmental endotoxin. High levels of endotoxin are associated with asthma symptoms and the current use of asthma medication. However, the underlying mechanisms by which endotoxin modulates asthma are not completely understood. Objectives: The aim of the study was to test whether endotoxin enhances the response of individuals with allergic asthma to allergen, and to determine if this interaction is associated with increased numbers of antigen-presenting cells in the airways. Methods: Seventeen subjects with mild allergic asthma underwent segmental challenge with allergen, endotoxin, and the combination of both in three different lung segments via bronchoscopy. The cellular influx including monocytes, myeloid dendritic cells (mDCs), and plasmacytoid dendritic cells (pDCs), as well as the level of cytokines, were assessed in bronchoalveolar lavage fluid obtained 24 hours after segmental challenge. Monocytes, mDCs, and pDCs were isolated and their capacity to induce T cell proliferation was determined. Measurements and Main Results: Endotoxin enhanced the cellular response to allergen. The combination of allergen and endotoxin resulted in increased numbers of total cells, lymphocytes, neutrophils, eosinophils, monocytes, and mDCs, as well as increased levels of lipopolysaccharide-binding protein, IL-1a, IL-6, and tumor necrosis factor-a in the bronchoalveolar lavage fluid compared with allergen alone. Isolated mDCs but not pDCs induced a strong T cell proliferation in vitro. Conclusions: Endotoxin augments the allergic inflammation in the lungs of individuals with asthma, and induces an enhanced influx of monocytes and functionally active antigen-presenting mDCs into the respiratory tract.

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Enrichment and characterization of dendritic cells from human bronchoalveolar lavages

Cited by 14 publications

References 36 publications

Dendritic Cells in the Pathogenesis of Sarcoidosis

Dendritic Cells in the Pathogenesis of Sarcoidosis

The Anergic State in Sarcoidosis Is Associated with Diminished Dendritic Cell Function

Endotoxin Augments Myeloid Dendritic Cell Influx into the Airways in Patients with Allergic Asthma

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